Please use this identifier to cite or link to this item: https://doi.org/10.21256/zhaw-4761
Publication type: Article in scientific journal
Type of review: Peer review (publication)
Title: Rhamnose-inducible gene expression in Listeria monocytogenes
Authors: Fieseler, Lars
Schmitter, Sibylle
Teiserskas, Justinas
Loessner, Martin J.
DOI: 10.21256/zhaw-4761
10.1371/journal.pone.0043444
Published in: PLOS ONE
Volume(Issue): 7
Issue: 8
Pages: e43444
Issue Date: 2012
Publisher / Ed. Institution: Public Library of Science
ISSN: 1932-6203
Language: English
Subjects: Cell line; Bacterial gene expression regulation; Bacterial genes; Reporter genes; Genetic vectors; Intracellular space; Listeria monocytogenes; Macrophages; Genetic promoter regions; Rhamnose; Genetic transcription
Subject (DDC): 570: Biology
Abstract: Acid production from rhamnose is a characteristic phenotype of Listeria monocytogenes. We report the identification of the rhamnose transport and utilization operon located at lmo2846 to lmo2851, including the rhamnose-dependent promoter P(rha). Expression of reporter genes under control of P(rha) on a single copy integration vector demonstrated its suitability for inducible gene expression in L. monocytogenes. Transcription initiation from P(rha) is dose dependent, and a concentration as low as 100 µM rhamnose was found sufficient for induction. Moreover, P(rha) is subject to glucose catabolite repression, which provides additional options for strict control of expression. Infection of human THP1 macrophages revealed that P(rha) is repressed in intracellular L. monocytogenes, which is explained by the absence of rhamnose in the cytosol and possible interference by catabolite repression. The P(rha) promoter provides a novel and useful tool for triggering gene expression in extracellular L. monocytogenes, whereas intracellular conditions prevent transcription from this promoter.
URI: https://digitalcollection.zhaw.ch/handle/11475/12269
Fulltext version: Published version
License (according to publishing contract): CC BY 4.0: Attribution 4.0 International
Departement: Life Sciences and Facility Management
Organisational Unit: Institute of Food and Beverage Innovation (ILGI)
Appears in collections:Publikationen Life Sciences und Facility Management

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