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Please use this identifier to cite or link to this item: https://doi.org/10.21256/zhaw-30490
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dc.contributor.authorOtt, Vivian-
dc.contributor.authorOtt, Jan-
dc.contributor.authorEibl, Dieter-
dc.contributor.authorEibl-Schindler, Regine-
dc.date.accessioned2024-04-22T14:40:37Z-
dc.date.available2024-04-22T14:40:37Z-
dc.date.issued2024-04-17-
dc.identifier.issn2227-9717de_CH
dc.identifier.urihttps://digitalcollection.zhaw.ch/handle/11475/30490-
dc.description.abstractModern production processes for biopharmaceuticals often work with very high cell densities. Moreover, there is a trend towards moving from fed-batch to continuous perfusion processes; a development that is influencing the requirements for bioreactor design and process control. In this study, the transfer of fed-batch and perfusion experiments between different cylindrical stirred lab-scale bioreactors and Thermo Scientific’sTM (Waltham, MA, USA) cubical HyPerformaTM DynaDriveTM Single-Use Bioreactor was investigated. Different scaling parameters were used, which were selected based on the requirements of the respective processes. Peak cell densities of up to 49 × 106 cells mL−1 and antibody titers of up to 5.2 g L−1 were achieved in 15- to 16-day fed-batch experiments. In 50-day perfusion cultivations, a viable cell volume of >100 mm3 mL−1 was maintained and more than 1 g L−1 d−1 of antibodies were harvested. The perfusion processes were automated with both cell bleed control and glucose concentration control. Cell retention was performed using Repligen’s (Waltham, MA, USA) XCell® ATF perfusion systems and single-use devices. In summary, approaches for successfully scaling highly productive fed-batch and perfusion processes between geometrically dissimilar lab and pilot scale bioreactors were demonstrated. The advantages of perfusion in comparison to fed-batch processes were also observed.de_CH
dc.language.isoende_CH
dc.publisherMDPIde_CH
dc.relation.ispartofProcessesde_CH
dc.rightshttp://creativecommons.org/licenses/by/4.0/de_CH
dc.subjectAutomationde_CH
dc.subjectChinese hamster ovary cellsde_CH
dc.subjectMonoclonal antibodiesde_CH
dc.subjectUpstream processde_CH
dc.subjectProcess intensificationde_CH
dc.subjectPerfusionde_CH
dc.subject.ddc660: Technische Chemiede_CH
dc.titleScaling fed-batch and perfusion antibody production processes in geometrically dissimilar stirred bioreactorsde_CH
dc.typeBeitrag in wissenschaftlicher Zeitschriftde_CH
dcterms.typeTextde_CH
zhaw.departementLife Sciences und Facility Managementde_CH
zhaw.organisationalunitInstitut für Chemie und Biotechnologie (ICBT)de_CH
dc.identifier.doi10.3390/pr12040806de_CH
dc.identifier.doi10.21256/zhaw-30490-
zhaw.funding.euNode_CH
zhaw.issue4de_CH
zhaw.originated.zhawYesde_CH
zhaw.pages.start806de_CH
zhaw.publication.statuspublishedVersionde_CH
zhaw.volume12de_CH
zhaw.publication.reviewPeer review (Publikation)de_CH
zhaw.author.additionalNode_CH
zhaw.display.portraitYesde_CH
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Ott, V., Ott, J., Eibl, D., & Eibl-Schindler, R. (2024). Scaling fed-batch and perfusion antibody production processes in geometrically dissimilar stirred bioreactors. Processes, 12(4), 806. https://doi.org/10.3390/pr12040806
Ott, V. et al. (2024) ‘Scaling fed-batch and perfusion antibody production processes in geometrically dissimilar stirred bioreactors’, Processes, 12(4), p. 806. Available at: https://doi.org/10.3390/pr12040806.
V. Ott, J. Ott, D. Eibl, and R. Eibl-Schindler, “Scaling fed-batch and perfusion antibody production processes in geometrically dissimilar stirred bioreactors,” Processes, vol. 12, no. 4, p. 806, Apr. 2024, doi: 10.3390/pr12040806.
OTT, Vivian, Jan OTT, Dieter EIBL und Regine EIBL-SCHINDLER, 2024. Scaling fed-batch and perfusion antibody production processes in geometrically dissimilar stirred bioreactors. Processes. 17 April 2024. Bd. 12, Nr. 4, S. 806. DOI 10.3390/pr12040806
Ott, Vivian, Jan Ott, Dieter Eibl, and Regine Eibl-Schindler. 2024. “Scaling Fed-Batch and Perfusion Antibody Production Processes in Geometrically Dissimilar Stirred Bioreactors.” Processes 12 (4): 806. https://doi.org/10.3390/pr12040806.
Ott, Vivian, et al. “Scaling Fed-Batch and Perfusion Antibody Production Processes in Geometrically Dissimilar Stirred Bioreactors.” Processes, vol. 12, no. 4, Apr. 2024, p. 806, https://doi.org/10.3390/pr12040806.


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