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dc.contributor.authorBentebibel, Salima-
dc.contributor.authorMoyano, Elisabet-
dc.contributor.authorPalazón, Javier-
dc.contributor.authorCusidó, Rosa M.-
dc.contributor.authorBonfill, Mercedes-
dc.contributor.authorEibl-Schindler, Regine-
dc.contributor.authorPiñol, M. Teresa-
dc.date.accessioned2018-01-17T12:38:11Z-
dc.date.available2018-01-17T12:38:11Z-
dc.date.issued2005-
dc.identifier.issn0006-3592de_CH
dc.identifier.issn1097-0290de_CH
dc.identifier.urihttps://digitalcollection.zhaw.ch/handle/11475/2098-
dc.description.abstractPaclitaxel and baccatin III-producing cells of Taxus baccata were immobilized within Ca2+-alginate beads. Under established optimum conditions for the biosynthesis of both taxanes, the yields of paclitaxel and baccatin III in shake-flask cultures of free cells increased by factors of up to 3 and 2, respectively, in the corresponding cultures of immobilized cells. Although the scale-up from shake-flask to bioreactor culture usually results in reduced productivities when both free and immobilized cells were grown in the same optimum conditions in three different bioreactor types (Stirred, Airlift, and Wave) running for 24 days in a batch mode and with the system optimized in each case, there was a considerable increase in the yields of paclitaxel and baccatin III. Among the reactors, the Stirred bioreactor was the most efficient in promoting immobilized cell production of paclitaxel, giving a content of 43.43 mg.L−1 at 16 days of culture, equivalent to a rate of 2.71 mg.L−1.day−1. To our knowledge, the paclitaxel productivity obtained in this study is one of the highest reported so far by academic laboratories for Taxus species cultures in bioreactors.de_CH
dc.language.isoende_CH
dc.publisherWileyde_CH
dc.relation.ispartofBiotechnology & Bioengineeringde_CH
dc.rightsLicence according to publishing contractde_CH
dc.subject.ddc660.6: Biotechnologiede_CH
dc.titleEffects of immobilization by entrapment in alginate and scale-up on paclitaxel and baccatin III production in cell suspension cultures of Taxus baccatade_CH
dc.typeBeitrag in wissenschaftlicher Zeitschriftde_CH
dcterms.typeTextde_CH
zhaw.departementLife Sciences und Facility Managementde_CH
dc.identifier.doi10.1002/bit.20321de_CH
zhaw.funding.euNode_CH
zhaw.issue6de_CH
zhaw.originated.zhawYesde_CH
zhaw.pages.end655de_CH
zhaw.pages.start647de_CH
zhaw.publication.statuspublishedVersionde_CH
zhaw.volume89de_CH
zhaw.publication.reviewPeer review (Publikation)de_CH
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Bentebibel, S., Moyano, E., Palazón, J., Cusidó, R. M., Bonfill, M., Eibl-Schindler, R., & Piñol, M. T. (2005). Effects of immobilization by entrapment in alginate and scale-up on paclitaxel and baccatin III production in cell suspension cultures of Taxus baccata. Biotechnology & Bioengineering, 89(6), 647–655. https://doi.org/10.1002/bit.20321
Bentebibel, S. et al. (2005) ‘Effects of immobilization by entrapment in alginate and scale-up on paclitaxel and baccatin III production in cell suspension cultures of Taxus baccata’, Biotechnology & Bioengineering, 89(6), pp. 647–655. Available at: https://doi.org/10.1002/bit.20321.
S. Bentebibel et al., “Effects of immobilization by entrapment in alginate and scale-up on paclitaxel and baccatin III production in cell suspension cultures of Taxus baccata,” Biotechnology & Bioengineering, vol. 89, no. 6, pp. 647–655, 2005, doi: 10.1002/bit.20321.
BENTEBIBEL, Salima, Elisabet MOYANO, Javier PALAZÓN, Rosa M. CUSIDÓ, Mercedes BONFILL, Regine EIBL-SCHINDLER und M. Teresa PIÑOL, 2005. Effects of immobilization by entrapment in alginate and scale-up on paclitaxel and baccatin III production in cell suspension cultures of Taxus baccata. Biotechnology & Bioengineering. 2005. Bd. 89, Nr. 6, S. 647–655. DOI 10.1002/bit.20321
Bentebibel, Salima, Elisabet Moyano, Javier Palazón, Rosa M. Cusidó, Mercedes Bonfill, Regine Eibl-Schindler, and M. Teresa Piñol. 2005. “Effects of Immobilization by Entrapment in Alginate and Scale-up on Paclitaxel and Baccatin III Production in Cell Suspension Cultures of Taxus Baccata.” Biotechnology & Bioengineering 89 (6): 647–55. https://doi.org/10.1002/bit.20321.
Bentebibel, Salima, et al. “Effects of Immobilization by Entrapment in Alginate and Scale-up on Paclitaxel and Baccatin III Production in Cell Suspension Cultures of Taxus Baccata.” Biotechnology & Bioengineering, vol. 89, no. 6, 2005, pp. 647–55, https://doi.org/10.1002/bit.20321.


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