Host–pathogen interactions between Xanthomonas fragariae and its host Fragaria × ananassa investigated with a dual RNA-Seq analysis

Gétaz, Michael; Puławska, Joanna; Smits, Theo H.M.; Pothier, Joël F.

doi:10.3390/microorganisms8081253

Please use this identifier to cite or link to this item: https://doi.org/10.21256/zhaw-20382

Full metadata record

DC Field	Value	Language
dc.contributor.author	Gétaz, Michael	-
dc.contributor.author	Puławska, Joanna	-
dc.contributor.author	Smits, Theo H.M.	-
dc.contributor.author	Pothier, Joël F.	-
dc.date.accessioned	2020-08-24T08:26:26Z	-
dc.date.available	2020-08-24T08:26:26Z	-
dc.date.issued	2020-08-18	-
dc.identifier.issn	2076-2607	de_CH
dc.identifier.uri	https://digitalcollection.zhaw.ch/handle/11475/20382	-
dc.description.abstract	Strawberry is economically important and widely grown, but susceptible to a large variety of phytopathogenic organisms. Among them, Xanthomonas fragariae is a quarantine bacterial pathogen threatening strawberry productions by causing angular leaf spots. Using whole transcriptome sequencing, the gene expression of both plant and bacteria in planta was analyzed at two time points, 12 and 29 days post inoculation, in order to compare the pathogen and host response between the stages of early visible and of well-developed symptoms. Among 28,588 known genes in strawberry and 4046 known genes in X. fragariae expressed at both time points, a total of 361 plant and 144 bacterial genes were significantly differentially expressed, respectively. The identified higher expressed genes in the plants were pathogen-associated molecular pattern receptors and pathogenesis-related thaumatin encoding genes, whereas the more expressed early genes were related to chloroplast metabolism as well as photosynthesis related coding genes. Most X. fragariae genes involved in host interaction, recognition, and pathogenesis were lower expressed at late-phase infection. This study gives a first insight into the interaction of X. fragariae with its host. The strawberry plant changed gene expression in order to consistently adapt its metabolism with the progression of infection.	de_CH
dc.language.iso	en	de_CH
dc.publisher	MDPI	de_CH
dc.relation.ispartof	Microorganisms	de_CH
dc.rights	http://creativecommons.org/licenses/by/4.0/	de_CH
dc.subject	Strawberry	de_CH
dc.subject	Plant inoculation	de_CH
dc.subject	Transcriptome	de_CH
dc.subject	RNA-sequencing	de_CH
dc.subject	Virulence factor	de_CH
dc.subject.ddc	572: Biochemie	de_CH
dc.title	Host–pathogen interactions between Xanthomonas fragariae and its host Fragaria × ananassa investigated with a dual RNA-Seq analysis	de_CH
dc.type	Beitrag in wissenschaftlicher Zeitschrift	de_CH
dcterms.type	Text	de_CH
zhaw.departement	Life Sciences und Facility Management	de_CH
zhaw.organisationalunit	Institut für Umwelt und Natürliche Ressourcen (IUNR)	de_CH
dc.identifier.doi	10.3390/microorganisms8081253	de_CH
dc.identifier.doi	10.21256/zhaw-20382	-
zhaw.funding.eu	info:eu-repo/grantAgreement/EC/FP7/613678//Strategies to develop effective, innovative and practical approaches to protect major European fruit crops from pests and pathogens/DROPSA	de_CH
zhaw.issue	8	de_CH
zhaw.originated.zhaw	Yes	de_CH
zhaw.pages.start	1253	de_CH
zhaw.publication.status	publishedVersion	de_CH
zhaw.volume	8	de_CH
zhaw.publication.review	Peer review (Publikation)	de_CH
zhaw.webfeed	Umweltgenomik	de_CH
zhaw.funding.zhaw	DROPSA – Strategies to develop effective, innovative and practical approaches to protect major European fruit crops from pests and pathogens	de_CH
zhaw.author.additional	No	de_CH
zhaw.display.portrait	Yes	de_CH
zhaw.relation.references	https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE150636	de_CH
Appears in collections:	Publikationen Life Sciences und Facility Management

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Gétaz, M., Puławska, J., Smits, T. H. M., & Pothier, J. F. (2020). Host–pathogen interactions between Xanthomonas fragariae and its host Fragaria × ananassa investigated with a dual RNA-Seq analysis. Microorganisms, 8(8), 1253. https://doi.org/10.3390/microorganisms8081253

Gétaz, M. et al. (2020) ‘Host–pathogen interactions between Xanthomonas fragariae and its host Fragaria × ananassa investigated with a dual RNA-Seq analysis’, Microorganisms, 8(8), p. 1253. Available at: https://doi.org/10.3390/microorganisms8081253.

M. Gétaz, J. Puławska, T. H. M. Smits, and J. F. Pothier, “Host–pathogen interactions between Xanthomonas fragariae and its host Fragaria × ananassa investigated with a dual RNA-Seq analysis,” Microorganisms, vol. 8, no. 8, p. 1253, Aug. 2020, doi: 10.3390/microorganisms8081253.

GÉTAZ, Michael, Joanna PUŁAWSKA, Theo H.M. SMITS und Joël F. POTHIER, 2020. Host–pathogen interactions between Xanthomonas fragariae and its host Fragaria × ananassa investigated with a dual RNA-Seq analysis. Microorganisms. 18 August 2020. Bd. 8, Nr. 8, S. 1253. DOI 10.3390/microorganisms8081253

Gétaz, Michael, Joanna Puławska, Theo H.M. Smits, and Joël F. Pothier. 2020. “Host–Pathogen Interactions between Xanthomonas Fragariae and Its Host Fragaria × Ananassa Investigated with a Dual RNA-Seq Analysis.” Microorganisms 8 (8): 1253. https://doi.org/10.3390/microorganisms8081253.

Gétaz, Michael, et al. “Host–Pathogen Interactions between Xanthomonas Fragariae and Its Host Fragaria × Ananassa Investigated with a Dual RNA-Seq Analysis.” Microorganisms, vol. 8, no. 8, Aug. 2020, p. 1253, https://doi.org/10.3390/microorganisms8081253.