| Publikationstyp: | Beitrag in wissenschaftlicher Zeitschrift |
| Art der Begutachtung: | Peer review (Publikation) |
| Titel: | Multiparameter microscopy and spectroscopy for single-molecule analytics |
| Autor/-in: | Prummer, Michael Sick, Beate Renn, Alois Wild, Urs P. |
| DOI: | 10.1021/ac034976g |
| Erschienen in: | Analytical Chemistry |
| Band(Heft): | 76 |
| Heft: | 6 |
| Seite(n): | 1633 |
| Seiten bis: | 1640 |
| Erscheinungsdatum: | 2004 |
| Verlag / Hrsg. Institution: | American Chemical Society |
| ISSN: | 0003-2700 1520-6882 |
| Sprache: | Englisch |
| Schlagwörter: | Optics; Bayes statistics; Single molecule; Multi parameter |
| Fachgebiet (DDC): | 540: Chemie |
| Zusammenfassung: | The ability to monitor several parameters simultaneously from distinct individual fluorescent reporter molecules facilitates the disentanglement of complex and interacting systems and opens new perspectives in areas from basic science to biopharmaceutical technology. By combining annular illumination microscopy, time-correlated single photon counting, and multichannel detection, we were able to determine 14 independent parameters from one individual fluorophore. The whole set of parameters was deduced from the few properties of the fluorescence photons, i.e., arrival time, wavelength, and polarization. With this approach, the intensity, the polarization, and the spectral dynamics can be analyzed on a nanosecond time scale and the mean values can be monitored with submillisecond time resolution. Nanosecond spectral dynamics of single molecules has been observed, to the best of our knowledge, for the first time. From our experience, we can determine all parameters for more than 30% of the illuminated fluorophores in biological samples and for more than 80% in doped polymeric films. |
| URI: | https://digitalcollection.zhaw.ch/handle/11475/14032 |
| Volltext Version: | Publizierte Version |
| Lizenz (gemäss Verlagsvertrag): | Lizenz gemäss Verlagsvertrag |
| Departement: | School of Engineering |
| Organisationseinheit: | Institut für Datenanalyse und Prozessdesign (IDP) |
| Enthalten in den Sammlungen: | Publikationen School of Engineering |
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Prummer, M., Sick, B., Renn, A., & Wild, U. P. (2004). Multiparameter microscopy and spectroscopy for single-molecule analytics. Analytical Chemistry, 76(6), 1633–1640. https://doi.org/10.1021/ac034976g
Prummer, M. et al. (2004) ‘Multiparameter microscopy and spectroscopy for single-molecule analytics’, Analytical Chemistry, 76(6), pp. 1633–1640. Available at: https://doi.org/10.1021/ac034976g.
M. Prummer, B. Sick, A. Renn, and U. P. Wild, “Multiparameter microscopy and spectroscopy for single-molecule analytics,” Analytical Chemistry, vol. 76, no. 6, pp. 1633–1640, 2004, doi: 10.1021/ac034976g.
PRUMMER, Michael, Beate SICK, Alois RENN und Urs P. WILD, 2004. Multiparameter microscopy and spectroscopy for single-molecule analytics. Analytical Chemistry. 2004. Bd. 76, Nr. 6, S. 1633–1640. DOI 10.1021/ac034976g
Prummer, Michael, Beate Sick, Alois Renn, and Urs P. Wild. 2004. “Multiparameter Microscopy and Spectroscopy for Single-Molecule Analytics.” Analytical Chemistry 76 (6): 1633–40. https://doi.org/10.1021/ac034976g.
Prummer, Michael, et al. “Multiparameter Microscopy and Spectroscopy for Single-Molecule Analytics.” Analytical Chemistry, vol. 76, no. 6, 2004, pp. 1633–40, https://doi.org/10.1021/ac034976g.
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