Title: Sourcing of an alternative pericyte-like cell type from peripheral blood generated with pulsed macromolecular crowding
Authors : Blocki, Anna
Wang, Yingting
Koch, Maria
Goralczyk, Anna
Beyer, Sebastian
Agarwal, Nikita
Lee, Michelle
Moonshi, Shehzahdi
Dewavrin, Jean-Yves
Peh, Priscilla
Schwarz, Herbert
Bhakoo, Kishore
Raghunath, Michael
Published in : Molecular therapy
Volume(Issue) : 23
Issue : 3
Pages : 510
Pages to: 522
Publisher / Ed. Institution : Elsevier
Issue Date: 2015
License (according to publishing contract) : Licence according to publishing contract
Type of review: Peer review (Publication)
Language : English
Subject (DDC) : 610: Medicine and health
615: Pharmacology and therapeutics
Abstract: Autologous cells hold great potential for personalized cell therapy, reducing immunological and risk of infections. However, low cell counts at harvest with subsequently long expansion times with associated cell function loss currently impede the advancement of autologous cell therapy approaches. Here, we aimed to source clinically relevant numbers of proangiogenic cells from an easy accessible cell source, namely peripheral blood. Using macromolecular crowding (MMC) as a biotechnological platform, we derived a novel cell type from peripheral blood that is generated within 5 days in large numbers (10–40 million cells per 100 ml of blood). This blood-derived angiogenic cell (BDAC) type is of monocytic origin, but exhibits pericyte markers PDGFR-β and NG2 and demonstrates strong angiogenic activity, hitherto ascribed only to MSC-like pericytes. Our findings suggest that BDACs represent an alternative pericyte-like cell population of hematopoietic origin that is involved in promoting early stages of microvasculature formation. As a proof of principle of BDAC efficacy in an ischemic disease model, BDAC injection rescued affected tissues in a murine hind limb ischemia model by accelerating and enhancing revascularization. Derived from a renewable tissue that is easy to collect, BDACs overcome current short-comings of autologous cell therapy, in particular for tissue repair strategies.
Departement: Life Sciences and Facility Management
Organisational Unit: Institute of Chemistry and Biotechnology (ICBT)
Publication type: Article in scientific Journal
DOI : 10.1038/mt.2014.232
ISSN: 1525-0016
1525-0024
URI: https://digitalcollection.zhaw.ch/handle/11475/12182
Appears in Collections:Publikationen Life Sciences und Facility Management

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