Please use this identifier to cite or link to this item: https://doi.org/10.21256/zhaw-4752
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dc.contributor.authorH. Lee, Michelle-
dc.contributor.authorGoralczyk, Anna G.-
dc.contributor.authorKriszt, Rókus-
dc.contributor.authorAng, Xiu Min-
dc.contributor.authorBadowski, Cedric-
dc.contributor.authorLi, Ying-
dc.contributor.authorSummers, Scott A.-
dc.contributor.authorToh, Sue-Anne-
dc.contributor.authorYassin, M. Shabeer-
dc.contributor.authorShabbir, Asim-
dc.contributor.authorSheppard, Allan-
dc.contributor.authorRaghunath, Michael-
dc.date.accessioned2018-10-26T14:11:32Z-
dc.date.available2018-10-26T14:11:32Z-
dc.date.issued2016-
dc.identifier.issn2045-2322de_CH
dc.identifier.urihttps://digitalcollection.zhaw.ch/handle/11475/12195-
dc.description.abstractKey to realizing the diagnostic and therapeutic potential of human brown/brite adipocytes is the identification of a renewable, easily accessible and safe tissue source of progenitor cells, and an efficacious in vitro differentiation protocol. We show that macromolecular crowding (MMC) facilitates brown adipocyte differentiation in adult human bone marrow mesenchymal stem cells (bmMSCs), as evidenced by substantially upregulating uncoupling protein 1 (UCP1) and uncoupled respiration. Moreover, MMC also induced ‘browning’ in bmMSC-derived white adipocytes. Mechanistically, MMC creates a 3D extracellular matrix architecture enshrouding maturing adipocytes in a collagen IV cocoon that is engaged by paxillin-positive focal adhesions also at the apical side of cells, without contact to the stiff support structure. This leads to an enhanced matrix-cell signaling, reflected by increased phosphorylation of ATF2, a key transcription factor in UCP1 regulation. Thus, tuning the dimensionality of the microenvironment in vitro can unlock a strong brown potential dormant in bone marrow.de_CH
dc.language.isoende_CH
dc.publisherNature Publishing Groupde_CH
dc.relation.ispartofScientific Reportsde_CH
dc.rightshttp://creativecommons.org/licenses/by/4.0/de_CH
dc.subject.ddc571: Physiologie und verwandte Themende_CH
dc.subject.ddc610: Medizin und Gesundheitde_CH
dc.titleECM microenvironment unlocks brown adipogenic potential of adult human bone marrow-derived MSCsde_CH
dc.typeBeitrag in wissenschaftlicher Zeitschriftde_CH
dcterms.typeTextde_CH
zhaw.departementLife Sciences und Facility Managementde_CH
zhaw.organisationalunitInstitut für Chemie und Biotechnologie (ICBT)de_CH
dc.identifier.doi10.21256/zhaw-4752-
dc.identifier.doi10.1038/srep21173de_CH
zhaw.funding.euNode_CH
zhaw.issue21173de_CH
zhaw.originated.zhawNode_CH
zhaw.publication.statuspublishedVersionde_CH
zhaw.volume6de_CH
zhaw.publication.reviewPeer review (Publikation)de_CH
zhaw.webfeedMetabolic Tissue Engineeringde_CH
Appears in collections:Publikationen Life Sciences und Facility Management

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