Please use this identifier to cite or link to this item:
https://doi.org/10.21256/zhaw-4761
Publication type: | Article in scientific journal |
Type of review: | Peer review (publication) |
Title: | Rhamnose-inducible gene expression in Listeria monocytogenes |
Authors: | Fieseler, Lars Schmitter, Sibylle Teiserskas, Justinas Loessner, Martin J. |
DOI: | 10.21256/zhaw-4761 10.1371/journal.pone.0043444 |
Published in: | PLOS ONE |
Volume(Issue): | 7 |
Issue: | 8 |
Page(s): | e43444 |
Issue Date: | 2012 |
Publisher / Ed. Institution: | Public Library of Science |
ISSN: | 1932-6203 |
Language: | English |
Subjects: | Cell line; Bacterial gene expression regulation; Bacterial genes; Reporter genes; Genetic vectors; Intracellular space; Listeria monocytogenes; Macrophages; Genetic promoter regions; Rhamnose; Genetic transcription |
Subject (DDC): | 570: Biology |
Abstract: | Acid production from rhamnose is a characteristic phenotype of Listeria monocytogenes. We report the identification of the rhamnose transport and utilization operon located at lmo2846 to lmo2851, including the rhamnose-dependent promoter P(rha). Expression of reporter genes under control of P(rha) on a single copy integration vector demonstrated its suitability for inducible gene expression in L. monocytogenes. Transcription initiation from P(rha) is dose dependent, and a concentration as low as 100 µM rhamnose was found sufficient for induction. Moreover, P(rha) is subject to glucose catabolite repression, which provides additional options for strict control of expression. Infection of human THP1 macrophages revealed that P(rha) is repressed in intracellular L. monocytogenes, which is explained by the absence of rhamnose in the cytosol and possible interference by catabolite repression. The P(rha) promoter provides a novel and useful tool for triggering gene expression in extracellular L. monocytogenes, whereas intracellular conditions prevent transcription from this promoter. |
URI: | https://digitalcollection.zhaw.ch/handle/11475/12269 |
Fulltext version: | Published version |
License (according to publishing contract): | CC BY 4.0: Attribution 4.0 International |
Departement: | Life Sciences and Facility Management |
Organisational Unit: | Institute of Food and Beverage Innovation (ILGI) |
Appears in collections: | Publikationen Life Sciences und Facility Management |
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Fieseler, L., Schmitter, S., Teiserskas, J., & Loessner, M. J. (2012). Rhamnose-inducible gene expression in Listeria monocytogenes. Plos One, 7(8), e43444. https://doi.org/10.21256/zhaw-4761
Fieseler, L. et al. (2012) ‘Rhamnose-inducible gene expression in Listeria monocytogenes’, PLOS ONE, 7(8), p. e43444. Available at: https://doi.org/10.21256/zhaw-4761.
L. Fieseler, S. Schmitter, J. Teiserskas, and M. J. Loessner, “Rhamnose-inducible gene expression in Listeria monocytogenes,” PLOS ONE, vol. 7, no. 8, p. e43444, 2012, doi: 10.21256/zhaw-4761.
FIESELER, Lars, Sibylle SCHMITTER, Justinas TEISERSKAS und Martin J. LOESSNER, 2012. Rhamnose-inducible gene expression in Listeria monocytogenes. PLOS ONE. 2012. Bd. 7, Nr. 8, S. e43444. DOI 10.21256/zhaw-4761
Fieseler, Lars, Sibylle Schmitter, Justinas Teiserskas, and Martin J. Loessner. 2012. “Rhamnose-Inducible Gene Expression in Listeria Monocytogenes.” Plos One 7 (8): e43444. https://doi.org/10.21256/zhaw-4761.
Fieseler, Lars, et al. “Rhamnose-Inducible Gene Expression in Listeria Monocytogenes.” Plos One, vol. 7, no. 8, 2012, p. e43444, https://doi.org/10.21256/zhaw-4761.
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