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dc.contributor.authorYildirim, Selçuk-
dc.contributor.authorRuinatscha, Reto-
dc.contributor.authorGross, Rainer-
dc.contributor.authorWohlgemuth, Roland-
dc.contributor.authorKohler, Hans-Peter E.-
dc.contributor.authorWitholt, Bernard-
dc.contributor.authorSchmid, Andreas-
dc.date.accessioned2018-06-18T13:52:43Z-
dc.date.available2018-06-18T13:52:43Z-
dc.date.issued2006-02-
dc.identifier.issn1381-1177de_CH
dc.identifier.urihttps://digitalcollection.zhaw.ch/handle/11475/7074-
dc.description.abstractSeveral nitrilases were screened for the hydrolysis of the nitrile group of cis-1,2-dihydroxy-3-cyanocyclohexa-3,5-diene and trans-3-[(5S,6R)-5,6-dihydroxycyclohexa-1,3-dienyl]-acrylonitrile to the corresponding acids. Nitrilase from Rhodococcus sp. was able to convert both compounds with the activity of 0.3 mU/mg protein and 0.05 mU/mg protein, respectively. Nitrilase AtNIT1 from Arabidopsis thaliana converted only the latter but with a higher initial specific activity of 1.7 mU/mg protein. Biotransformation of trans-3-[(5S,6R)-5,6-dihydroxycyclohexa-1,3-dienyl]-acrylonitrile was performed with AtNIT1 in the form of isolated enzyme and immobilized enzyme, and with recombinant cells containing AtNIT1. Biotransformations with isolated AtNIT1 resulted in 116 mg of product in 10.6 h with a yield of 77%. Forty-three percent of the enzymatic activity could be recovered after the biotransformation. Immobilization of AtNIT1 saturated with 3-phenylpropionitrile resulted in 3.5% of the free enzyme activity. Biotransformations with Escherischia coli JM101 (pQE10-AtNIT1) in shake-flasks produced 243 mg of product in 23 h with a yield of 48%. Maximum and average specific activities of 0.5 U/g cell dry weight and 0.17 U/g cell dry weight were achieved, respectively.de_CH
dc.language.isoende_CH
dc.publisherElsevierde_CH
dc.relation.ispartofJournal of Molecular Catalysis B: Enzymaticde_CH
dc.rightsLicence according to publishing contractde_CH
dc.subjectDihydrodiolde_CH
dc.subjectNitrilede_CH
dc.subjectEnzymede_CH
dc.subjectBiocatalysisde_CH
dc.subject.ddc572: Biochemiede_CH
dc.subject.ddc660.6: Biotechnologiede_CH
dc.titleSelective hydrolysis of the nitrile group of cis-dihydrodiols from aromatic nitrilesde_CH
dc.typeBeitrag in wissenschaftlicher Zeitschriftde_CH
dcterms.typeTextde_CH
zhaw.departementLife Sciences und Facility Managementde_CH
zhaw.organisationalunitInstitut für Lebensmittel- und Getränkeinnovation (ILGI)de_CH
dc.identifier.doi10.1016/j.molcatb.2005.11.006de_CH
zhaw.funding.euNode_CH
zhaw.issue2de_CH
zhaw.originated.zhawNode_CH
zhaw.pages.end83de_CH
zhaw.pages.start76de_CH
zhaw.publication.statuspublishedVersionde_CH
zhaw.volume38de_CH
zhaw.publication.reviewPeer review (Publikation)de_CH
zhaw.webfeedLM-Verpackungde_CH
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Yildirim, S., Ruinatscha, R., Gross, R., Wohlgemuth, R., Kohler, H.-P. E., Witholt, B., & Schmid, A. (2006). Selective hydrolysis of the nitrile group of cis-dihydrodiols from aromatic nitriles. Journal of Molecular Catalysis B: Enzymatic, 38(2), 76–83. https://doi.org/10.1016/j.molcatb.2005.11.006
Yildirim, S. et al. (2006) ‘Selective hydrolysis of the nitrile group of cis-dihydrodiols from aromatic nitriles’, Journal of Molecular Catalysis B: Enzymatic, 38(2), pp. 76–83. Available at: https://doi.org/10.1016/j.molcatb.2005.11.006.
S. Yildirim et al., “Selective hydrolysis of the nitrile group of cis-dihydrodiols from aromatic nitriles,” Journal of Molecular Catalysis B: Enzymatic, vol. 38, no. 2, pp. 76–83, Feb. 2006, doi: 10.1016/j.molcatb.2005.11.006.
YILDIRIM, Selçuk, Reto RUINATSCHA, Rainer GROSS, Roland WOHLGEMUTH, Hans-Peter E. KOHLER, Bernard WITHOLT und Andreas SCHMID, 2006. Selective hydrolysis of the nitrile group of cis-dihydrodiols from aromatic nitriles. Journal of Molecular Catalysis B: Enzymatic. Februar 2006. Bd. 38, Nr. 2, S. 76–83. DOI 10.1016/j.molcatb.2005.11.006
Yildirim, Selçuk, Reto Ruinatscha, Rainer Gross, Roland Wohlgemuth, Hans-Peter E. Kohler, Bernard Witholt, and Andreas Schmid. 2006. “Selective Hydrolysis of the Nitrile Group of Cis-Dihydrodiols from Aromatic Nitriles.” Journal of Molecular Catalysis B: Enzymatic 38 (2): 76–83. https://doi.org/10.1016/j.molcatb.2005.11.006.
Yildirim, Selçuk, et al. “Selective Hydrolysis of the Nitrile Group of Cis-Dihydrodiols from Aromatic Nitriles.” Journal of Molecular Catalysis B: Enzymatic, vol. 38, no. 2, Feb. 2006, pp. 76–83, https://doi.org/10.1016/j.molcatb.2005.11.006.


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