| Publikationstyp: | Beitrag in wissenschaftlicher Zeitschrift |
| Art der Begutachtung: | Peer review (Publikation) |
| Titel: | A bioreactor test system to mimic the biological and mechanical environment of oral soft tissues and to evaluate substitutes for connective tissue grafts |
| Autor/-in: | Mathes, Stephanie H. Wohlwend, Lorenz Uebersax, Lorenz von Mentlen, Roger Thoma, Daniel S. Jung, Ronald E. Görlach, Christoph Graf-Hausner, Ursula |
| DOI: | 10.1002/bit.22893 |
| Erschienen in: | Biotechnology & Bioengineering |
| Band(Heft): | 107 |
| Heft: | 6 |
| Seite(n): | 1029 |
| Seiten bis: | 1039 |
| Erscheinungsdatum: | 2010 |
| Verlag / Hrsg. Institution: | Wiley |
| ISSN: | 0006-3592 1097-0290 |
| Sprache: | Englisch |
| Schlagwörter: | Connective tissue; Human; Mouth mucosa; Organ culture technique; Mechanical stress; Physiological stress; Transplant; Bioreactor |
| Fachgebiet (DDC): | 571: Physiologie und verwandte Themen 660.6: Biotechnologie |
| Zusammenfassung: | Gingival cells of the oral connective tissue are exposed to complex mechanical forces during mastication, speech, tooth movement and orthodontic treatments. Especially during wound healing following surgical procedures, internal and external forces may occur, creating pressure upon the newly formed tissue. This clinical situation has to be considered when developing biomaterials to augment soft tissue in the oral cavity. In order to pre-evaluate a collagen sponge intended to serve as a substitute for autogenous connective tissue grafts (CTGs), a dynamic bioreactor system was developed. Pressure and shear forces can be applied in this bioreactor in addition to a constant medium perfusion to cell-material constructs. Three-dimensional volume changes and stiffness of the matrices were analyzed. In addition, cell responses such as cell vitality and extracellular matrix (ECM) production were investigated. The number of metabolic active cells constantly increased under fully dynamic culture conditions. The sponges remained elastic even after mechanical forces were applied for 14 days. Analysis of collagen type I and fibronectin revealed a statistically significant accumulation of these ECM molecules (P < 0.05-0.001) when compared to static cultures. An increased expression of tenascin-c, indicating tissue remodeling processes, was observed under dynamic conditions only. The results indicate that the tested in vitro cell culture system was able to mimic both the biological and mechanical environments of the clinical situation in a healing wound. |
| URI: | https://digitalcollection.zhaw.ch/handle/11475/16378 |
| Volltext Version: | Publizierte Version |
| Lizenz (gemäss Verlagsvertrag): | Lizenz gemäss Verlagsvertrag |
| Departement: | Life Sciences und Facility Management School of Engineering |
| Organisationseinheit: | Institut für Chemie und Biotechnologie (ICBT) Institut für Mechanische Systeme (IMES) |
| Enthalten in den Sammlungen: | Publikationen School of Engineering |
Dateien zu dieser Ressource:
Es gibt keine Dateien zu dieser Ressource.
Zur Langanzeige
Mathes, S. H., Wohlwend, L., Uebersax, L., von Mentlen, R., Thoma, D. S., Jung, R. E., Görlach, C., & Graf-Hausner, U. (2010). A bioreactor test system to mimic the biological and mechanical environment of oral soft tissues and to evaluate substitutes for connective tissue grafts. Biotechnology & Bioengineering, 107(6), 1029–1039. https://doi.org/10.1002/bit.22893
Mathes, S.H. et al. (2010) ‘A bioreactor test system to mimic the biological and mechanical environment of oral soft tissues and to evaluate substitutes for connective tissue grafts’, Biotechnology & Bioengineering, 107(6), pp. 1029–1039. Available at: https://doi.org/10.1002/bit.22893.
S. H. Mathes et al., “A bioreactor test system to mimic the biological and mechanical environment of oral soft tissues and to evaluate substitutes for connective tissue grafts,” Biotechnology & Bioengineering, vol. 107, no. 6, pp. 1029–1039, 2010, doi: 10.1002/bit.22893.
MATHES, Stephanie H., Lorenz WOHLWEND, Lorenz UEBERSAX, Roger VON MENTLEN, Daniel S. THOMA, Ronald E. JUNG, Christoph GÖRLACH und Ursula GRAF-HAUSNER, 2010. A bioreactor test system to mimic the biological and mechanical environment of oral soft tissues and to evaluate substitutes for connective tissue grafts. Biotechnology & Bioengineering. 2010. Bd. 107, Nr. 6, S. 1029–1039. DOI 10.1002/bit.22893
Mathes, Stephanie H., Lorenz Wohlwend, Lorenz Uebersax, Roger von Mentlen, Daniel S. Thoma, Ronald E. Jung, Christoph Görlach, and Ursula Graf-Hausner. 2010. “A Bioreactor Test System to Mimic the Biological and Mechanical Environment of Oral Soft Tissues and to Evaluate Substitutes for Connective Tissue Grafts.” Biotechnology & Bioengineering 107 (6): 1029–39. https://doi.org/10.1002/bit.22893.
Mathes, Stephanie H., et al. “A Bioreactor Test System to Mimic the Biological and Mechanical Environment of Oral Soft Tissues and to Evaluate Substitutes for Connective Tissue Grafts.” Biotechnology & Bioengineering, vol. 107, no. 6, 2010, pp. 1029–39, https://doi.org/10.1002/bit.22893.
Alle Ressourcen in diesem Repository sind urheberrechtlich geschützt, soweit nicht anderweitig angezeigt.