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dc.contributor.authorGubesch, Michaela-
dc.contributor.authorTheler, Barbara-
dc.contributor.authorMathis, Alex-
dc.contributor.authorDutta, Manjula-
dc.contributor.authorBaumer, Beatrice-
dc.contributor.authorHolzhauser, Thomas-
dc.contributor.authorVieths, Stefan-
dc.contributor.authorBallmer-Weber, Barbara K.-
dc.date.accessioned2018-08-23T06:11:57Z-
dc.date.available2018-08-23T06:11:57Z-
dc.date.issued2007-
dc.identifier.issn0105-4538de_CH
dc.identifier.issn1398-9995de_CH
dc.identifier.urihttps://digitalcollection.zhaw.ch/handle/11475/9436-
dc.description.abstractBackground: Foods not commonly consumed in the European Union (EU) must be proven save before brought to market, including an assessment of allergenicity. We present a three-stepwise strategy for allergenicity assessment of natural novel foods using three novel vegetables as example (water spinach, hyacinth bean, Ethiopian eggplant). Methods: First, vegetable extracts were analysed for the presence of pan-allergens [Bet v 1 homologous proteins, profilins, non specific lipid transfer proteins (LTP)] by immunoblot analysis with specific animal antibodies. Second, the IgE-binding of the food extracts was investigated by EAST (Enzyme-allergosorbent test) and immunoblot analysis using sera with IgE-reactivity to known pan-allergens or to phylogenetically related foods from subjects 1) allergic to birch, grass and mugwort pollen, 2) with food allergy to soy, peanut, tomato, multiple pollen-related foods and 3) sensitised to LTP. Third, the clinical relevance of IgE-binding was assessed in vivo by skin prick testing (SPT) and open oral food challenges (OFC). Results: Profilin and LTP were detected by animal antibodies in all vegetables, a Bet v 1 homologue selectively in hyacinth bean. IgE-binding to LTP, profilin and a Bet v 1 homologue was proven by immunoblot analysis and EAST. Positive SPT and OFC results were observed for all vegetables in pollen-allergic patients. Conclusion: Our stepwise procedure confirmed the presence and IgE-binding capacity of novel vegetable proteins homologous to known allergens in endemic vegetable foods. In vivo testing proved the potential of the novel vegetables to elicit clinical allergy. Hence, our described algorithm seems to be applicable for allergenicity testing of natural novel foods.de_CH
dc.language.isoende_CH
dc.publisherWileyde_CH
dc.relation.ispartofAllergyde_CH
dc.rightsLicence according to publishing contractde_CH
dc.subjectFoodde_CH
dc.subjectPotentialde_CH
dc.subjectAllergenicde_CH
dc.subjectAllergyde_CH
dc.subject.ddc610: Medizin und Gesundheitde_CH
dc.titleStrategy for allergenicity assessment of "natural novel foods" : clinical and molecular investigation of exotic vegetables (water spinach, hyacinth bean and Ethiopian eggplant)de_CH
dc.typeBeitrag in wissenschaftlicher Zeitschriftde_CH
dcterms.typeTextde_CH
zhaw.departementLife Sciences und Facility Managementde_CH
zhaw.organisationalunitInstitut für Lebensmittel- und Getränkeinnovation (ILGI)de_CH
dc.identifier.doi10.1111/j.1398-9995.2007.01474.xde_CH
zhaw.funding.euNode_CH
zhaw.issue11de_CH
zhaw.originated.zhawYesde_CH
zhaw.pages.end1250de_CH
zhaw.pages.start1243de_CH
zhaw.publication.statuspublishedVersionde_CH
zhaw.volume62de_CH
zhaw.publication.reviewPeer review (Publikation)de_CH
zhaw.webfeedLM-Technologiede_CH
zhaw.webfeedHortikulturde_CH
Appears in collections:Publikationen Life Sciences und Facility Management

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Gubesch, M., Theler, B., Mathis, A., Dutta, M., Baumer, B., Holzhauser, T., Vieths, S., & Ballmer-Weber, B. K. (2007). Strategy for allergenicity assessment of “natural novel foods” : clinical and molecular investigation of exotic vegetables (water spinach, hyacinth bean and Ethiopian eggplant). Allergy, 62(11), 1243–1250. https://doi.org/10.1111/j.1398-9995.2007.01474.x
Gubesch, M. et al. (2007) ‘Strategy for allergenicity assessment of ‘natural novel foods’ : clinical and molecular investigation of exotic vegetables (water spinach, hyacinth bean and Ethiopian eggplant)’, Allergy, 62(11), pp. 1243–1250. Available at: https://doi.org/10.1111/j.1398-9995.2007.01474.x.
M. Gubesch et al., “Strategy for allergenicity assessment of “natural novel foods” : clinical and molecular investigation of exotic vegetables (water spinach, hyacinth bean and Ethiopian eggplant),” Allergy, vol. 62, no. 11, pp. 1243–1250, 2007, doi: 10.1111/j.1398-9995.2007.01474.x.
GUBESCH, Michaela, Barbara THELER, Alex MATHIS, Manjula DUTTA, Beatrice BAUMER, Thomas HOLZHAUSER, Stefan VIETHS und Barbara K. BALLMER-WEBER, 2007. Strategy for allergenicity assessment of „natural novel foods“ : clinical and molecular investigation of exotic vegetables (water spinach, hyacinth bean and Ethiopian eggplant). Allergy. 2007. Bd. 62, Nr. 11, S. 1243–1250. DOI 10.1111/j.1398-9995.2007.01474.x
Gubesch, Michaela, Barbara Theler, Alex Mathis, Manjula Dutta, Beatrice Baumer, Thomas Holzhauser, Stefan Vieths, and Barbara K. Ballmer-Weber. 2007. “Strategy for Allergenicity Assessment of “Natural Novel Foods” : Clinical and Molecular Investigation of Exotic Vegetables (Water Spinach, Hyacinth Bean and Ethiopian Eggplant).” Allergy 62 (11): 1243–50. https://doi.org/10.1111/j.1398-9995.2007.01474.x.
Gubesch, Michaela, et al. “Strategy for Allergenicity Assessment of “Natural Novel Foods” : Clinical and Molecular Investigation of Exotic Vegetables (Water Spinach, Hyacinth Bean and Ethiopian Eggplant).” Allergy, vol. 62, no. 11, 2007, pp. 1243–50, https://doi.org/10.1111/j.1398-9995.2007.01474.x.


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