1. ZHAW digitalcollection
  2. Life Sciences und Facility Management
  3. Publikationen
  4. Publikationen Life Sciences und Facility Management
Full metadata record
DC FieldValueLanguage
dc.contributor.authorHeeb, Norbert V.-
dc.contributor.authorSchalles, Simone-
dc.contributor.authorLehner, Sandro-
dc.contributor.authorSchinkel, Lena-
dc.contributor.authorSchilling, Iris-
dc.contributor.authorLienemann, Peter-
dc.contributor.authorBogdal, Christian-
dc.contributor.authorKohler, Hans-Peter E.-
dc.date.accessioned2023-02-06T15:09:43Z-
dc.date.available2023-02-06T15:09:43Z-
dc.date.issued2019-07-
dc.identifier.issn0045-6535de_CH
dc.identifier.issn1879-1298de_CH
dc.identifier.urihttps://digitalcollection.zhaw.ch/handle/11475/26806-
dc.description.abstractShort-chain chlorinated paraffins (SCCPs) are polyhalogenated hydrocarbons as are hexachlorocyclohexanes (HCHs) and hexabromocyclododecanes (HBCDs). They all have been classified as persistent organic pollutants (POPs) under the UN Stockholm Convention. Per se such compounds are transformed slowly in the environment, transported over long distances and accumulate in biota. Several Sphingomonadacea strains isolated from HCH dump sites have evolved to express enzymes that can transform HCHs and HBCDs. We hypothesized that LinA2, a dehydrohalogenase expressed in such bacteria, may also transform CPs to chlorinated olefins (COs). Three mixtures of penta- to deca-chlorinated undecanes (C11), dodecanes (C12) and tridecanes (C13) were exposed to LinA2. High-resolution full-scan mass spectra (R∼8'000) of CPs and COs were obtained applying a soft ionization method, enhancing chloride-adduct [M+Cl]- formation. A mathematical deconvolution procedure was used to separate interfering spectra to verify that LinA2 indeed catalyzed the conversion of CPs to COs. About 20-40% of the material was transformed in 24 h, about 50-70% was converted in 200 h. A bimodal first-order kinetic model could describe transformations of reactive and persistent CPs. Under the given conditions reactive CPs (τ1/2 = 1.4-6.9 h) were converted 30 to 190-times faster than the persistent ones (τ1/2 = 150-260 h). Proportions of persistent isomers (pp) varied from 60 to 80%. Lower chlorinated homologues contained higher proportions of persistent isomers. In conclusion, SCCP mixtures contain both, material that is readily converted by LinA2, and persistent material that is not or only slowly transformed.de_CH
dc.language.isoende_CH
dc.publisherElsevierde_CH
dc.relation.ispartofChemospherede_CH
dc.rightsLicence according to publishing contractde_CH
dc.subjectBimodal first-order kineticsde_CH
dc.subjectChlorinated olefinsde_CH
dc.subjectChlorinated paraffinsde_CH
dc.subjectDeconvolution of mass spectrade_CH
dc.subjectEnzymatic transformationde_CH
dc.subjectBiotransformationde_CH
dc.subjectEnvironmental Monitoringde_CH
dc.subjectHalogenationde_CH
dc.subjectHexachlorocyclohexanede_CH
dc.subjectHydrocarbons, Brominatedde_CH
dc.subjectHydrolasede_CH
dc.subjectParaffinde_CH
dc.subject.ddc660: Technische Chemiede_CH
dc.titleBiotransformation of short-chain chlorinated paraffins (SCCPs) with LinA2 : A HCH and HBCD converting bacterial dehydrohalogenasede_CH
dc.typeBeitrag in wissenschaftlicher Zeitschriftde_CH
dcterms.typeTextde_CH
zhaw.departementLife Sciences und Facility Managementde_CH
zhaw.organisationalunitInstitut für Chemie und Biotechnologie (ICBT)de_CH
dc.identifier.doi10.1016/j.chemosphere.2019.03.169de_CH
dc.identifier.pmid30965245de_CH
zhaw.funding.euNode_CH
zhaw.originated.zhawYesde_CH
zhaw.pages.end754de_CH
zhaw.pages.start744de_CH
zhaw.publication.statuspublishedVersionde_CH
zhaw.volume226de_CH
zhaw.publication.reviewPeer review (Publikation)de_CH
zhaw.author.additionalNode_CH
zhaw.display.portraitYesde_CH
Appears in collections:Publikationen Life Sciences und Facility Management

Files in This Item:
There are no files associated with this item.
Show simple item record
Heeb, N. V., Schalles, S., Lehner, S., Schinkel, L., Schilling, I., Lienemann, P., Bogdal, C., & Kohler, H.-P. E. (2019). Biotransformation of short-chain chlorinated paraffins (SCCPs) with LinA2 : A HCH and HBCD converting bacterial dehydrohalogenase. Chemosphere, 226, 744–754. https://doi.org/10.1016/j.chemosphere.2019.03.169
Heeb, N.V. et al. (2019) ‘Biotransformation of short-chain chlorinated paraffins (SCCPs) with LinA2 : A HCH and HBCD converting bacterial dehydrohalogenase’, Chemosphere, 226, pp. 744–754. Available at: https://doi.org/10.1016/j.chemosphere.2019.03.169.
N. V. Heeb et al., “Biotransformation of short-chain chlorinated paraffins (SCCPs) with LinA2 : A HCH and HBCD converting bacterial dehydrohalogenase,” Chemosphere, vol. 226, pp. 744–754, Jul. 2019, doi: 10.1016/j.chemosphere.2019.03.169.
HEEB, Norbert V., Simone SCHALLES, Sandro LEHNER, Lena SCHINKEL, Iris SCHILLING, Peter LIENEMANN, Christian BOGDAL und Hans-Peter E. KOHLER, 2019. Biotransformation of short-chain chlorinated paraffins (SCCPs) with LinA2 : A HCH and HBCD converting bacterial dehydrohalogenase. Chemosphere. Juli 2019. Bd. 226, S. 744–754. DOI 10.1016/j.chemosphere.2019.03.169
Heeb, Norbert V., Simone Schalles, Sandro Lehner, Lena Schinkel, Iris Schilling, Peter Lienemann, Christian Bogdal, and Hans-Peter E. Kohler. 2019. “Biotransformation of Short-Chain Chlorinated Paraffins (SCCPs) with LinA2 : A HCH and HBCD Converting Bacterial Dehydrohalogenase.” Chemosphere 226 (July): 744–54. https://doi.org/10.1016/j.chemosphere.2019.03.169.
Heeb, Norbert V., et al. “Biotransformation of Short-Chain Chlorinated Paraffins (SCCPs) with LinA2 : A HCH and HBCD Converting Bacterial Dehydrohalogenase.” Chemosphere, vol. 226, July 2019, pp. 744–54, https://doi.org/10.1016/j.chemosphere.2019.03.169.


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.