Publication type: | Article in scientific journal |
Type of review: | Peer review (publication) |
Title: | Recent advances in effect-directed enzyme assays based on thin-layer chromatography |
Authors: | Wolfram, Evelyn Bräm, Sarah |
DOI: | 10.1002/pca.2669 |
Published in: | Phytochemical Analysis |
Volume(Issue): | 28 |
Issue: | 2 |
Page(s): | 74 |
Pages to: | 86 |
Issue Date: | 1-Feb-2017 |
Publisher / Ed. Institution: | Wiley |
ISSN: | 1099-1565 0958-0344 |
Language: | English |
Subjects: | Drug Discovery; Bioautography; Enzyme assay; HPTLC |
Subject (DDC): | 572: Biochemistry 580: Plants (Botany) 660.6: Biotechnology |
Abstract: | Thin-layer chromatography (TLC) together with its more modern form HPTLC is a rapid and cost effective analytical tool with a long tradition in quality control of medicinal plants, extracts and natural products. Separated compounds are fixed on the solid silica phase to form a compound library. Through direct coupling of visualizable enzyme reactions on the TLC plate, this compound library can also be used for activity screening. Such TLC-based bioautographic enzyme and enzyme inhibition assays complement first stage development activity screening assays. They provide not only phytochemical results by chromatographic separation, but also additional information about the activity of constituents or fractions in multi-compound mixtures, and thus can reveal and distinguish artefacts generated by certain compound classes. This review summarizes recently introduced TLC bioautographic enzyme assays as well as advances in already existing procedures. Bioautographic enzyme and enzyme inhibitory assays offer a rapid, high-throughput method for screening of secondary metabolite profiles for potential enzyme and enzyme inhibitory activities. |
URI: | https://digitalcollection.zhaw.ch/handle/11475/1959 |
Fulltext version: | Published version |
License (according to publishing contract): | Licence according to publishing contract |
Departement: | Life Sciences and Facility Management |
Organisational Unit: | Institute of Chemistry and Biotechnology (ICBT) |
Appears in collections: | Publikationen Life Sciences und Facility Management |
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Wolfram, E., & Bräm, S. (2017). Recent advances in effect-directed enzyme assays based on thin-layer chromatography. Phytochemical Analysis, 28(2), 74–86. https://doi.org/10.1002/pca.2669
Wolfram, E. and Bräm, S. (2017) ‘Recent advances in effect-directed enzyme assays based on thin-layer chromatography’, Phytochemical Analysis, 28(2), pp. 74–86. Available at: https://doi.org/10.1002/pca.2669.
E. Wolfram and S. Bräm, “Recent advances in effect-directed enzyme assays based on thin-layer chromatography,” Phytochemical Analysis, vol. 28, no. 2, pp. 74–86, Feb. 2017, doi: 10.1002/pca.2669.
WOLFRAM, Evelyn und Sarah BRÄM, 2017. Recent advances in effect-directed enzyme assays based on thin-layer chromatography. Phytochemical Analysis. 1 Februar 2017. Bd. 28, Nr. 2, S. 74–86. DOI 10.1002/pca.2669
Wolfram, Evelyn, and Sarah Bräm. 2017. “Recent Advances in Effect-Directed Enzyme Assays Based on Thin-Layer Chromatography.” Phytochemical Analysis 28 (2): 74–86. https://doi.org/10.1002/pca.2669.
Wolfram, Evelyn, and Sarah Bräm. “Recent Advances in Effect-Directed Enzyme Assays Based on Thin-Layer Chromatography.” Phytochemical Analysis, vol. 28, no. 2, Feb. 2017, pp. 74–86, https://doi.org/10.1002/pca.2669.
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