Publication type: Article in scientific journal
Type of review: Peer review (publication)
Title: Unraveling the mechanisms involved in zearalenone-mediated toxicity in permanent fish cell cultures
Authors : Pietsch-Schmied, Constanze
Noser, Jürg
Wettstein, Felix E.
Burkhardt-Holm, Patricia
DOI : 10.1016/j.toxicon.2014.06.005
Published in : Toxicon
Volume(Issue) : 88
Pages : 44
Pages to: 61
Issue Date: 2014
Publisher / Ed. Institution : Elsevier
ISSN: 0041-0101
Language : English
Subjects : Cytotoxicity; Estrogenic action; Genotoxicity; Mycotoxin; Oxidative stress; Animal; Cell survival; Cultured cells; Drug dose-response relationship; Estrogen receptor alpha; Estrogen receptor beta; Glutathione; Oncorhynchus mykiss; Salmon; Zearalenone
Subject (DDC) : 571: Physiology and related subjects
Abstract: The world-wide occurrence of zearalenone (ZEN) as a contaminant in feed for farm animals and fish requires the evaluation of toxicity mechanisms of action of ZEN. The present study investigates possible metabolization of ZEN in fish cell lines suggesting that mainly glucuronidation takes place. It demonstrates that concentrations up to 20,000 ng ml(-1) ZEN are capable of influencing cell viability in permanent fish cell cultures in a dose-response manner with different response patterns between the five tested cell lines, whereby lysosomes appeared to be the main target of ZEN. ZEN toxicity is often discussed in the context of oxidative stress. Our study shows a biphasic response of the cell lines when reactive oxygen species (ROS) production is monitored. Damage in cells was observed by measuring lipid peroxidation, DNA strand breaks, and alterations of intracellular glutathione levels. Metabolization of ZEN, especially at concentrations above 7500 ng ml(-1) ZEN, does not prevent cytotoxicity. ZEN as an estrogenic compound may involve processes mediated by binding to estrogen receptors (ER). Since one cell line showed no detectable expression of ER, an ER-mediated pathway seems to be unlikely in these cells. This confirms a lysosomal pathway as a main target of ZEN in fish cells.
Fulltext version : Published version
License (according to publishing contract) : Licence according to publishing contract
Departement: Life Sciences and Facility Management
Appears in Collections:Publikationen Life Sciences und Facility Management

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