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  2. School of Engineering
  3. Publikationen
  4. Publikationen School of Engineering
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dc.contributor.authorBally, Marta-
dc.contributor.authorSyed, Shahida-
dc.contributor.authorBinkert, Andreas-
dc.contributor.authorKauffmann, Ekkehard-
dc.contributor.authorEhrat, Markus-
dc.contributor.authorVörös, Janos-
dc.date.accessioned2017-12-07T10:05:46Z-
dc.date.available2017-12-07T10:05:46Z-
dc.date.issued2011-
dc.identifier.issn0003-2697de_CH
dc.identifier.issn1096-0309de_CH
dc.identifier.urihttps://digitalcollection.zhaw.ch/handle/11475/1734-
dc.description.abstractDevelopments in microarray technology promise to lead to great advancements in the biomedical and biological field. However, implementation of these analytical tools often relies on signal amplification strategies that are essential to reach the sensitivity levels required for a variety of biological applications. This is true especially for reverse phase arrays where a complex biological sample is directly immobilized on the chip. We present a simple and generic method for signal amplification based on the use of antibody-tagged fluorescent vesicles as labels for signal generation. To assess the gain in assay sensitivity, we performed a model assay for the detection of rabbit immunoglobulin G (IgG) and compared the limit of detection (LOD) of the vesicle assay with the LOD of a conventional assay performed with fluorescent reporter molecules. We evaluated the improvements for two fluorescence-based transduction setups: a high-sensitivity microarray reader (ZeptoREADER) and a conventional confocal scanner. In all cases, our strategy led to an increase in sensitivity. However, gain in sensitivity widely depended on the type of illumination; whereas an approximately 2-fold increase in sensitivity was observed for readout based on evanescent field illumination, the contribution was as high as more than 200-fold for confocal scanning.de_CH
dc.language.isoende_CH
dc.publisherElsevierde_CH
dc.relation.ispartofAnalytical Biochemistryde_CH
dc.rightsLicence according to publishing contractde_CH
dc.subjectAnimalde_CH
dc.subjectAntibodiesde_CH
dc.subjectFluorescent Dyede_CH
dc.subjectImmunoassayde_CH
dc.subjectImmunoglobulin Gde_CH
dc.subjectLipid Bilayerde_CH
dc.subjectMicroscopy, Confocalde_CH
dc.subjectProtein Array Analysisde_CH
dc.subjectRabbitde_CH
dc.subjectRhodaminesde_CH
dc.subject.ddc570: Biologiede_CH
dc.subject.ddc615: Pharmakologie und Therapeutikde_CH
dc.titleFluorescent vesicles for signal amplification in reverse phase protein microarray assaysde_CH
dc.typeBeitrag in wissenschaftlicher Zeitschriftde_CH
dcterms.typeTextde_CH
zhaw.departementSchool of Engineeringde_CH
dc.identifier.doi10.1016/j.ab.2011.05.030de_CH
dc.identifier.pmid21669176de_CH
zhaw.funding.euNode_CH
zhaw.issue2de_CH
zhaw.originated.zhawNode_CH
zhaw.pages.end151de_CH
zhaw.pages.start145de_CH
zhaw.publication.statuspublishedVersionde_CH
zhaw.volume416de_CH
zhaw.publication.reviewPeer review (Publikation)de_CH
zhaw.webfeedDiagnostik und Beratungde_CH
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Bally, M., Syed, S., Binkert, A., Kauffmann, E., Ehrat, M., & Vörös, J. (2011). Fluorescent vesicles for signal amplification in reverse phase protein microarray assays. Analytical Biochemistry, 416(2), 145–151. https://doi.org/10.1016/j.ab.2011.05.030
Bally, M. et al. (2011) ‘Fluorescent vesicles for signal amplification in reverse phase protein microarray assays’, Analytical Biochemistry, 416(2), pp. 145–151. Available at: https://doi.org/10.1016/j.ab.2011.05.030.
M. Bally, S. Syed, A. Binkert, E. Kauffmann, M. Ehrat, and J. Vörös, “Fluorescent vesicles for signal amplification in reverse phase protein microarray assays,” Analytical Biochemistry, vol. 416, no. 2, pp. 145–151, 2011, doi: 10.1016/j.ab.2011.05.030.
BALLY, Marta, Shahida SYED, Andreas BINKERT, Ekkehard KAUFFMANN, Markus EHRAT und Janos VÖRÖS, 2011. Fluorescent vesicles for signal amplification in reverse phase protein microarray assays. Analytical Biochemistry. 2011. Bd. 416, Nr. 2, S. 145–151. DOI 10.1016/j.ab.2011.05.030
Bally, Marta, Shahida Syed, Andreas Binkert, Ekkehard Kauffmann, Markus Ehrat, and Janos Vörös. 2011. “Fluorescent Vesicles for Signal Amplification in Reverse Phase Protein Microarray Assays.” Analytical Biochemistry 416 (2): 145–51. https://doi.org/10.1016/j.ab.2011.05.030.
Bally, Marta, et al. “Fluorescent Vesicles for Signal Amplification in Reverse Phase Protein Microarray Assays.” Analytical Biochemistry, vol. 416, no. 2, 2011, pp. 145–51, https://doi.org/10.1016/j.ab.2011.05.030.


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