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https://doi.org/10.21256/zhaw-25201| Publication type: | Master thesis |
| Title: | Cultivation of PHB-producing cyanobacteria in wastewater on laboratory and pilot-scale |
| Authors: | Mariotto, Marina |
| Advisors / Reviewers: | Refardt, Dominik Fritz, Ines Egloff, Sophia |
| DOI: | 10.21256/zhaw-25201 |
| Extent: | 29 |
| Issue Date: | 2022 |
| Publisher / Ed. Institution: | ZHAW Zürcher Hochschule für Angewandte Wissenschaften |
| Publisher / Ed. Institution: | Winterthur |
| Language: | English |
| Subjects: | Wastewater treatment; Liquid digestate; Aquaculture water; Bioplastic; Biopolymer; Nitrogen mass balance |
| Subject (DDC): | 579: Microbiology |
| Abstract: | The cultivation of PHB-producing cyanobacteria is a promising process to optain the raw material for bio-based and biodegradable plastics. To commercialise photoautotrophic PHB production, the cultivation needs to be scaled up in open cultivation systems. Further, the use of wastewater as nutrient source improves the resource efficiency of the process, making it cheaper and more sustainable. Here, the feasibility of these steps was tested. First, the suitability of different PHB-producing cyanobacteria was compared in laboratory scale cultivations. Then, the species Synechococcus leopoliensis was cultivated in an open thin-layer photobioreactor (18 m2, 200 L), using a mineral medium, water from recirculating aquaculture systems and pre-processed liquid digestate as nutrient sources. Cultivations in all three media were successful. Cultivation in mineral medium resulted in both the highest final biomass yield (6 g L-1) and productivity (0.7 g L-1 d-1). Both wastewater-based media showed lower biomass yields and productivities (2 g L-1 and 0.25–0.3 g L-1 d-1). However, due to differences in the cultivation conditions (e.g. temperature, nutrient supply), final biomass yield and productivity do not represent the performance of the cultivations adequately. Therefore, relative parameters such as nitrogen and energy conversion ratios were applied. All cultivations in the open system were continued in the laboratory, where cultures were starved for 10 days under nutrient-depleted conditions (without nitrogen, phosphorus and both). This yielded comparatively low amounts of PHB (< 1 %dw). The comparison of the results suggest that PHB yields were influenced more by the initial cultivation condition than by the specific type of nutrient depletion. Thus, while the cultivation with waste streams in an open system is feasible, environmental parameters seem to influence PHB yields considerably and must be taken into account for the optimisation of the complete process. |
| URI: | https://digitalcollection.zhaw.ch/handle/11475/25201 |
| License (according to publishing contract): | CC BY 4.0: Attribution 4.0 International |
| Departement: | Life Sciences and Facility Management |
| Appears in collections: | Masterarbeiten Umwelt und Natürliche Ressourcen |
Files in This Item:
| File | Description | Size | Format | |
|---|---|---|---|---|
| 2022_Mariotto_Mariana_MAS_ENR.pdf | 2.41 MB | Adobe PDF |  View/Open |
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Mariotto, M. (2022). Cultivation of PHB-producing cyanobacteria in wastewater on laboratory and pilot-scale [Master’s thesis, ZHAW Zürcher Hochschule für Angewandte Wissenschaften]. https://doi.org/10.21256/zhaw-25201
Mariotto, M. (2022) Cultivation of PHB-producing cyanobacteria in wastewater on laboratory and pilot-scale. Master’s thesis. ZHAW Zürcher Hochschule für Angewandte Wissenschaften. Available at: https://doi.org/10.21256/zhaw-25201.
M. Mariotto, “Cultivation of PHB-producing cyanobacteria in wastewater on laboratory and pilot-scale,” Master’s thesis, ZHAW Zürcher Hochschule für Angewandte Wissenschaften, Winterthur, 2022. doi: 10.21256/zhaw-25201.
MARIOTTO, Marina, 2022. Cultivation of PHB-producing cyanobacteria in wastewater on laboratory and pilot-scale. Master’s thesis. Winterthur: ZHAW Zürcher Hochschule für Angewandte Wissenschaften
Mariotto, Marina. 2022. “Cultivation of PHB-Producing Cyanobacteria in Wastewater on Laboratory and Pilot-Scale.” Master’s thesis, Winterthur: ZHAW Zürcher Hochschule für Angewandte Wissenschaften. https://doi.org/10.21256/zhaw-25201.
Mariotto, Marina. Cultivation of PHB-Producing Cyanobacteria in Wastewater on Laboratory and Pilot-Scale. ZHAW Zürcher Hochschule für Angewandte Wissenschaften, 2022, https://doi.org/10.21256/zhaw-25201.
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