1. ZHAW digitalcollection
  2. Life Sciences und Facility Management
  3. Publikationen
  4. Publikationen Life Sciences und Facility Management
Please use this identifier to cite or link to this item: https://doi.org/10.21256/zhaw-1749
Full metadata record
DC FieldValueLanguage
dc.contributor.authorRimann, Markus-
dc.contributor.authorBono, Epifania-
dc.contributor.authorAnnaheim, Helene-
dc.contributor.authorBleisch, Matthias-
dc.contributor.authorGraf-Hausner, Ursula-
dc.date.accessioned2018-02-27T15:34:28Z-
dc.date.available2018-02-27T15:34:28Z-
dc.date.issued2015-
dc.identifier.issn2472-6303de_CH
dc.identifier.issn2472-6311de_CH
dc.identifier.urihttps://digitalcollection.zhaw.ch/handle/11475/3280-
dc.description.abstractCells grown in 3D are more physiologically relevant than cells cultured in 2D. To use 3D models in substance testing and regenerative medicine, reproducibility and standardization are important. Bioprinting offers not only automated standardizable processes but also the production of complex tissue-like structures in an additive manner. We developed an all-in-one bioprinting solution to produce soft tissue models. The holistic approach included (1) a bioprinter in a sterile environment, (2) a light-induced bioink polymerization unit, (3) a user-friendly software, (4) the capability to print in standard labware for high-throughput screening, (5) cell-compatible inkjet-based printheads, (6) a cell-compatible ready-to-use BioInk, and (7) standard operating procedures. In a proof-of-concept study, skin as a reference soft tissue model was printed. To produce dermal equivalents, primary human dermal fibroblasts were printed in alternating layers with BioInk and cultured for up to 7 weeks. During long-term cultures, the models were remodeled and fully populated with viable and spreaded fibroblasts. Primary human dermal keratinocytes were seeded on top of dermal equivalents, and epidermis-like structures were formed as verified with hematoxylin and eosin staining and immunostaining. However, a fully stratified epidermis was not achieved. Nevertheless, this is one of the first reports of an integrative bioprinting strategy for industrial routine application.de_CH
dc.language.isoende_CH
dc.publisherSagede_CH
dc.relation.ispartofSLAS Technology: Translating Life Sciences Innovationde_CH
dc.rightsLicence according to publishing contractde_CH
dc.subjectSoft tissue modelsde_CH
dc.subject3D cell culturede_CH
dc.subjectBioprintingde_CH
dc.subjectAutomation, standardizationde_CH
dc.subject.ddc610: Medizin und Gesundheitde_CH
dc.titleStandardized 3D bioprinting of soft tissue models with human primary cellsde_CH
dc.typeBeitrag in wissenschaftlicher Zeitschriftde_CH
dcterms.typeTextde_CH
zhaw.departementLife Sciences und Facility Managementde_CH
zhaw.publisher.placeThousand Oaksde_CH
dc.identifier.doi10.21256/zhaw-1749-
dc.identifier.doi10.1177/2211068214567146de_CH
zhaw.funding.euNode_CH
zhaw.issue4de_CH
zhaw.originated.zhawYesde_CH
zhaw.pages.end509de_CH
zhaw.pages.start496de_CH
zhaw.publication.statuspublishedVersionde_CH
zhaw.volume21de_CH
zhaw.publication.reviewPeer review (Publikation)de_CH
Appears in collections:Publikationen Life Sciences und Facility Management

Files in This Item:
File Description SizeFormat 
2016_Rimann_Standardized_3D_Bioprinting_JournalLaboratoryAutomation.pdf652.28 kBAdobe PDFThumbnail
View/Open
Show simple item record
Rimann, M., Bono, E., Annaheim, H., Bleisch, M., & Graf-Hausner, U. (2015). Standardized 3D bioprinting of soft tissue models with human primary cells. SLAS Technology: Translating Life Sciences Innovation, 21(4), 496–509. https://doi.org/10.21256/zhaw-1749
Rimann, M. et al. (2015) ‘Standardized 3D bioprinting of soft tissue models with human primary cells’, SLAS Technology: Translating Life Sciences Innovation, 21(4), pp. 496–509. Available at: https://doi.org/10.21256/zhaw-1749.
M. Rimann, E. Bono, H. Annaheim, M. Bleisch, and U. Graf-Hausner, “Standardized 3D bioprinting of soft tissue models with human primary cells,” SLAS Technology: Translating Life Sciences Innovation, vol. 21, no. 4, pp. 496–509, 2015, doi: 10.21256/zhaw-1749.
RIMANN, Markus, Epifania BONO, Helene ANNAHEIM, Matthias BLEISCH und Ursula GRAF-HAUSNER, 2015. Standardized 3D bioprinting of soft tissue models with human primary cells. SLAS Technology: Translating Life Sciences Innovation. 2015. Bd. 21, Nr. 4, S. 496–509. DOI 10.21256/zhaw-1749
Rimann, Markus, Epifania Bono, Helene Annaheim, Matthias Bleisch, and Ursula Graf-Hausner. 2015. “Standardized 3D Bioprinting of Soft Tissue Models with Human Primary Cells.” SLAS Technology: Translating Life Sciences Innovation 21 (4): 496–509. https://doi.org/10.21256/zhaw-1749.
Rimann, Markus, et al. “Standardized 3D Bioprinting of Soft Tissue Models with Human Primary Cells.” SLAS Technology: Translating Life Sciences Innovation, vol. 21, no. 4, 2015, pp. 496–509, https://doi.org/10.21256/zhaw-1749.


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.