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Title: Fire blight disease reactome : RNA-seq transcriptional profile of apple host plant defence responses to Erwinia amylovora pathogen infection
Authors : Kamber, Tim
Buchmann, Jan P.
Pothier, Joël
Smits, Theo
Wicker, Thomas
Duffy, Brion
Published in : Scientific Reports
Volume(Issue) : 6
Issue : 21600
Publisher / Ed. Institution : Nature Publishing Group
Publisher / Ed. Institution: London
Issue Date: 17-Feb-2016
License (according to publishing contract) : Licence according to publishing contract
Type of review: Not specified
Language : English
Subjects : Transcriptomics; Feuerbrand; Erwinia
Subject (DDC) : 570: Biology
Abstract: The molecular basis of resistance and susceptibility of host plants to fire blight, a major disease threat to pome fruit production globally, is largely unknown. RNA-sequencing data from challenged and mock-inoculated flowers were analyzed to assess the susceptible response of apple to the fire blight pathogen Erwinia amylovora. In presence of the pathogen 1,080 transcripts were differentially expressed at 48 h post inoculation. These included putative disease resistance, stress, pathogen related, general metabolic, and phytohormone related genes. Reads, mapped to regions on the apple genome where no genes were assigned, were used to identify potential novel genes and open reading frames. To identify transcripts specifically expressed in response to E. amylovora, RT-PCRs were conducted and compared to the expression patterns of the fire blight biocontrol agent Pantoea vagans strain C9-1, another apple pathogen Pseudomonas syringae pv. papulans, and mock inoculated apple flowers. This led to the identification of a peroxidase superfamily gene that was lower expressed in response to E. amylovora suggesting a potential role in the susceptibility response. Overall, this study provides the first transcriptional profile by RNA-seq of the host plant during fire blight disease and insights into the response of susceptible apple plants to E. amylovora.
Departement: Life Sciences and Facility Management
Organisational Unit: Institute of Natural Resource Sciences (IUNR)
Publication type: Article in scientific Journal
DOI : 10.1038/srep21600
ISSN: 2045-2322
Published as part of the ZHAW project : Genomik und Transkriptomik von Erwinia und Pantoea
Appears in Collections:Publikationen Life Sciences und Facility Management

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