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https://doi.org/10.21256/zhaw-4752| Publikationstyp: | Beitrag in wissenschaftlicher Zeitschrift |
| Art der Begutachtung: | Peer review (Publikation) |
| Titel: | ECM microenvironment unlocks brown adipogenic potential of adult human bone marrow-derived MSCs |
| Autor/-in: | H. Lee, Michelle Goralczyk, Anna G. Kriszt, Rókus Ang, Xiu Min Badowski, Cedric Li, Ying Summers, Scott A. Toh, Sue-Anne Yassin, M. Shabeer Shabbir, Asim Sheppard, Allan Raghunath, Michael |
| DOI: | 10.21256/zhaw-4752 10.1038/srep21173 |
| Erschienen in: | Scientific Reports |
| Band(Heft): | 6 |
| Heft: | 21173 |
| Erscheinungsdatum: | 2016 |
| Verlag / Hrsg. Institution: | Nature Publishing Group |
| ISSN: | 2045-2322 |
| Sprache: | Englisch |
| Fachgebiet (DDC): | 571: Physiologie und verwandte Themen 610: Medizin und Gesundheit |
| Zusammenfassung: | Key to realizing the diagnostic and therapeutic potential of human brown/brite adipocytes is the identification of a renewable, easily accessible and safe tissue source of progenitor cells, and an efficacious in vitro differentiation protocol. We show that macromolecular crowding (MMC) facilitates brown adipocyte differentiation in adult human bone marrow mesenchymal stem cells (bmMSCs), as evidenced by substantially upregulating uncoupling protein 1 (UCP1) and uncoupled respiration. Moreover, MMC also induced ‘browning’ in bmMSC-derived white adipocytes. Mechanistically, MMC creates a 3D extracellular matrix architecture enshrouding maturing adipocytes in a collagen IV cocoon that is engaged by paxillin-positive focal adhesions also at the apical side of cells, without contact to the stiff support structure. This leads to an enhanced matrix-cell signaling, reflected by increased phosphorylation of ATF2, a key transcription factor in UCP1 regulation. Thus, tuning the dimensionality of the microenvironment in vitro can unlock a strong brown potential dormant in bone marrow. |
| URI: | https://digitalcollection.zhaw.ch/handle/11475/12195 |
| Volltext Version: | Publizierte Version |
| Lizenz (gemäss Verlagsvertrag): | CC BY 4.0: Namensnennung 4.0 International |
| Departement: | Life Sciences und Facility Management |
| Organisationseinheit: | Institut für Chemie und Biotechnologie (ICBT) |
| Enthalten in den Sammlungen: | Publikationen Life Sciences und Facility Management |
Dateien zu dieser Ressource:
| Datei | Beschreibung | Größe | Format | |
|---|---|---|---|---|
| 2016_Lee_et_al_ECM_microenvironment_unlocks_brown_adipogenic_potential.pdf | 3.71 MB | Adobe PDF |  Öffnen/Anzeigen |
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H. Lee, M., Goralczyk, A. G., Kriszt, R., Ang, X. M., Badowski, C., Li, Y., Summers, S. A., Toh, S.-A., Yassin, M. S., Shabbir, A., Sheppard, A., & Raghunath, M. (2016). ECM microenvironment unlocks brown adipogenic potential of adult human bone marrow-derived MSCs. Scientific Reports, 6(21173). https://doi.org/10.21256/zhaw-4752
H. Lee, M. et al. (2016) ‘ECM microenvironment unlocks brown adipogenic potential of adult human bone marrow-derived MSCs’, Scientific Reports, 6(21173). Available at: https://doi.org/10.21256/zhaw-4752.
M. H. Lee et al., “ECM microenvironment unlocks brown adipogenic potential of adult human bone marrow-derived MSCs,” Scientific Reports, vol. 6, no. 21173, 2016, doi: 10.21256/zhaw-4752.
H. LEE, Michelle, Anna G. GORALCZYK, Rókus KRISZT, Xiu Min ANG, Cedric BADOWSKI, Ying LI, Scott A. SUMMERS, Sue-Anne TOH, M. Shabeer YASSIN, Asim SHABBIR, Allan SHEPPARD und Michael RAGHUNATH, 2016. ECM microenvironment unlocks brown adipogenic potential of adult human bone marrow-derived MSCs. Scientific Reports. 2016. Bd. 6, Nr. 21173. DOI 10.21256/zhaw-4752
H. Lee, Michelle, Anna G. Goralczyk, Rókus Kriszt, Xiu Min Ang, Cedric Badowski, Ying Li, Scott A. Summers, et al. 2016. “ECM Microenvironment Unlocks Brown Adipogenic Potential of Adult Human Bone Marrow-Derived MSCs.” Scientific Reports 6 (21173). https://doi.org/10.21256/zhaw-4752.
H. Lee, Michelle, et al. “ECM Microenvironment Unlocks Brown Adipogenic Potential of Adult Human Bone Marrow-Derived MSCs.” Scientific Reports, vol. 6, no. 21173, 2016, https://doi.org/10.21256/zhaw-4752.
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