Title: Probing confined fields with single molecules and vice versa
Authors : Sick, Beate
Hecht, B.
Wild, U.P.
Novotny, L.
Published in : Journal of Microscopy
Volume(Issue) : 202
Issue : 2
Pages : 365
Pages to: 373
Publisher / Ed. Institution : Wiley
Issue Date: 2001
License (according to publishing contract) : Licence according to publishing contract
Type of review: Peer review (publication)
Language : English
Subject (DDC) : 570: Biology
Abstract: Single dye molecules are used as local probes to map the spatial distribution of the squared electric field components in the focus of a high numerical aperture lens. Simulated field distributions are quantitatively verified by experimentally obtained fluorescence excitation maps. We show that annular illumination can be used to engineer the field distribution in the focus at a dielectric/air interface such that electric field components in all directions acquire comparable magnitudes. The 3D orientation of molecular absorption dipoles can be determined by comparing measured to simulated image patterns. The presence of longitudinal electric field components in a focus is of particular interest in tip-enhanced scanning near-field optical microscopy.
Departement: School of Engineering
Organisational Unit: Institute of Data Analysis and Process Design (IDP)
Publication type: Article in scientific journal
DOI : 10.1046/j.1365-2818.2001.00795.x
ISSN: 0022-2720
1365-2818
URI: https://digitalcollection.zhaw.ch/handle/11475/14003
Appears in Collections:Publikationen School of Engineering

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