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Please use this identifier to cite or link to this item: https://doi.org/10.21256/zhaw-1670
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dc.contributor.authorSchulz, Nadine-
dc.contributor.authorChaachouay, Hassan-
dc.contributor.authorNytko, Katarzyna J.-
dc.contributor.authorWeyland, Mathias S.-
dc.contributor.authorRoos, Malgorzata-
dc.contributor.authorFüchslin, Rudolf Marcel-
dc.contributor.authorGuscetti, Franco-
dc.contributor.authorScheidegger, Stephan-
dc.contributor.authorRohrer Bley, Carla-
dc.date.accessioned2018-02-14T10:54:17Z-
dc.date.available2018-02-14T10:54:17Z-
dc.date.issued2017-
dc.identifier.issn1422-0067de_CH
dc.identifier.issn1661-6596de_CH
dc.identifier.urihttps://digitalcollection.zhaw.ch/handle/11475/2787-
dc.description.abstractTime resolved data of DNA damage and repair after radiotherapy elucidates the relation between damage, repair, and cell survival. While well characterized in vitro, little is known about the time-course of DNA damage response in tumors sampled from individual patients. Kinetics of DNA damage after radiotherapy was assessed in eight dogs using repeated in vivo samples of tumor and co-irradiated normal tissue analyzed with comet assay and phosphorylated H2AX (γH2AX) immunohistochemistry. In vivo results were then compared (in silico) with a dynamic mathematical model for DNA damage formation and repair. Maximum %DNA in tail was observed at 15-60 min after irradiation, with a rapid decrease. Time-courses of γH2AX-foci paralleled these findings with a small time delay and were not influenced by covariates. The evolutionary parameter search based on %DNA in tail revealed a good fit of the DNA repair model to in vivo data for pooled sarcoma time-courses, but fits for individual sarcoma time-courses suffer from the heterogeneous nature of the in vivo data. It was possible to follow dynamics of comet tail intensity and γH2AX-foci during a course of radiation using a minimally invasive approach. DNA repair can be quantitatively investigated as time-courses of individual patients by integrating this resulting data into a dynamic mathematical model.de_CH
dc.language.isoende_CH
dc.publisherMDPIde_CH
dc.relation.ispartofInternational Journal of Molecular Sciencesde_CH
dc.rightshttp://creativecommons.org/licenses/by/4.0/de_CH
dc.subjectComet assayde_CH
dc.subjectRepair kineticsde_CH
dc.subjectRepair pathway modelde_CH
dc.subject.ddc572: Biochemiede_CH
dc.titleDynamic in vivo profiling of DNA damage and repair after radiotherapy using canine patients as a modelde_CH
dc.typeBeitrag in wissenschaftlicher Zeitschriftde_CH
dcterms.typeTextde_CH
zhaw.departementSchool of Engineeringde_CH
zhaw.organisationalunitInstitut für Angewandte Mathematik und Physik (IAMP)de_CH
dc.identifier.doi10.21256/zhaw-1670-
dc.identifier.doi10.3390/ijms18061176de_CH
zhaw.funding.euNode_CH
zhaw.issue6/1176de_CH
zhaw.originated.zhawYesde_CH
zhaw.publication.statuspublishedVersionde_CH
zhaw.volume18de_CH
zhaw.publication.reviewPeer review (Publikation)de_CH
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Schulz, N., Chaachouay, H., Nytko, K. J., Weyland, M. S., Roos, M., Füchslin, R. M., Guscetti, F., Scheidegger, S., & Rohrer Bley, C. (2017). Dynamic in vivo profiling of DNA damage and repair after radiotherapy using canine patients as a model. International Journal of Molecular Sciences, 18(6/1176). https://doi.org/10.21256/zhaw-1670
Schulz, N. et al. (2017) ‘Dynamic in vivo profiling of DNA damage and repair after radiotherapy using canine patients as a model’, International Journal of Molecular Sciences, 18(6/1176). Available at: https://doi.org/10.21256/zhaw-1670.
N. Schulz et al., “Dynamic in vivo profiling of DNA damage and repair after radiotherapy using canine patients as a model,” International Journal of Molecular Sciences, vol. 18, no. 6/1176, 2017, doi: 10.21256/zhaw-1670.
SCHULZ, Nadine, Hassan CHAACHOUAY, Katarzyna J. NYTKO, Mathias S. WEYLAND, Malgorzata ROOS, Rudolf Marcel FÜCHSLIN, Franco GUSCETTI, Stephan SCHEIDEGGER und Carla ROHRER BLEY, 2017. Dynamic in vivo profiling of DNA damage and repair after radiotherapy using canine patients as a model. International Journal of Molecular Sciences. 2017. Bd. 18, Nr. 6/1176. DOI 10.21256/zhaw-1670
Schulz, Nadine, Hassan Chaachouay, Katarzyna J. Nytko, Mathias S. Weyland, Malgorzata Roos, Rudolf Marcel Füchslin, Franco Guscetti, Stephan Scheidegger, and Carla Rohrer Bley. 2017. “Dynamic in Vivo Profiling of DNA Damage and Repair after Radiotherapy Using Canine Patients as a Model.” International Journal of Molecular Sciences 18 (6/1176). https://doi.org/10.21256/zhaw-1670.
Schulz, Nadine, et al. “Dynamic in Vivo Profiling of DNA Damage and Repair after Radiotherapy Using Canine Patients as a Model.” International Journal of Molecular Sciences, vol. 18, no. 6/1176, 2017, https://doi.org/10.21256/zhaw-1670.


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