1. ZHAW digitalcollection
  2. Life Sciences und Facility Management
  3. Publikationen
  4. Publikationen Life Sciences und Facility Management
Full metadata record
DC FieldValueLanguage
dc.contributor.authorTsiapalis, Dimitrios-
dc.contributor.authorDe Pieri, Andrea-
dc.contributor.authorSpanoudes, Kyriakos-
dc.contributor.authorSallent, Ignacio-
dc.contributor.authorKearns, Stephen-
dc.contributor.authorKelly, Jack L.-
dc.contributor.authorRaghunath, Michael-
dc.contributor.authorZeugolis, Dimitrios I.-
dc.date.accessioned2023-02-24T13:26:58Z-
dc.date.available2023-02-24T13:26:58Z-
dc.date.issued2020-
dc.identifier.issn1758-5082de_CH
dc.identifier.issn1758-5090de_CH
dc.identifier.urihttps://digitalcollection.zhaw.ch/handle/11475/27134-
dc.description.abstractCellular therapies play an important role in tendon tissue engineering, with tenocytes being the most prominent and potent cell population available. However, for the development of a rich extracellular matrix tenocyte-assembled tendon equivalent, prolonged in vitro culture is required, which is associated with phenotypic drift. Recapitulation of tendon tissue microenvironment in vitro with cues that enhance and accelerate extracellular matrix synthesis and deposition, whilst maintaining tenocyte phenotype, may lead to functional cell therapies. Herein, we assessed the synergistic effect of low oxygen tension (enhances extracellular matrix synthesis) and macromolecular crowding (enhances extracellular matrix deposition) in human tenocyte culture. Protein analysis demonstrated that human tenocytes at 2% oxygen tension and with 50 μg ml-1 carrageenan (macromolecular crowder used) significantly increased synthesis and deposition of collagen types I, III, V and VI. Gene analysis at day 7 illustrated that human tenocytes at 2% oxygen tension and with 50 μg ml-1 carrageenan significantly increased the expression of prolyl 4-hydroxylase subunit alpha 1, procollagen-lysine 2- oxoglutarate 5-dioxygenase 2, scleraxis, tenomodulin and elastin, whilst chondrogenic (e.g. runt-related transcription factor 2, cartilage oligomeric matrix protein, aggrecan) and osteogenic (e.g. secreted phosphoprotein 1, bone gamma-carboxyglutamate protein) trans-differentiation markers were significantly down-regulated or remained unchanged. Collectively, our data clearly illustrates the beneficial synergistic effect of low oxygen tension and macromolecular crowding in the accelerated development of tissue equivalents.de_CH
dc.language.isoende_CH
dc.publisherIOP Publishingde_CH
dc.relation.ispartofBiofabricationde_CH
dc.rightsLicence according to publishing contractde_CH
dc.subjectAggrecande_CH
dc.subjectCarrageenande_CH
dc.subjectCell, ulturedde_CH
dc.subjectCollagen type Ide_CH
dc.subjectCollagen type IIIde_CH
dc.subjectDown-regulationde_CH
dc.subjectExtracellular matrixde_CH
dc.subjectExtracellular matrix proteinde_CH
dc.subjectHumansde_CH
dc.subjectMalede_CH
dc.subjectMiddle agedde_CH
dc.subjectOxygende_CH
dc.subjectProcollagen-proline dioxygenasede_CH
dc.subjectTendonde_CH
dc.subjectUp-regulationde_CH
dc.subject.ddc610.28: Biomedizin, Biomedizinische Technikde_CH
dc.titleThe synergistic effect of low oxygen tension and macromolecular crowding in the development of extracellular matrix-rich tendon equivalentsde_CH
dc.typeBeitrag in wissenschaftlicher Zeitschriftde_CH
dcterms.typeTextde_CH
zhaw.departementLife Sciences und Facility Managementde_CH
zhaw.organisationalunitInstitut für Chemie und Biotechnologie (ICBT)de_CH
dc.identifier.doi10.1088/1758-5090/ab6412de_CH
dc.identifier.pmid31855856de_CH
zhaw.funding.euNode_CH
zhaw.issue2de_CH
zhaw.originated.zhawYesde_CH
zhaw.pages.start25018de_CH
zhaw.publication.statuspublishedVersionde_CH
zhaw.volume12de_CH
zhaw.publication.reviewPeer review (Publikation)de_CH
zhaw.webfeedMetabolic Tissue Engineeringde_CH
zhaw.author.additionalNode_CH
zhaw.display.portraitYesde_CH
Appears in collections:Publikationen Life Sciences und Facility Management

Files in This Item:
There are no files associated with this item.
Show simple item record
Tsiapalis, D., De Pieri, A., Spanoudes, K., Sallent, I., Kearns, S., Kelly, J. L., Raghunath, M., & Zeugolis, D. I. (2020). The synergistic effect of low oxygen tension and macromolecular crowding in the development of extracellular matrix-rich tendon equivalents. Biofabrication, 12(2), 25018. https://doi.org/10.1088/1758-5090/ab6412
Tsiapalis, D. et al. (2020) ‘The synergistic effect of low oxygen tension and macromolecular crowding in the development of extracellular matrix-rich tendon equivalents’, Biofabrication, 12(2), p. 25018. Available at: https://doi.org/10.1088/1758-5090/ab6412.
D. Tsiapalis et al., “The synergistic effect of low oxygen tension and macromolecular crowding in the development of extracellular matrix-rich tendon equivalents,” Biofabrication, vol. 12, no. 2, p. 25018, 2020, doi: 10.1088/1758-5090/ab6412.
TSIAPALIS, Dimitrios, Andrea DE PIERI, Kyriakos SPANOUDES, Ignacio SALLENT, Stephen KEARNS, Jack L. KELLY, Michael RAGHUNATH und Dimitrios I. ZEUGOLIS, 2020. The synergistic effect of low oxygen tension and macromolecular crowding in the development of extracellular matrix-rich tendon equivalents. Biofabrication. 2020. Bd. 12, Nr. 2, S. 25018. DOI 10.1088/1758-5090/ab6412
Tsiapalis, Dimitrios, Andrea De Pieri, Kyriakos Spanoudes, Ignacio Sallent, Stephen Kearns, Jack L. Kelly, Michael Raghunath, and Dimitrios I. Zeugolis. 2020. “The Synergistic Effect of Low Oxygen Tension and Macromolecular Crowding in the Development of Extracellular Matrix-Rich Tendon Equivalents.” Biofabrication 12 (2): 25018. https://doi.org/10.1088/1758-5090/ab6412.
Tsiapalis, Dimitrios, et al. “The Synergistic Effect of Low Oxygen Tension and Macromolecular Crowding in the Development of Extracellular Matrix-Rich Tendon Equivalents.” Biofabrication, vol. 12, no. 2, 2020, p. 25018, https://doi.org/10.1088/1758-5090/ab6412.


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.