| Publikationstyp: | Beitrag in wissenschaftlicher Zeitschrift |
| Art der Begutachtung: | Peer review (Publikation) |
| Titel: | Determination of the structural requirements for palmitoylation of p63 |
| Autor/-in: | Schweizer, Anja Rohrer, Jack Kornfeld, Stuart |
| DOI: | 10.1074/jbc.270.16.9638 |
| Erschienen in: | Journal of Biological Chemistry |
| Band(Heft): | 270 |
| Heft: | 16 |
| Seite(n): | 9638 |
| Seiten bis: | 9644 |
| Erscheinungsdatum: | 21-Apr-1995 |
| Verlag / Hrsg. Institution: | American Society for Biochemistry and Molecular Biology |
| ISSN: | 1083-351X |
| Sprache: | Englisch |
| Fachgebiet (DDC): | 572: Biochemie |
| Zusammenfassung: | Palmitoylation of p63, a type II membrane protein localized in the endoplasmic reticulum, is induced in a reversible manner by the drug brefeldin A. To study the requirements for palmitoylation, mutant forms of p63 were expressed in COS cells and analyzed by metabolic labeling with [3H]palmitate, immunoprecipitation, and SDS-polyacrylamide gel electrophoresis. By investigating deletion and point mutations, Cys100 in the 106-amino acid cytoplasmic tail of p63 has been identified as the site of acylation. Site-directed mutagenesis of residues 99-105 together with cytoplasmic tail truncation mutants showed that the amino acids surrounding Cys100 are not critical for palmitoylation of this residue. Analysis of a chimeric construct between p63 and the plasma membrane protein dipeptidylpeptidase IV further revealed that p63 palmitoylation is not dependent on its transmembrane domain. In contrast, the six-amino acid distance between the end of the predicted transmembrane domain and the palmitoylation site was found to be essential for proper acylation of p63. |
| URI: | https://digitalcollection.zhaw.ch/handle/11475/6802 |
| Volltext Version: | Publizierte Version |
| Lizenz (gemäss Verlagsvertrag): | Lizenz gemäss Verlagsvertrag |
| Departement: | Life Sciences und Facility Management |
| Enthalten in den Sammlungen: | Publikationen Life Sciences und Facility Management |
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Schweizer, A., Rohrer, J., & Kornfeld, S. (1995). Determination of the structural requirements for palmitoylation of p63. Journal of Biological Chemistry, 270(16), 9638–9644. https://doi.org/10.1074/jbc.270.16.9638
Schweizer, A., Rohrer, J. and Kornfeld, S. (1995) ‘Determination of the structural requirements for palmitoylation of p63’, Journal of Biological Chemistry, 270(16), pp. 9638–9644. Available at: https://doi.org/10.1074/jbc.270.16.9638.
A. Schweizer, J. Rohrer, and S. Kornfeld, “Determination of the structural requirements for palmitoylation of p63,” Journal of Biological Chemistry, vol. 270, no. 16, pp. 9638–9644, Apr. 1995, doi: 10.1074/jbc.270.16.9638.
SCHWEIZER, Anja, Jack ROHRER und Stuart KORNFELD, 1995. Determination of the structural requirements for palmitoylation of p63. Journal of Biological Chemistry. 21 April 1995. Bd. 270, Nr. 16, S. 9638–9644. DOI 10.1074/jbc.270.16.9638
Schweizer, Anja, Jack Rohrer, and Stuart Kornfeld. 1995. “Determination of the Structural Requirements for Palmitoylation of P63.” Journal of Biological Chemistry 270 (16): 9638–44. https://doi.org/10.1074/jbc.270.16.9638.
Schweizer, Anja, et al. “Determination of the Structural Requirements for Palmitoylation of P63.” Journal of Biological Chemistry, vol. 270, no. 16, Apr. 1995, pp. 9638–44, https://doi.org/10.1074/jbc.270.16.9638.
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