ECM microenvironment unlocks brown adipogenic potential of adult human bone marrow-derived MSCs

H. Lee, Michelle; Goralczyk, Anna G.; Kriszt, Rókus; Ang, Xiu Min; Badowski, Cedric; Li, Ying; Summers, Scott A.; Toh, Sue-Anne; Yassin, M. Shabeer; Shabbir, Asim; Sheppard, Allan; Raghunath, Michael

doi:10.21256/zhaw-4752

Please use this identifier to cite or link to this item: https://doi.org/10.21256/zhaw-4752

Publication type:	Article in scientific journal
Type of review:	Peer review (publication)
Title:	ECM microenvironment unlocks brown adipogenic potential of adult human bone marrow-derived MSCs
Authors:	H. Lee, Michelle Goralczyk, Anna G. Kriszt, Rókus Ang, Xiu Min Badowski, Cedric Li, Ying Summers, Scott A. Toh, Sue-Anne Yassin, M. Shabeer Shabbir, Asim Sheppard, Allan Raghunath, Michael
DOI:	10.21256/zhaw-4752 10.1038/srep21173
Published in:	Scientific Reports
Volume(Issue):	6
Issue:	21173
Issue Date:	2016
Publisher / Ed. Institution:	Nature Publishing Group
ISSN:	2045-2322
Language:	English
Subject (DDC):	571: Physiology and related subjects 610: Medicine and health
Abstract:	Key to realizing the diagnostic and therapeutic potential of human brown/brite adipocytes is the identification of a renewable, easily accessible and safe tissue source of progenitor cells, and an efficacious in vitro differentiation protocol. We show that macromolecular crowding (MMC) facilitates brown adipocyte differentiation in adult human bone marrow mesenchymal stem cells (bmMSCs), as evidenced by substantially upregulating uncoupling protein 1 (UCP1) and uncoupled respiration. Moreover, MMC also induced ‘browning’ in bmMSC-derived white adipocytes. Mechanistically, MMC creates a 3D extracellular matrix architecture enshrouding maturing adipocytes in a collagen IV cocoon that is engaged by paxillin-positive focal adhesions also at the apical side of cells, without contact to the stiff support structure. This leads to an enhanced matrix-cell signaling, reflected by increased phosphorylation of ATF2, a key transcription factor in UCP1 regulation. Thus, tuning the dimensionality of the microenvironment in vitro can unlock a strong brown potential dormant in bone marrow.
URI:	https://digitalcollection.zhaw.ch/handle/11475/12195
Fulltext version:	Published version
License (according to publishing contract):	CC BY 4.0: Attribution 4.0 International
Departement:	Life Sciences and Facility Management
Organisational Unit:	Institute of Chemistry and Biotechnology (ICBT)
Appears in collections:	Publikationen Life Sciences und Facility Management

Files in This Item:

File	Description	Size	Format
2016_Lee_et_al_ECM_microenvironment_unlocks_brown_adipogenic_potential.pdf		3.71 MB	Adobe PDF	View/Open

Show full item record

H. Lee, M., Goralczyk, A. G., Kriszt, R., Ang, X. M., Badowski, C., Li, Y., Summers, S. A., Toh, S.-A., Yassin, M. S., Shabbir, A., Sheppard, A., & Raghunath, M. (2016). ECM microenvironment unlocks brown adipogenic potential of adult human bone marrow-derived MSCs. Scientific Reports, 6(21173). https://doi.org/10.21256/zhaw-4752

H. Lee, M. et al. (2016) ‘ECM microenvironment unlocks brown adipogenic potential of adult human bone marrow-derived MSCs’, Scientific Reports, 6(21173). Available at: https://doi.org/10.21256/zhaw-4752.

M. H. Lee et al., “ECM microenvironment unlocks brown adipogenic potential of adult human bone marrow-derived MSCs,” Scientific Reports, vol. 6, no. 21173, 2016, doi: 10.21256/zhaw-4752.

H. LEE, Michelle, Anna G. GORALCZYK, Rókus KRISZT, Xiu Min ANG, Cedric BADOWSKI, Ying LI, Scott A. SUMMERS, Sue-Anne TOH, M. Shabeer YASSIN, Asim SHABBIR, Allan SHEPPARD und Michael RAGHUNATH, 2016. ECM microenvironment unlocks brown adipogenic potential of adult human bone marrow-derived MSCs. Scientific Reports. 2016. Bd. 6, Nr. 21173. DOI 10.21256/zhaw-4752

H. Lee, Michelle, Anna G. Goralczyk, Rókus Kriszt, Xiu Min Ang, Cedric Badowski, Ying Li, Scott A. Summers, et al. 2016. “ECM Microenvironment Unlocks Brown Adipogenic Potential of Adult Human Bone Marrow-Derived MSCs.” Scientific Reports 6 (21173). https://doi.org/10.21256/zhaw-4752.

H. Lee, Michelle, et al. “ECM Microenvironment Unlocks Brown Adipogenic Potential of Adult Human Bone Marrow-Derived MSCs.” Scientific Reports, vol. 6, no. 21173, 2016, https://doi.org/10.21256/zhaw-4752.