|Title:||Methods for detecting fungi in turf grass with a lamp assay having novel primer sets|
|Inventor :||Palmisano, Marilena|
|Patent submitter:||SYNGENTA PARTICIPATIONS AG, Basel|
|Language :||English |
|Subjects :||LAMP, grass, detection, pathogen|
|Subject (DDC) :||570: Biology|
|Abstract:||The present invention provides a method for detecting fungal DNA in a turf grass sample with a loop- mediated isothermal amplification (LAMP) assay which contains primers for fungal DNA of at least one turf pathogenic fungi selected from Sclerotinia homoeocarpa, Rhizoctonia solani spp., Pythium aphanidermatum, Gaeumannomyces graminis spp., Microdochium nivale spp., Magnaporthe poae, Colletotrichum graminicola, Colletotrichum cereale and Pythium ultimum var. ultimum, comprising: subjecting the turf sample to a LAMP reaction wherein the LAMP reaction uses a primer set of four or more nucleic acid sequences with each primer in the set having from 15 to 50 nucleic acids The primers useful in the present method are selected from specifically selected internal transcribed spacer regions or genes of the target fungi to provide improved assay results.|
|Departement:||Life Sciences and Facility Management|
|Organisational Unit:||Institute of Natural Resource Sciences (IUNR)|
|Published as part of the ZHAW project :||Entwicklung eines Turf-Arrays zur Parallelidentifikation von global relevanten Rasenkrankheiten|
|Appears in Collections:||Patente Life Sciences und Facility Management|
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