Dissection of a novel molecular determinant mediating Golgi to trans-Golgi network transition

Schaub, Beat E.; Berger, Eric G.; Rohrer, Jack

doi:10.1007/s00018-008-8446-y

Please use this identifier to cite or link to this item: https://doi.org/10.21256/zhaw-6803

Publication type:	Article in scientific journal
Type of review:	Peer review (publication)
Title:	Dissection of a novel molecular determinant mediating Golgi to trans-Golgi network transition
Authors:	Schaub, Beat E. Berger, Eric G. Rohrer, Jack
DOI:	10.1007/s00018-008-8446-y 10.21256/zhaw-6803
Published in:	Cellular and Molecular Life Sciences
Volume(Issue):	65
Issue:	22
Page(s):	3677
Pages to:	3687
Issue Date:	Nov-2008
Publisher / Ed. Institution:	Springer
ISSN:	1420-9071 1420-682X
Language:	English
Subjects:	Golgi Apparatus; Sorting signal; Trafficking; Glycosyltransferases; Monensin
Subject (DDC):	571: Physiology and related subjects
Abstract:	Two major functions of the Golgi apparatus (GA) are formation of complex glycans and sorting of proteins destined for various subcellular compartments or secretion. To fulfill these tasks proper localization of the accessory proteins within the different sub-compartments of the GA is crucial. Here we investigate structural determinants mediating transition of the two glycosyltransferases beta-1,4- galactosyltransferase 1 (gal-T1) and the alpha-1,3-fucosyltransferase 6 (fuc-T6) from the trans-Golgi cisterna to the trans-Golgi network (TGN). Upon treatment with the ionophore monensin both glycosyltransferases are found in TGN-derived swollen vesicles, as determined by confocal fluorescence microscopy and density gradient fractionation. Both enzymes carry a signal consisting of the amino acids E(5)P(6) in gal-T1 and D(2)P(3) in fuc-T6 necessary for the transition of these glycosyltransferases from the trans-Golgi cisterna to the TGN, but not for their steady state localization in the trans-Golgi cisterna.
URI:	https://digitalcollection.zhaw.ch/handle/11475/6803
Fulltext version:	Published version
License (according to publishing contract):	Licence according to publishing contract
Departement:	Life Sciences and Facility Management
Organisational Unit:	Institute of Chemistry and Biotechnology (ICBT)
Appears in collections:	Publikationen Life Sciences und Facility Management

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Schaub, B. E., Berger, E. G., & Rohrer, J. (2008). Dissection of a novel molecular determinant mediating Golgi to trans-Golgi network transition. Cellular and Molecular Life Sciences, 65(22), 3677–3687. https://doi.org/10.1007/s00018-008-8446-y

Schaub, B.E., Berger, E.G. and Rohrer, J. (2008) ‘Dissection of a novel molecular determinant mediating Golgi to trans-Golgi network transition’, Cellular and Molecular Life Sciences, 65(22), pp. 3677–3687. Available at: https://doi.org/10.1007/s00018-008-8446-y.

B. E. Schaub, E. G. Berger, and J. Rohrer, “Dissection of a novel molecular determinant mediating Golgi to trans-Golgi network transition,” Cellular and Molecular Life Sciences, vol. 65, no. 22, pp. 3677–3687, Nov. 2008, doi: 10.1007/s00018-008-8446-y.

SCHAUB, Beat E., Eric G. BERGER und Jack ROHRER, 2008. Dissection of a novel molecular determinant mediating Golgi to trans-Golgi network transition. Cellular and Molecular Life Sciences. November 2008. Bd. 65, Nr. 22, S. 3677–3687. DOI 10.1007/s00018-008-8446-y

Schaub, Beat E., Eric G. Berger, and Jack Rohrer. 2008. “Dissection of a Novel Molecular Determinant Mediating Golgi to Trans-Golgi Network Transition.” Cellular and Molecular Life Sciences 65 (22): 3677–87. https://doi.org/10.1007/s00018-008-8446-y.

Schaub, Beat E., et al. “Dissection of a Novel Molecular Determinant Mediating Golgi to Trans-Golgi Network Transition.” Cellular and Molecular Life Sciences, vol. 65, no. 22, Nov. 2008, pp. 3677–87, https://doi.org/10.1007/s00018-008-8446-y.