Publication type: Article in scientific journal
Type of review: Peer review (publication)
Title: Synergistic rate boosting of collagen fibrillogenesis in heterogeneous mixtures of crowding agents
Authors: Dewavrin, Jean-Yves
Abdurrahiem, Muhammed
Blocki, Anna
Musib, Mrinal
Piazza, Francesco
Raghunath, Michael
DOI: 10.1021/jp5077559
Published in: Journal of Physical Chemistry B
Volume(Issue): 119
Issue: 12
Pages: 4350
Pages to: 4358
Issue Date: 2015
Publisher / Ed. Institution: American Chemical Society
ISSN: 1520-6106
Language: English
Subject (DDC): 571: Physiology and related subjects
572: Biochemistry
Abstract: The competition for access to space that arises between macromolecules is the basis of the macromolecular crowding phenomenon, known to modulate biochemical reactions in subtle ways. Crowding is a highly conserved physiological condition in and around cells in metazoans, and originates from a mixture of heterogeneous biomolecules. Here, using collagen fibrillogenesis as an experimental test platform and ideas from the theory of nonideal solutions, we show that an entropy-based synergy is created by a mixture of two different populations of artificial crowders, providing small crowders with extra volume occupancy when in the vicinity of bigger crowders. We present the physiological mechanism by which synergistic effects maximize volume exclusion with the minimum amount of heterogeneous crowders, demonstrating how the evolutionarily optimized crowded conditions found in vivo can be reproduced effectively in vitro.
Fulltext version: Published version
License (according to publishing contract): Licence according to publishing contract
Departement: Life Sciences and Facility Management
Organisational Unit: Institute of Chemistry and Biotechnology (ICBT)
Appears in collections:Publikationen Life Sciences und Facility Management

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