|Title:||Biofilms in metalworking fluids|
|Authors :||Berner, Florian|
|Conference details:||Biofilms IV - Communities Bridging Disciplines - 4th International Conference, Winchester, 2010|
|License (according to publishing contract) :||Licence according to publishing contract|
|Type of review:||Not specified|
|Subjects :||Kühlschmierstoffe; Biofilme; Mikrobiologie|
|Subject (DDC) :||570: Biology|
|Abstract:||Metalworking fluids play a critical role whenever metal is shaped in modern tool machines. The cooling and lubrication provided by these oil-water emulsions are important factors in improving the quality of the product and the lifetime of the tools. As these machines are open systems, the metalworking fluids are colonized and eventually degraded by microorganisms. Consequently industrial research has a considerable interest in the microbiology of these systems. However, most previous studies have focused on the planctonic organisms of the liquid phase, with little work done on biofilms. In this CTI-funded project an existing industrial test reactor for metalworking fluids (designed by Blaser Swisslube AG) was adapted for biofilm cultivation. Biofilms were cultivated in different Blasocut®Bioconcept formulations, a metalworking fluid designed to maintain stability by allowing a dominant population of P. pseudoalcaligenes that prevents growth of other species. The reactors were monitored for up to eight weeks for planctonic and biofilm growth, which was quantified using classical cultivation methods and total mass. Growth was reproducible independent of the test coupons material (stainless steel or aluminium). Different biofilm quantities were shown for different Blasocut®Bioconcept formulations, strongly corresponding with planctonic bacterial densities. In order to study the microbial diversity of the biofilm and determine how it differed from the planctonic phase, samples were also analysed by mass spectroscopy (MALDI-TOF), quantitative PCR and denaturing gradient gel electrophoresis.|
|Departement:||Life Sciences and Facility Management|
|Organisational Unit:||Institute of Chemistry and Biotechnology (ICBT)|
|Publication type:||Conference poster|
|Published as part of the ZHAW project :||Analyse und Kontrolle von Biofilmen in Kühlschmierstoffsystemen|
|Appears in Collections:||Publikationen Life Sciences und Facility Management|
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