Molecular analysis of a novel gene cluster encoding an insect toxin in plant-associated strains of Pseudomonas fluorescens

Péchy-Tarr, M.; Bruck, D. J.; Maurhofer, M.; Fischer, Esther; Vogne, C.; Henkels, M. D.; Donahue, K. M.; Grunder, Jürg; Loper, J. E.; Keel, C.

doi:10.1111/j.1462-2920.2008.01662.x

Full metadata record

DC Field	Value	Language
dc.contributor.author	Péchy-Tarr, M.	-
dc.contributor.author	Bruck, D. J.	-
dc.contributor.author	Maurhofer, M.	-
dc.contributor.author	Fischer, Esther	-
dc.contributor.author	Vogne, C.	-
dc.contributor.author	Henkels, M. D.	-
dc.contributor.author	Donahue, K. M.	-
dc.contributor.author	Grunder, Jürg	-
dc.contributor.author	Loper, J. E.	-
dc.contributor.author	Keel, C.	-
dc.date.accessioned	2018-07-04T14:08:47Z	-
dc.date.available	2018-07-04T14:08:47Z	-
dc.date.issued	2008	-
dc.identifier.issn	1462-2920	de_CH
dc.identifier.issn	1462-2912	de_CH
dc.identifier.uri	https://digitalcollection.zhaw.ch/handle/11475/7568	-
dc.description.abstract	Pseudomonas fluorescens CHA0 and the related strain Pf-5 are well-characterized representatives of rhizosphere bacteria that have the capacity to protect crop plants from fungal root diseases, mainly by releasing a variety of exoproducts that are toxic to plant pathogenic fungi. Here, we report that the two plant-beneficial pseudomonads also exhibit potent insecticidal activity. Anti-insect activity is linked to a novel genomic locus encoding a large protein toxin termed Fit (for P. fluorescensinsecticidal toxin) that is related to the insect toxin Mcf (Makes caterpillars floppy) of the entomopathogen Photorhabdus luminescens, a mutualist of insect-invading nematodes. When injected into the haemocoel, even low doses of P. fluorescens CHA0 or Pf-5 killed larvae of the tobacco hornworm Manduca sexta and the greater wax moth Galleria mellonella. In contrast, mutants of CHA0 or Pf-5 with deletions in the Fit toxin gene were significantly less virulent to the larvae. When expressed from an inducible promoter in a non-toxic Escherichia coli host, the Fit toxin gene was sufficient to render the bacterium toxic to both insect hosts. Our findings establish the Fit gene products of P. fluorescens CHA0 and Pf-5 as potent insect toxins that define previously unappreciated anti-insect properties of these plant-colonizing bacteria.	de_CH
dc.language.iso	en	de_CH
dc.publisher	Wiley	de_CH
dc.relation.ispartof	Environmental Microbiology	de_CH
dc.rights	Licence according to publishing contract	de_CH
dc.subject	Phytopathology	de_CH
dc.subject	Bacterial toxin	de_CH
dc.subject.ddc	630: Landwirtschaft	de_CH
dc.title	Molecular analysis of a novel gene cluster encoding an insect toxin in plant-associated strains of Pseudomonas fluorescens	de_CH
dc.type	Beitrag in wissenschaftlicher Zeitschrift	de_CH
dcterms.type	Text	de_CH
zhaw.departement	Life Sciences und Facility Management	de_CH
zhaw.organisationalunit	Institut für Umwelt und Natürliche Ressourcen (IUNR)	de_CH
dc.identifier.doi	10.1111/j.1462-2920.2008.01662.x	de_CH
zhaw.funding.eu	No	de_CH
zhaw.issue	9	de_CH
zhaw.originated.zhaw	Yes	de_CH
zhaw.pages.end	2386	de_CH
zhaw.pages.start	2368	de_CH
zhaw.publication.status	publishedVersion	de_CH
zhaw.volume	10	de_CH
zhaw.publication.review	Peer review (Publikation)	de_CH
zhaw.webfeed	Phytomedizin	de_CH
Appears in collections:	Publikationen Life Sciences und Facility Management

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Péchy-Tarr, M., Bruck, D. J., Maurhofer, M., Fischer, E., Vogne, C., Henkels, M. D., Donahue, K. M., Grunder, J., Loper, J. E., & Keel, C. (2008). Molecular analysis of a novel gene cluster encoding an insect toxin in plant-associated strains of Pseudomonas fluorescens. Environmental Microbiology, 10(9), 2368–2386. https://doi.org/10.1111/j.1462-2920.2008.01662.x

Péchy-Tarr, M. et al. (2008) ‘Molecular analysis of a novel gene cluster encoding an insect toxin in plant-associated strains of Pseudomonas fluorescens’, Environmental Microbiology, 10(9), pp. 2368–2386. Available at: https://doi.org/10.1111/j.1462-2920.2008.01662.x.

M. Péchy-Tarr et al., “Molecular analysis of a novel gene cluster encoding an insect toxin in plant-associated strains of Pseudomonas fluorescens,” Environmental Microbiology, vol. 10, no. 9, pp. 2368–2386, 2008, doi: 10.1111/j.1462-2920.2008.01662.x.

PÉCHY-TARR, M., D. J. BRUCK, M. MAURHOFER, Esther FISCHER, C. VOGNE, M. D. HENKELS, K. M. DONAHUE, Jürg GRUNDER, J. E. LOPER und C. KEEL, 2008. Molecular analysis of a novel gene cluster encoding an insect toxin in plant-associated strains of Pseudomonas fluorescens. Environmental Microbiology. 2008. Bd. 10, Nr. 9, S. 2368–2386. DOI 10.1111/j.1462-2920.2008.01662.x

Péchy-Tarr, M., D. J. Bruck, M. Maurhofer, Esther Fischer, C. Vogne, M. D. Henkels, K. M. Donahue, Jürg Grunder, J. E. Loper, and C. Keel. 2008. “Molecular Analysis of a Novel Gene Cluster Encoding an Insect Toxin in Plant-Associated Strains of Pseudomonas Fluorescens.” Environmental Microbiology 10 (9): 2368–86. https://doi.org/10.1111/j.1462-2920.2008.01662.x.

Péchy-Tarr, M., et al. “Molecular Analysis of a Novel Gene Cluster Encoding an Insect Toxin in Plant-Associated Strains of Pseudomonas Fluorescens.” Environmental Microbiology, vol. 10, no. 9, 2008, pp. 2368–86, https://doi.org/10.1111/j.1462-2920.2008.01662.x.