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Please use this identifier to cite or link to this item: https://doi.org/10.21256/zhaw-21812
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dc.contributor.authorKalbermatter, David-
dc.contributor.authorShrestha, Neeta-
dc.contributor.authorGall, Flavio-
dc.contributor.authorWyss, Marianne-
dc.contributor.authorRiedl, Rainer-
dc.contributor.authorPlattet, Philippe-
dc.contributor.authorFotiadis, Dimitrios-
dc.date.accessioned2021-02-18T13:38:54Z-
dc.date.available2021-02-18T13:38:54Z-
dc.date.issued2020-02-29-
dc.identifier.issn2590-1524de_CH
dc.identifier.urihttps://digitalcollection.zhaw.ch/handle/11475/21812-
dc.description.abstractMeasles virus (MeV) and canine distemper virus (CDV), two members of the Morbillivirus genus, are still causing important global diseases of humans and animals, respectively. To enter target cells, morbilliviruses rely on an envelope-anchored machinery, which is composed of two interacting glycoproteins: a tetrameric receptor binding (H) protein and a trimeric fusion (F) protein. To execute membrane fusion, the F protein initially adopts a metastable, prefusion state that refolds into a highly stable postfusion conformation as the result of a finely coordinated activation process mediated by the H protein. Here, we employed cryo-electron microscopy (cryo-EM) and single particle reconstruction to elucidate the structure of the prefusion state of the CDV F protein ectodomain (solF) at 4.3 Å resolution. Stabilization of the prefusion solF trimer was achieved by fusing the GCNt trimerization sequence at the C-terminal protein region, and expressing and purifying the recombinant protein in the presence of a morbilliviral fusion inhibitor class compound. The three-dimensional cryo-EM map of prefusion CDV solF in complex with the inhibitor clearly shows density for the ligand at the protein binding site suggesting common mechanisms of membrane fusion activation and inhibition employed by different morbillivirus members.de_CH
dc.language.isoende_CH
dc.publisherElsevierde_CH
dc.relation.ispartofJournal of Structural Biology: Xde_CH
dc.rightshttp://creativecommons.org/licenses/by/4.0/de_CH
dc.subjectCDV, canine distemper virusde_CH
dc.subjectCanine distemper virusde_CH
dc.subjectCryo-electron microscopyde_CH
dc.subjectFP, fusion peptidede_CH
dc.subjectFusion proteinde_CH
dc.subjectMeV, measles virusde_CH
dc.subjectMorbillivirus cell entryde_CH
dc.subjectSingle particle reconstructionde_CH
dc.subjectcryo-EM, cryo-electron microscopyde_CH
dc.subject.ddc579: Mikrobiologiede_CH
dc.titleCryo-EM structure of the prefusion state of canine distemper virus fusion protein ectodomainde_CH
dc.typeBeitrag in wissenschaftlicher Zeitschriftde_CH
dcterms.typeTextde_CH
zhaw.departementLife Sciences und Facility Managementde_CH
zhaw.organisationalunitInstitut für Chemie und Biotechnologie (ICBT)de_CH
dc.identifier.doi10.1016/j.yjsbx.2020.100021de_CH
dc.identifier.doi10.21256/zhaw-21812-
dc.identifier.pmid32647825de_CH
zhaw.funding.euNode_CH
zhaw.originated.zhawYesde_CH
zhaw.pages.start100021de_CH
zhaw.publication.statuspublishedVersionde_CH
zhaw.volume4de_CH
zhaw.publication.reviewPeer review (Publikation)de_CH
zhaw.funding.snf183481de_CH
zhaw.webfeedCC Drug Discoveryde_CH
zhaw.author.additionalNode_CH
zhaw.display.portraitYesde_CH
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Kalbermatter, D., Shrestha, N., Gall, F., Wyss, M., Riedl, R., Plattet, P., & Fotiadis, D. (2020). Cryo-EM structure of the prefusion state of canine distemper virus fusion protein ectodomain. Journal of Structural Biology: X, 4, 100021. https://doi.org/10.1016/j.yjsbx.2020.100021
Kalbermatter, D. et al. (2020) ‘Cryo-EM structure of the prefusion state of canine distemper virus fusion protein ectodomain’, Journal of Structural Biology: X, 4, p. 100021. Available at: https://doi.org/10.1016/j.yjsbx.2020.100021.
D. Kalbermatter et al., “Cryo-EM structure of the prefusion state of canine distemper virus fusion protein ectodomain,” Journal of Structural Biology: X, vol. 4, p. 100021, Feb. 2020, doi: 10.1016/j.yjsbx.2020.100021.
KALBERMATTER, David, Neeta SHRESTHA, Flavio GALL, Marianne WYSS, Rainer RIEDL, Philippe PLATTET und Dimitrios FOTIADIS, 2020. Cryo-EM structure of the prefusion state of canine distemper virus fusion protein ectodomain. Journal of Structural Biology: X. 29 Februar 2020. Bd. 4, S. 100021. DOI 10.1016/j.yjsbx.2020.100021
Kalbermatter, David, Neeta Shrestha, Flavio Gall, Marianne Wyss, Rainer Riedl, Philippe Plattet, and Dimitrios Fotiadis. 2020. “Cryo-EM Structure of the Prefusion State of Canine Distemper Virus Fusion Protein Ectodomain.” Journal of Structural Biology: X 4 (February): 100021. https://doi.org/10.1016/j.yjsbx.2020.100021.
Kalbermatter, David, et al. “Cryo-EM Structure of the Prefusion State of Canine Distemper Virus Fusion Protein Ectodomain.” Journal of Structural Biology: X, vol. 4, Feb. 2020, p. 100021, https://doi.org/10.1016/j.yjsbx.2020.100021.


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