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https://doi.org/10.21256/zhaw-4838| Publikationstyp: | Beitrag in wissenschaftlicher Zeitschrift |
| Art der Begutachtung: | Peer review (Publikation) |
| Titel: | Optical visualisation of thermogenesis in stimulated singlecell brown adipocytes |
| Autor/-in: | Kriszt, Rókus Arai, Satoshi Itoh, Hideki Lee, Michelle H. Goralczyk, Anna G. Ang, Xiu Min Cypess, Aaron M. White, Andrew P. Shamsi, Farnaz Xue, Ruidan Lee, Jung Yeol Lee, Sung-Chan Hou, Yanyan Kitaguchi, Tetsuya Sudhaharan, Thankiah Ishiwata, Shin’ichi Lane, E. Birgitte Chang, Young-Tae Tseng, Yu-Hua Suzuki, Madoka Raghunath, Michael |
| DOI: | 10.21256/zhaw-4838 10.1038/s41598-017-00291-9 |
| Erschienen in: | Scientific Reports |
| Erscheinungsdatum: | 2017 |
| Verlag / Hrsg. Institution: | Nature Publishing Group |
| ISSN: | 2045-2322 |
| Sprache: | Englisch |
| Fachgebiet (DDC): | 610: Medizin und Gesundheit |
| Zusammenfassung: | The identification of brown adipose deposits in adults has led to significant interest in targeting this metabolically active tissue for treatment of obesity and diabetes. Improved methods for the direct measurement of heat production as the signature function of brown adipocytes (BAs), particularly at the single cell level, would be of substantial benefit to these ongoing efforts. Here, we report the first application of a small molecule-type thermosensitive fluorescent dye, ERthermAC, to monitor thermogenesis in BAs derived from murine brown fat precursors and in human brown fat cells differentiated from human neck brown preadipocytes. ERthermAC accumulated in the endoplasmic reticulum of BAs and displayed a marked change in fluorescence intensity in response to adrenergic stimulation of cells, which corresponded to temperature change. ERthermAC fluorescence intensity profiles were congruent with mitochondrial depolarisation events visualised by the JC-1 probe. Moreover, the averaged fluorescence intensity changes across a population of cells correlated well with dynamic changes such as thermal power, oxygen consumption, and extracellular acidification rates. These findings suggest ERthermAC as a promising new tool for studying thermogenic function in brown adipocytes of both murine and human origins. |
| URI: | https://digitalcollection.zhaw.ch/handle/11475/12094 |
| Volltext Version: | Publizierte Version |
| Lizenz (gemäss Verlagsvertrag): | CC BY 4.0: Namensnennung 4.0 International |
| Departement: | Life Sciences und Facility Management |
| Organisationseinheit: | Institut für Chemie und Biotechnologie (ICBT) |
| Enthalten in den Sammlungen: | Publikationen Life Sciences und Facility Management |
Dateien zu dieser Ressource:
| Datei | Beschreibung | Größe | Format | |
|---|---|---|---|---|
| 2017_Kriszt_Optical_visualisation_of_thermogenesis_in_stimulated.pdf | 3.16 MB | Adobe PDF |  Öffnen/Anzeigen |
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Kriszt, R., Arai, S., Itoh, H., Lee, M. H., Goralczyk, A. G., Ang, X. M., Cypess, A. M., White, A. P., Shamsi, F., Xue, R., Lee, J. Y., Lee, S.-C., Hou, Y., Kitaguchi, T., Sudhaharan, T., Ishiwata, S., Lane, E. B., Chang, Y.-T., Tseng, Y.-H., et al. (2017). Optical visualisation of thermogenesis in stimulated singlecell brown adipocytes. Scientific Reports. https://doi.org/10.21256/zhaw-4838
Kriszt, R. et al. (2017) ‘Optical visualisation of thermogenesis in stimulated singlecell brown adipocytes’, Scientific Reports [Preprint]. Available at: https://doi.org/10.21256/zhaw-4838.
R. Kriszt et al., “Optical visualisation of thermogenesis in stimulated singlecell brown adipocytes,” Scientific Reports, 2017, doi: 10.21256/zhaw-4838.
KRISZT, Rókus, Satoshi ARAI, Hideki ITOH, Michelle H. LEE, Anna G. GORALCZYK, Xiu Min ANG, Aaron M. CYPESS, Andrew P. WHITE, Farnaz SHAMSI, Ruidan XUE, Jung Yeol LEE, Sung-Chan LEE, Yanyan HOU, Tetsuya KITAGUCHI, Thankiah SUDHAHARAN, Shin’ichi ISHIWATA, E. Birgitte LANE, Young-Tae CHANG, Yu-Hua TSENG, Madoka SUZUKI und Michael RAGHUNATH, 2017. Optical visualisation of thermogenesis in stimulated singlecell brown adipocytes. Scientific Reports. 2017. DOI 10.21256/zhaw-4838
Kriszt, Rókus, Satoshi Arai, Hideki Itoh, Michelle H. Lee, Anna G. Goralczyk, Xiu Min Ang, Aaron M. Cypess, et al. 2017. “Optical Visualisation of Thermogenesis in Stimulated Singlecell Brown Adipocytes.” Scientific Reports. https://doi.org/10.21256/zhaw-4838.
Kriszt, Rókus, et al. “Optical Visualisation of Thermogenesis in Stimulated Singlecell Brown Adipocytes.” Scientific Reports, 2017, https://doi.org/10.21256/zhaw-4838.
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