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Please use this identifier to cite or link to this item: https://doi.org/10.21256/zhaw-29951
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dc.contributor.authorRaguz Nakic, Zrinka-
dc.contributor.authorPeters, Christin-
dc.date.accessioned2024-02-15T14:40:13Z-
dc.date.available2024-02-15T14:40:13Z-
dc.date.issued2023-06-28-
dc.identifier.issn0009-4293de_CH
dc.identifier.urihttps://digitalcollection.zhaw.ch/handle/11475/29951-
dc.description.abstractPreparation of expression vectors using conventional cloning strategies is laborious and not suitable for the design of metabolic pathways or enzyme cascades, which usually requires the preparation of a vector library to identify productive clones. Recently, Modular Cloning as a novel cloning technique in synthetic biology has been developed. Modular Cloning relies on Golden Gate assembly and supports preparation of individual expression vectors in one-step and one-pot reactions, thus allowing rapid generation of vector libraries. A number of Modular Cloning toolkits for specific applications has been established, providing a collection of distinct genetic elements such as promoters, ribosome binding sites and tags, that can be combined individually in one-step using defined fusion sites. Modular Cloning has been successfully applied to generate various strains for producing value-added compounds. This was achieved by orchestrating complex pathways involving up to 20 enzymes. Due to the novelty of the genetic approach, industrial applications are still rare. In addition, some applications are limited due to the lack of high-throughput screening methods. This shifts the bottleneck from library preparation to screening capacity and needs to be addressed by future developments to pave the path for the establishment of Modular Cloning in industrial applications.de_CH
dc.language.isoende_CH
dc.publisherSwiss Chemical Societyde_CH
dc.relation.ispartofChimiade_CH
dc.rightshttp://creativecommons.org/licenses/by/4.0/de_CH
dc.subjectExpression controlde_CH
dc.subjectGolden Gate assemblyde_CH
dc.subjectModular cloningde_CH
dc.subjectPathway designde_CH
dc.subjectBinding sitesde_CH
dc.subjectPromoter regions, geneticde_CH
dc.subjectCloning, molecularde_CH
dc.subjectHigh-throughput screening assayde_CH
dc.subjectSynthetic biologyde_CH
dc.subject.ddc572: Biochemiede_CH
dc.subject.ddc660.6: Biotechnologiede_CH
dc.titleModular cloning by Golden Gate assembly and possible application in pathway designde_CH
dc.typeBeitrag in wissenschaftlicher Zeitschriftde_CH
dcterms.typeTextde_CH
zhaw.departementLife Sciences und Facility Managementde_CH
zhaw.organisationalunitInstitut für Chemie und Biotechnologie (ICBT)de_CH
dc.identifier.doi10.2533/chimia.2023.437de_CH
dc.identifier.doi10.21256/zhaw-29951-
dc.identifier.pmid38047784de_CH
zhaw.funding.euNode_CH
zhaw.issue6de_CH
zhaw.originated.zhawYesde_CH
zhaw.pages.end441de_CH
zhaw.pages.start437de_CH
zhaw.publication.statuspublishedVersionde_CH
zhaw.volume77de_CH
zhaw.publication.reviewPeer review (Publikation)de_CH
zhaw.webfeedBiokatalysede_CH
zhaw.funding.zhawPlasmid-Toolbox für Enzymkaskadende_CH
zhaw.author.additionalNode_CH
zhaw.display.portraitYesde_CH
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Raguz Nakic, Z., & Peters, C. (2023). Modular cloning by Golden Gate assembly and possible application in pathway design. Chimia, 77(6), 437–441. https://doi.org/10.2533/chimia.2023.437
Raguz Nakic, Z. and Peters, C. (2023) ‘Modular cloning by Golden Gate assembly and possible application in pathway design’, Chimia, 77(6), pp. 437–441. Available at: https://doi.org/10.2533/chimia.2023.437.
Z. Raguz Nakic and C. Peters, “Modular cloning by Golden Gate assembly and possible application in pathway design,” Chimia, vol. 77, no. 6, pp. 437–441, Jun. 2023, doi: 10.2533/chimia.2023.437.
RAGUZ NAKIC, Zrinka und Christin PETERS, 2023. Modular cloning by Golden Gate assembly and possible application in pathway design. Chimia. 28 Juni 2023. Bd. 77, Nr. 6, S. 437–441. DOI 10.2533/chimia.2023.437
Raguz Nakic, Zrinka, and Christin Peters. 2023. “Modular Cloning by Golden Gate Assembly and Possible Application in Pathway Design.” Chimia 77 (6): 437–41. https://doi.org/10.2533/chimia.2023.437.
Raguz Nakic, Zrinka, and Christin Peters. “Modular Cloning by Golden Gate Assembly and Possible Application in Pathway Design.” Chimia, vol. 77, no. 6, June 2023, pp. 437–41, https://doi.org/10.2533/chimia.2023.437.


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