| Publikationstyp: | Beitrag in wissenschaftlicher Zeitschrift |
| Art der Begutachtung: | Peer review (Publikation) |
| Titel: | Respiratory syncytial virus subunit vaccine based on a recombinant fusion protein expressed transiently in mammalian cells |
| Autor/-in: | Nallet, Sophie Amacker, Mario Westerfeld, Nicole Baldi, Lucia König, Iwo Hacker, David L. Zaborosch, Christiane Zurbriggen, Rinaldo Wurm, Florian M. |
| DOI: | 10.1016/j.vaccine.2009.06.019 |
| Erschienen in: | Vaccine |
| Band(Heft): | 27 |
| Heft: | 46 |
| Seite(n): | 6415 |
| Seiten bis: | 6419 |
| Erscheinungsdatum: | 2009 |
| Verlag / Hrsg. Institution: | Elsevier |
| ISSN: | 0264-410X 1873-2518 |
| Sprache: | Englisch |
| Schlagwörter: | Viral antibodies; Cell line; Inbred BALB C mice; Neutralization tests; Recombinant fusion proteins; Respiratory syncytial virus infections; Respiratory syncytial virus vaccines; Subunit vaccines; Viral fusion proteins; Virosomes; Transfection |
| Fachgebiet (DDC): | 615: Pharmakologie und Therapeutik |
| Zusammenfassung: | Although respiratory syncytial virus (RSV) causes severe lower respiratory tract infection in infants and adults at risk, no RSV vaccine is currently available. In this report, efforts toward the generation of an RSV subunit vaccine using recombinant RSV fusion protein (rRSV-F) are described. The recombinant protein was produced by transient gene expression (TGE) in suspension-adapted human embryonic kidney cells (HEK-293E) in 4 L orbitally shaken bioreactors. It was then purified and formulated in immunostimulating reconstituted influenza virosomes (IRIVs). The candidate vaccine induced anti-RSV-F neutralizing antibodies in mice, and challenge studies in cotton rats are ongoing. If successful in preclinical and clinical trials, this will be the first recombinant subunit vaccine produced by large-scale TGE in mammalian cells. |
| URI: | https://digitalcollection.zhaw.ch/handle/11475/15015 |
| Volltext Version: | Publizierte Version |
| Lizenz (gemäss Verlagsvertrag): | Lizenz gemäss Verlagsvertrag |
| Departement: | Life Sciences und Facility Management |
| Organisationseinheit: | Institut für Chemie und Biotechnologie (ICBT) |
| Enthalten in den Sammlungen: | Publikationen Life Sciences und Facility Management |
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Nallet, S., Amacker, M., Westerfeld, N., Baldi, L., König, I., Hacker, D. L., Zaborosch, C., Zurbriggen, R., & Wurm, F. M. (2009). Respiratory syncytial virus subunit vaccine based on a recombinant fusion protein expressed transiently in mammalian cells. Vaccine, 27(46), 6415–6419. https://doi.org/10.1016/j.vaccine.2009.06.019
Nallet, S. et al. (2009) ‘Respiratory syncytial virus subunit vaccine based on a recombinant fusion protein expressed transiently in mammalian cells’, Vaccine, 27(46), pp. 6415–6419. Available at: https://doi.org/10.1016/j.vaccine.2009.06.019.
S. Nallet et al., “Respiratory syncytial virus subunit vaccine based on a recombinant fusion protein expressed transiently in mammalian cells,” Vaccine, vol. 27, no. 46, pp. 6415–6419, 2009, doi: 10.1016/j.vaccine.2009.06.019.
NALLET, Sophie, Mario AMACKER, Nicole WESTERFELD, Lucia BALDI, Iwo KÖNIG, David L. HACKER, Christiane ZABOROSCH, Rinaldo ZURBRIGGEN und Florian M. WURM, 2009. Respiratory syncytial virus subunit vaccine based on a recombinant fusion protein expressed transiently in mammalian cells. Vaccine. 2009. Bd. 27, Nr. 46, S. 6415–6419. DOI 10.1016/j.vaccine.2009.06.019
Nallet, Sophie, Mario Amacker, Nicole Westerfeld, Lucia Baldi, Iwo König, David L. Hacker, Christiane Zaborosch, Rinaldo Zurbriggen, and Florian M. Wurm. 2009. “Respiratory Syncytial Virus Subunit Vaccine Based on a Recombinant Fusion Protein Expressed Transiently in Mammalian Cells.” Vaccine 27 (46): 6415–19. https://doi.org/10.1016/j.vaccine.2009.06.019.
Nallet, Sophie, et al. “Respiratory Syncytial Virus Subunit Vaccine Based on a Recombinant Fusion Protein Expressed Transiently in Mammalian Cells.” Vaccine, vol. 27, no. 46, 2009, pp. 6415–19, https://doi.org/10.1016/j.vaccine.2009.06.019.
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