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Publication type: Article in scientific journal
Type of review: Peer review (publication)
Title: Optical visualisation of thermogenesis in stimulated singlecell brown adipocytes
Authors: Kriszt, Rókus
Arai, Satoshi
Itoh, Hideki
Lee, Michelle H.
Goralczyk, Anna G.
Ang, Xiu Min
Cypess, Aaron M.
White, Andrew P.
Shamsi, Farnaz
Xue, Ruidan
Lee, Jung Yeol
Lee, Sung-Chan
Hou, Yanyan
Kitaguchi, Tetsuya
Sudhaharan, Thankiah
Ishiwata, Shin’ichi
Lane, E. Birgitte
Chang, Young-Tae
Tseng, Yu-Hua
Suzuki, Madoka
Raghunath, Michael
DOI: 10.21256/zhaw-4838
Published in: Scientific Reports
Issue Date: 2017
Publisher / Ed. Institution: Nature Publishing Group
ISSN: 2045-2322
Language: English
Subject (DDC): 610: Medicine and health
Abstract: The identification of brown adipose deposits in adults has led to significant interest in targeting this metabolically active tissue for treatment of obesity and diabetes. Improved methods for the direct measurement of heat production as the signature function of brown adipocytes (BAs), particularly at the single cell level, would be of substantial benefit to these ongoing efforts. Here, we report the first application of a small molecule-type thermosensitive fluorescent dye, ERthermAC, to monitor thermogenesis in BAs derived from murine brown fat precursors and in human brown fat cells differentiated from human neck brown preadipocytes. ERthermAC accumulated in the endoplasmic reticulum of BAs and displayed a marked change in fluorescence intensity in response to adrenergic stimulation of cells, which corresponded to temperature change. ERthermAC fluorescence intensity profiles were congruent with mitochondrial depolarisation events visualised by the JC-1 probe. Moreover, the averaged fluorescence intensity changes across a population of cells correlated well with dynamic changes such as thermal power, oxygen consumption, and extracellular acidification rates. These findings suggest ERthermAC as a promising new tool for studying thermogenic function in brown adipocytes of both murine and human origins.
Fulltext version: Published version
License (according to publishing contract): CC BY 4.0: Attribution 4.0 International
Departement: Life Sciences and Facility Management
Organisational Unit: Institute of Chemistry and Biotechnology (ICBT)
Appears in Collections:Publikationen Life Sciences und Facility Management

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