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https://doi.org/10.21256/zhaw-1462| Publication type: | Article in scientific journal |
| Type of review: | Peer review (publication) |
| Title: | Fire blight disease reactome : RNA-seq transcriptional profile of apple host plant defence responses to Erwinia amylovora pathogen infection |
| Authors: | Kamber, Tim Buchmann, Jan P. Pothier, Joël Smits, Theo Wicker, Thomas Duffy, Brion |
| DOI: | 10.21256/zhaw-1462 10.1038/srep21600 |
| Published in: | Scientific Reports |
| Volume(Issue): | 6 |
| Issue: | 21600 |
| Issue Date: | 17-Feb-2016 |
| Publisher / Ed. Institution: | Nature |
| Publisher / Ed. Institution: | London |
| ISSN: | 2045-2322 |
| Language: | English |
| Subjects: | Transcriptomics; Feuerbrand; Erwinia |
| Subject (DDC): | 570: Biology |
| Abstract: | The molecular basis of resistance and susceptibility of host plants to fire blight, a major disease threat to pome fruit production globally, is largely unknown. RNA-sequencing data from challenged and mock-inoculated flowers were analyzed to assess the susceptible response of apple to the fire blight pathogen Erwinia amylovora. In presence of the pathogen 1,080 transcripts were differentially expressed at 48 h post inoculation. These included putative disease resistance, stress, pathogen related, general metabolic, and phytohormone related genes. Reads, mapped to regions on the apple genome where no genes were assigned, were used to identify potential novel genes and open reading frames. To identify transcripts specifically expressed in response to E. amylovora, RT-PCRs were conducted and compared to the expression patterns of the fire blight biocontrol agent Pantoea vagans strain C9-1, another apple pathogen Pseudomonas syringae pv. papulans, and mock inoculated apple flowers. This led to the identification of a peroxidase superfamily gene that was lower expressed in response to E. amylovora suggesting a potential role in the susceptibility response. Overall, this study provides the first transcriptional profile by RNA-seq of the host plant during fire blight disease and insights into the response of susceptible apple plants to E. amylovora. |
| URI: | https://digitalcollection.zhaw.ch/handle/11475/1561 |
| Fulltext version: | Published version |
| License (according to publishing contract): | Licence according to publishing contract |
| Departement: | Life Sciences and Facility Management |
| Organisational Unit: | Institute of Natural Resource Sciences (IUNR) |
| Published as part of the ZHAW project: | Genomik und Transkriptomik von Erwinia und Pantoea |
| Appears in collections: | Publikationen Life Sciences und Facility Management |
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| File | Description | Size | Format | |
|---|---|---|---|---|
| 2016_Kamber_Fire blight disease reactome_Scientific Reports.pdf | 690.96 kB | Adobe PDF |  View/Open |
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Kamber, T., Buchmann, J. P., Pothier, J., Smits, T., Wicker, T., & Duffy, B. (2016). Fire blight disease reactome : RNA-seq transcriptional profile of apple host plant defence responses to Erwinia amylovora pathogen infection. Scientific Reports, 6(21600). https://doi.org/10.21256/zhaw-1462
Kamber, T. et al. (2016) ‘Fire blight disease reactome : RNA-seq transcriptional profile of apple host plant defence responses to Erwinia amylovora pathogen infection’, Scientific Reports, 6(21600). Available at: https://doi.org/10.21256/zhaw-1462.
T. Kamber, J. P. Buchmann, J. Pothier, T. Smits, T. Wicker, and B. Duffy, “Fire blight disease reactome : RNA-seq transcriptional profile of apple host plant defence responses to Erwinia amylovora pathogen infection,” Scientific Reports, vol. 6, no. 21600, Feb. 2016, doi: 10.21256/zhaw-1462.
KAMBER, Tim, Jan P. BUCHMANN, Joël POTHIER, Theo SMITS, Thomas WICKER und Brion DUFFY, 2016. Fire blight disease reactome : RNA-seq transcriptional profile of apple host plant defence responses to Erwinia amylovora pathogen infection. Scientific Reports. 17 Februar 2016. Bd. 6, Nr. 21600. DOI 10.21256/zhaw-1462
Kamber, Tim, Jan P. Buchmann, Joël Pothier, Theo Smits, Thomas Wicker, and Brion Duffy. 2016. “Fire Blight Disease Reactome : RNA-Seq Transcriptional Profile of Apple Host Plant Defence Responses to Erwinia Amylovora Pathogen Infection.” Scientific Reports 6 (21600). https://doi.org/10.21256/zhaw-1462.
Kamber, Tim, et al. “Fire Blight Disease Reactome : RNA-Seq Transcriptional Profile of Apple Host Plant Defence Responses to Erwinia Amylovora Pathogen Infection.” Scientific Reports, vol. 6, no. 21600, Feb. 2016, https://doi.org/10.21256/zhaw-1462.
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