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Please use this identifier to cite or link to this item: https://doi.org/10.21256/zhaw-1685
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dc.contributor.authorMichel, Christian-
dc.contributor.authorHerzog, Simon-
dc.contributor.authorde Capitani, Christian-
dc.contributor.authorBurkhardt-Holm, Patricia-
dc.contributor.authorPietsch, Constanze-
dc.date.accessioned2018-02-15T15:45:03Z-
dc.date.available2018-02-15T15:45:03Z-
dc.date.issued2014-
dc.identifier.issn1932-6203de_CH
dc.identifier.urihttps://digitalcollection.zhaw.ch/handle/11475/2849-
dc.description.abstractWorldwide increases in fluvial fine sediment are a threat to aquatic animal health. Fluvial fine sediment is always a mixture of particles whose mineralogical composition differs depending on the sediment source and catchment area geology. Nonetheless, whether particle impact in aquatic organisms differs between mineral species remains to be investigated. This study applied an in vitro approach to evaluate cytotoxicity and uptake of four common fluvial mineral particles (quartz, feldspar, mica, and kaolin; concentrations: 10, 50, 250 mg L(-1)) in the rainbow trout epithelial gill cell line RTgill-W1. Cells were exposed for 24, 48, 72, and 96 h. Cytotoxicity assays for cell membrane integrity (propidium iodide assay), oxidative stress (H2DCF-DA assay), and metabolic activity (MTT assay) were applied. These assays were complemented with cell counts and transmission electron microscopy. Regardless of mineral species, particles ≤ 2 µm in diameter were taken up by the cells, suggesting that particles of all mineral species came into contact and interacted with the cells. Not all particles, however, caused strong cytotoxicity: Among all assays the tectosilicates quartz and feldspar caused sporadic maximum changes of 0.8-1.2-fold compared to controls. In contrast, cytotoxicity of the clay particles was distinctly stronger and even differed between the two particle types: mica induced concentration-dependent increases in free radicals, with consistent 1.6-1.8-fold-changes at the 250 mg L(-1) concentration, and a dilated endoplasmic reticulum. Kaolin caused concentration-dependent increases in cell membrane damage, with consistent 1.3-1.6-fold increases at the 250 mg L(-1) concentration. All effects occurred in the presence or absence of 10% fetal bovine serum. Cell numbers per se were marginally affected. Results indicate that (i.) natural mineral particles can be cytotoxic to gill epithelial cells, (ii.) their cytotoxic potential differs between mineral species, with clay particles being more cytotoxic, and (iii.) some clays might induce effects comparable to engineered nanoparticles.de_CH
dc.language.isoende_CH
dc.publisherPublic Library of Sciencede_CH
dc.relation.ispartofPLOS ONEde_CH
dc.rightsLicence according to publishing contractde_CH
dc.subjectAnimalsde_CH
dc.subjectCell Countde_CH
dc.subjectCell Survivalde_CH
dc.subjectCells, Culturedde_CH
dc.subjectEpithelial Cellsde_CH
dc.subjectGillsde_CH
dc.subjectMineralsde_CH
dc.subjectOncorhynchus mykissde_CH
dc.subject.ddc571: Physiologie und verwandte Themende_CH
dc.subject.ddc630: Landwirtschaftde_CH
dc.titleNatural mineral particles are cytotoxic to rainbow trout gill epithelial cells In vitrode_CH
dc.typeBeitrag in wissenschaftlicher Zeitschriftde_CH
dcterms.typeTextde_CH
zhaw.departementLife Sciences und Facility Managementde_CH
zhaw.organisationalunitInstitut für Umwelt und Natürliche Ressourcen (IUNR)de_CH
dc.identifier.doi10.21256/zhaw-1685-
dc.identifier.doi10.1371/journal.pone.0100856de_CH
dc.identifier.pmid24991818de_CH
zhaw.funding.euNode_CH
zhaw.issue7de_CH
zhaw.originated.zhawYesde_CH
zhaw.publication.statuspublishedVersionde_CH
zhaw.volume9de_CH
zhaw.publication.reviewPeer review (Publikation)de_CH
zhaw.webfeedAquakultur-Systemede_CH
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Michel, C., Herzog, S., de Capitani, C., Burkhardt-Holm, P., & Pietsch, C. (2014). Natural mineral particles are cytotoxic to rainbow trout gill epithelial cells In vitro. Plos One, 9(7). https://doi.org/10.21256/zhaw-1685
Michel, C. et al. (2014) ‘Natural mineral particles are cytotoxic to rainbow trout gill epithelial cells In vitro’, PLOS ONE, 9(7). Available at: https://doi.org/10.21256/zhaw-1685.
C. Michel, S. Herzog, C. de Capitani, P. Burkhardt-Holm, and C. Pietsch, “Natural mineral particles are cytotoxic to rainbow trout gill epithelial cells In vitro,” PLOS ONE, vol. 9, no. 7, 2014, doi: 10.21256/zhaw-1685.
MICHEL, Christian, Simon HERZOG, Christian DE CAPITANI, Patricia BURKHARDT-HOLM und Constanze PIETSCH, 2014. Natural mineral particles are cytotoxic to rainbow trout gill epithelial cells In vitro. PLOS ONE. 2014. Bd. 9, Nr. 7. DOI 10.21256/zhaw-1685
Michel, Christian, Simon Herzog, Christian de Capitani, Patricia Burkhardt-Holm, and Constanze Pietsch. 2014. “Natural Mineral Particles Are Cytotoxic to Rainbow Trout Gill Epithelial Cells in Vitro.” Plos One 9 (7). https://doi.org/10.21256/zhaw-1685.
Michel, Christian, et al. “Natural Mineral Particles Are Cytotoxic to Rainbow Trout Gill Epithelial Cells in Vitro.” Plos One, vol. 9, no. 7, 2014, https://doi.org/10.21256/zhaw-1685.


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