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dc.contributor.authorPrummer, Michael-
dc.contributor.authorSick, Beate-
dc.contributor.authorRenn, Alois-
dc.contributor.authorWild, Urs P.-
dc.date.accessioned2018-12-20T09:04:44Z-
dc.date.available2018-12-20T09:04:44Z-
dc.date.issued2004-
dc.identifier.issn0003-2700de_CH
dc.identifier.issn1520-6882de_CH
dc.identifier.urihttps://digitalcollection.zhaw.ch/handle/11475/14032-
dc.description.abstractThe ability to monitor several parameters simultaneously from distinct individual fluorescent reporter molecules facilitates the disentanglement of complex and interacting systems and opens new perspectives in areas from basic science to biopharmaceutical technology. By combining annular illumination microscopy, time-correlated single photon counting, and multichannel detection, we were able to determine 14 independent parameters from one individual fluorophore. The whole set of parameters was deduced from the few properties of the fluorescence photons, i.e., arrival time, wavelength, and polarization. With this approach, the intensity, the polarization, and the spectral dynamics can be analyzed on a nanosecond time scale and the mean values can be monitored with submillisecond time resolution. Nanosecond spectral dynamics of single molecules has been observed, to the best of our knowledge, for the first time. From our experience, we can determine all parameters for more than 30% of the illuminated fluorophores in biological samples and for more than 80% in doped polymeric films.de_CH
dc.language.isoende_CH
dc.publisherAmerican Chemical Societyde_CH
dc.relation.ispartofAnalytical Chemistryde_CH
dc.rightsLicence according to publishing contractde_CH
dc.subjectOpticsde_CH
dc.subjectBayes statisticsde_CH
dc.subjectSingle moleculede_CH
dc.subjectMulti parameterde_CH
dc.subject.ddc540: Chemiede_CH
dc.titleMultiparameter microscopy and spectroscopy for single-molecule analyticsde_CH
dc.typeBeitrag in wissenschaftlicher Zeitschriftde_CH
dcterms.typeTextde_CH
zhaw.departementSchool of Engineeringde_CH
zhaw.organisationalunitInstitut für Datenanalyse und Prozessdesign (IDP)de_CH
dc.identifier.doi10.1021/ac034976gde_CH
zhaw.funding.euNode_CH
zhaw.issue6de_CH
zhaw.originated.zhawNode_CH
zhaw.pages.end1640de_CH
zhaw.pages.start1633de_CH
zhaw.publication.statuspublishedVersionde_CH
zhaw.volume76de_CH
zhaw.publication.reviewPeer review (Publikation)de_CH
Appears in collections:Publikationen School of Engineering

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Prummer, M., Sick, B., Renn, A., & Wild, U. P. (2004). Multiparameter microscopy and spectroscopy for single-molecule analytics. Analytical Chemistry, 76(6), 1633–1640. https://doi.org/10.1021/ac034976g
Prummer, M. et al. (2004) ‘Multiparameter microscopy and spectroscopy for single-molecule analytics’, Analytical Chemistry, 76(6), pp. 1633–1640. Available at: https://doi.org/10.1021/ac034976g.
M. Prummer, B. Sick, A. Renn, and U. P. Wild, “Multiparameter microscopy and spectroscopy for single-molecule analytics,” Analytical Chemistry, vol. 76, no. 6, pp. 1633–1640, 2004, doi: 10.1021/ac034976g.
PRUMMER, Michael, Beate SICK, Alois RENN und Urs P. WILD, 2004. Multiparameter microscopy and spectroscopy for single-molecule analytics. Analytical Chemistry. 2004. Bd. 76, Nr. 6, S. 1633–1640. DOI 10.1021/ac034976g
Prummer, Michael, Beate Sick, Alois Renn, and Urs P. Wild. 2004. “Multiparameter Microscopy and Spectroscopy for Single-Molecule Analytics.” Analytical Chemistry 76 (6): 1633–40. https://doi.org/10.1021/ac034976g.
Prummer, Michael, et al. “Multiparameter Microscopy and Spectroscopy for Single-Molecule Analytics.” Analytical Chemistry, vol. 76, no. 6, 2004, pp. 1633–40, https://doi.org/10.1021/ac034976g.


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