Publikationstyp: Beitrag in wissenschaftlicher Zeitschrift
Art der Begutachtung: Peer review (Publikation)
Titel: A rationally designed oligopeptide shows significant conformational changes upon binding to sulphate ions
Autor/-in: Demuth, Caspar
Zerbe, Oliver
Rognan, Didier
Söll, Richard
Beck-Sickinger, Annette
Folkers, Gerd
Spichiger, Ursula E.
DOI: 10.1016/S0956-5663(01)00221-4
Erschienen in: Biosensors and Bioelectronics
Band(Heft): 16
Heft: 9-12
Seite(n): 783
Seiten bis: 789
Erscheinungsdatum: 2001
Verlag / Hrsg. Institution: Elsevier
ISSN: 0956-5663
Sprache: Englisch
Fachgebiet (DDC): 540: Chemie
Zusammenfassung: Oligopeptides that interact with oxoanions were developed by rational design methods. The substrate-binding site of the enzyme purine nucleoside phosphorylase served as a model for the design of the ionophores. The amino acids involved in the complexation of oxoanions were linked through flexible spacer residues. These spacers were chosen such that the relative orientation of the interacting amino acids was conserved. Several peptide sequences were preselected based on intermolecular H-bond frequencies. These frequencies were calculated from molecular dynamics trajectories of the corresponding peptide-anion complexes and used to score the binding properties of the peptides. The most promising peptides were prepared using solid phase peptide synthesis. Anion binding of the peptide ionophores was screened using circular dichroism (CD) and confirmed by NMR spectroscopy. CD measurements performed in methanol revealed a significant conformational change of a linear undecapeptide upon binding to sulphate ions. Two-dimensional-NMR experiments confirmed that a conformation with high helical content is formed in the presence of sulphate ions. These conformational changes induced by the anion stimulate the development of new transduction mechanisms in chemical sensors.
Volltext Version: Publizierte Version
Lizenz (gemäss Verlagsvertrag): Lizenz gemäss Verlagsvertrag
Departement: Life Sciences und Facility Management
Organisationseinheit: Institut für Chemie und Biotechnologie (ICBT)
Enthalten in den Sammlungen:Publikationen Life Sciences und Facility Management

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