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dc.contributor.authorRohrer, Jack-
dc.contributor.authorKornfeld, Rosalind-
dc.date.accessioned2018-06-13T08:30:49Z-
dc.date.available2018-06-13T08:30:49Z-
dc.date.issued2001-06-
dc.identifier.issn1939-4586de_CH
dc.identifier.issn1059-1524de_CH
dc.identifier.urihttps://digitalcollection.zhaw.ch/handle/11475/6801-
dc.description.abstractA crucial step in lysosomal biogenesis is catalyzed by "uncovering" enzyme (UCE), which removes a covering N-acetylglucosamine from the mannose 6-phosphate (Man-6-P) recognition marker on lysosomal hydrolases. This study shows that UCE resides in the trans-Golgi network (TGN) and cycles between the TGN and plasma membrane. The cytosolic domain of UCE contains two potential endocytosis motifs: (488)YHPL and C-terminal (511)NPFKD. YHPL is shown to be the more potent of the two in retrieval of UCE from the plasma membrane. A green-fluorescent protein-UCE transmembrane-cytosolic domain fusion protein colocalizes with TGN 46, as does endogenous UCE in HeLa cells, showing that the transmembrane and cytosolic domains determine intracellular location. These data imply that the Man-6-P recognition marker is formed in the TGN, the compartment where Man-6-P receptors bind cargo and are packaged into clathrin-coated vesicles.de_CH
dc.language.isoende_CH
dc.publisherAmerican Society for Cell Biologyde_CH
dc.relation.ispartofMolecular Biology of the Cellde_CH
dc.rightsLicence according to publishing contractde_CH
dc.subject.ddc571: Physiologie und verwandte Themende_CH
dc.titleLysosomal hydrolase mannose 6-phosphate uncovering enzyme resides in the trans-Golgi networkde_CH
dc.typeBeitrag in wissenschaftlicher Zeitschriftde_CH
dcterms.typeTextde_CH
zhaw.departementLife Sciences und Facility Managementde_CH
zhaw.organisationalunitInstitut für Chemie und Biotechnologie (ICBT)de_CH
dc.identifier.doi10.1091/mbc.12.6.1623de_CH
zhaw.funding.euNode_CH
zhaw.issue6de_CH
zhaw.originated.zhawYesde_CH
zhaw.pages.end1631de_CH
zhaw.pages.start1623de_CH
zhaw.publication.statuspublishedVersionde_CH
zhaw.volume12de_CH
zhaw.publication.reviewPeer review (Publikation)de_CH
zhaw.webfeedZellphysiologiede_CH
Appears in collections:Publikationen Life Sciences und Facility Management

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Rohrer, J., & Kornfeld, R. (2001). Lysosomal hydrolase mannose 6-phosphate uncovering enzyme resides in the trans-Golgi network. Molecular Biology of the Cell, 12(6), 1623–1631. https://doi.org/10.1091/mbc.12.6.1623
Rohrer, J. and Kornfeld, R. (2001) ‘Lysosomal hydrolase mannose 6-phosphate uncovering enzyme resides in the trans-Golgi network’, Molecular Biology of the Cell, 12(6), pp. 1623–1631. Available at: https://doi.org/10.1091/mbc.12.6.1623.
J. Rohrer and R. Kornfeld, “Lysosomal hydrolase mannose 6-phosphate uncovering enzyme resides in the trans-Golgi network,” Molecular Biology of the Cell, vol. 12, no. 6, pp. 1623–1631, Jun. 2001, doi: 10.1091/mbc.12.6.1623.
ROHRER, Jack und Rosalind KORNFELD, 2001. Lysosomal hydrolase mannose 6-phosphate uncovering enzyme resides in the trans-Golgi network. Molecular Biology of the Cell. Juni 2001. Bd. 12, Nr. 6, S. 1623–1631. DOI 10.1091/mbc.12.6.1623
Rohrer, Jack, and Rosalind Kornfeld. 2001. “Lysosomal Hydrolase Mannose 6-Phosphate Uncovering Enzyme Resides in the Trans-Golgi Network.” Molecular Biology of the Cell 12 (6): 1623–31. https://doi.org/10.1091/mbc.12.6.1623.
Rohrer, Jack, and Rosalind Kornfeld. “Lysosomal Hydrolase Mannose 6-Phosphate Uncovering Enzyme Resides in the Trans-Golgi Network.” Molecular Biology of the Cell, vol. 12, no. 6, June 2001, pp. 1623–31, https://doi.org/10.1091/mbc.12.6.1623.


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