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dc.contributor.authorNair, Prashant-
dc.contributor.authorSchaub, Beat E-
dc.contributor.authorRohrer, Jack-
dc.date.accessioned2018-06-13T08:21:45Z-
dc.date.available2018-06-13T08:21:45Z-
dc.date.issued2003-07-04-
dc.identifier.issn1083-351Xde_CH
dc.identifier.urihttps://digitalcollection.zhaw.ch/handle/11475/6795-
dc.description.abstractIntracellular cycling of the cation-dependent mannose 6-phosphate receptor (CD-MPR) between different compartments is directed by signals localized in its cytoplasmic tail. A di-aromatic motif (Phe18-Trp19 with Trp19 as the key residue) in its cytoplasmic tail is required for the sorting of the receptor from late endosomes back to the Golgi apparatus. However, the cation-independent mannose 6-phosphate receptor (CI-MPR) lacks such a di-aromatic motif. Therefore the ability of amino acids other than aromatic residues to replace Trp19 in the CD-MPR cytoplasmic tail was tested. Mutant constructs with bulky hydrophobic residues (valine, isoleucine, or leucine) instead of Trp19 exhibited 30-60% decreases in binding to the tail interacting protein of 47 kDa (Tip47), a protein mediating this transport step, and partially prevented receptor delivery to lysosomes. Decreasing hydrophobicity of residues at position 19 resulted in further impairment of Tip47 binding and an increase of receptor accumulation in lysosomes. Intriguingly, mutants mislocalized to lysosomes did not completely co-localize with a lysosomal membrane protein, which might suggest the presence of subdomains within lysosomes. These data indicate that sorting of the CD-MPR in late endosomes requires a distinct di-aromatic motif with only limited possibilities for variations, in contrast to the CI-MPR, which seems to require a putative loop (Pro49-Pro-Ala-Pro-Arg-Pro-Gly55) along with additional hydrophobic residues in the cytoplasmic tail. This raises the possibility of two separate binding sites on Tip47 because both receptors require binding to Tip47 for endosomal sorting.de_CH
dc.language.isoende_CH
dc.publisherAmerican Society for Biochemistry and Molecular Biologyde_CH
dc.relation.ispartofJournal of Biological Chemistryde_CH
dc.rightsLicence according to publishing contractde_CH
dc.subject.ddc571: Physiologie und verwandte Themende_CH
dc.subject.ddc572: Biochemiede_CH
dc.titleCharacterization of the endosomal sorting signal of the cation-dependent mannose 6-phosphate receptorde_CH
dc.typeBeitrag in wissenschaftlicher Zeitschriftde_CH
dcterms.typeTextde_CH
zhaw.departementLife Sciences und Facility Managementde_CH
zhaw.organisationalunitInstitut für Chemie und Biotechnologie (ICBT)de_CH
dc.identifier.doi10.1074/jbc.M300174200de_CH
zhaw.funding.euNode_CH
zhaw.issue27de_CH
zhaw.originated.zhawYesde_CH
zhaw.pages.end24758de_CH
zhaw.pages.start24753de_CH
zhaw.publication.statuspublishedVersionde_CH
zhaw.volume278de_CH
zhaw.publication.reviewPeer review (Publikation)de_CH
zhaw.webfeedZellphysiologiede_CH
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Nair, P., Schaub, B. E., & Rohrer, J. (2003). Characterization of the endosomal sorting signal of the cation-dependent mannose 6-phosphate receptor. Journal of Biological Chemistry, 278(27), 24753–24758. https://doi.org/10.1074/jbc.M300174200
Nair, P., Schaub, B.E. and Rohrer, J. (2003) ‘Characterization of the endosomal sorting signal of the cation-dependent mannose 6-phosphate receptor’, Journal of Biological Chemistry, 278(27), pp. 24753–24758. Available at: https://doi.org/10.1074/jbc.M300174200.
P. Nair, B. E. Schaub, and J. Rohrer, “Characterization of the endosomal sorting signal of the cation-dependent mannose 6-phosphate receptor,” Journal of Biological Chemistry, vol. 278, no. 27, pp. 24753–24758, Jul. 2003, doi: 10.1074/jbc.M300174200.
NAIR, Prashant, Beat E SCHAUB und Jack ROHRER, 2003. Characterization of the endosomal sorting signal of the cation-dependent mannose 6-phosphate receptor. Journal of Biological Chemistry. 4 Juli 2003. Bd. 278, Nr. 27, S. 24753–24758. DOI 10.1074/jbc.M300174200
Nair, Prashant, Beat E Schaub, and Jack Rohrer. 2003. “Characterization of the Endosomal Sorting Signal of the Cation-Dependent Mannose 6-Phosphate Receptor.” Journal of Biological Chemistry 278 (27): 24753–58. https://doi.org/10.1074/jbc.M300174200.
Nair, Prashant, et al. “Characterization of the Endosomal Sorting Signal of the Cation-Dependent Mannose 6-Phosphate Receptor.” Journal of Biological Chemistry, vol. 278, no. 27, July 2003, pp. 24753–58, https://doi.org/10.1074/jbc.M300174200.


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