Publikationstyp: Konferenz: Poster
Art der Begutachtung: Keine Angabe
Titel: Biofilms in metalworking fluids
Autor/-in: Berner, Florian
Inauen, Silvio
Rüfenacht, Andrea
Krebs, Walter
Angaben zur Konferenz: Biofilms IV - International Conference: «Communities Bridging Disciplines», Winchester, United Kingdom, 2010
Erscheinungsdatum: 2010
Sprache: Englisch
Schlagwörter: Kühlschmierstoffe; Biofilme; Mikrobiologie
Fachgebiet (DDC): 570: Biologie
Zusammenfassung: Metalworking fluids play a critical role whenever metal is shaped in modern tool machines. The cooling and lubrication provided by these oil-water emulsions are important factors in improving the quality of the product and the lifetime of the tools. As these machines are open systems, the metalworking fluids are colonized and eventually degraded by microorganisms. Consequently industrial research has a considerable interest in the microbiology of these systems. However, most previous studies have focused on the planctonic organisms of the liquid phase, with little work done on biofilms. In this CTI-funded project an existing industrial test reactor for metalworking fluids (designed by Blaser Swisslube AG) was adapted for biofilm cultivation. Biofilms were cultivated in different Blasocut®Bioconcept formulations, a metalworking fluid designed to maintain stability by allowing a dominant population of P. pseudoalcaligenes that prevents growth of other species. The reactors were monitored for up to eight weeks for planctonic and biofilm growth, which was quantified using classical cultivation methods and total mass. Growth was reproducible independent of the test coupons material (stainless steel or aluminium). Different biofilm quantities were shown for different Blasocut®Bioconcept formulations, strongly corresponding with planctonic bacterial densities. In order to study the microbial diversity of the biofilm and determine how it differed from the planctonic phase, samples were also analysed by mass spectroscopy (MALDI-TOF), quantitative PCR and denaturing gradient gel electrophoresis.
Volltext Version: Publizierte Version
Lizenz (gemäss Verlagsvertrag): Lizenz gemäss Verlagsvertrag
Departement: Life Sciences und Facility Management
Organisationseinheit: Institut für Chemie und Biotechnologie (ICBT)
Publiziert im Rahmen des ZHAW-Projekts: Analyse und Kontrolle von Biofilmen in Kühlschmierstoffsystemen
Enthalten in den Sammlungen:Publikationen Life Sciences und Facility Management

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