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dc.contributor.authorPrummer, Michael-
dc.contributor.authorSick, Beate-
dc.contributor.authorHecht, Bert-
dc.contributor.authorWild, Urs P.-
dc.date.accessioned2018-12-20T09:07:09Z-
dc.date.available2018-12-20T09:07:09Z-
dc.date.issued2003-
dc.identifier.issn0021-9606de_CH
dc.identifier.issn1520-9032de_CH
dc.identifier.issn1089-7690de_CH
dc.identifier.urihttps://digitalcollection.zhaw.ch/handle/11475/14034-
dc.description.abstractWe apply the concept of tomography to polarization-sensitive optical microscopy of single fluorophores to determine the three-dimensional orientation of molecular absorption dipoles with isotropic sensitivity. Wide-field microscopy provides the opportunity to monitor simultaneously three-dimensional rotation and two-dimensional translation of many molecules in parallel. For orientation determination the molecules are illuminated from different directions of incidence with linearly polarized light. In each exposure the excitation along a particular projection of the absorption dipole on the electric field leads to a distinct fluorescence intensity. Five exposures are sufficient to determine the full orientation of the fluorophores. To demonstrate the potential of the method we determine the orientation and position of individual immobilized lipid membrane markers. The shot-noise-limited isotropic angular resolution is 2°. For time-resolved studies the bandwidth can be expanded up to 200 Hz.de_CH
dc.language.isoende_CH
dc.publisherAmerican Institute of Physicsde_CH
dc.relation.ispartofThe Journal of Chemical Physicsde_CH
dc.rightsLicence according to publishing contractde_CH
dc.subjectOpticsde_CH
dc.subjectMicroscopyde_CH
dc.subjectSingle moleculede_CH
dc.subjectMulti parameterde_CH
dc.subject.ddc540: Chemiede_CH
dc.titleThree-dimensional optical polarization tomography of single moleculesde_CH
dc.typeBeitrag in wissenschaftlicher Zeitschriftde_CH
dcterms.typeTextde_CH
zhaw.departementSchool of Engineeringde_CH
zhaw.organisationalunitInstitut für Datenanalyse und Prozessdesign (IDP)de_CH
dc.identifier.doi10.1063/1.1569848de_CH
zhaw.funding.euNode_CH
zhaw.issue21de_CH
zhaw.originated.zhawNode_CH
zhaw.pages.start9824de_CH
zhaw.publication.statuspublishedVersionde_CH
zhaw.volume118de_CH
zhaw.publication.reviewPeer review (Publikation)de_CH
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Prummer, M., Sick, B., Hecht, B., & Wild, U. P. (2003). Three-dimensional optical polarization tomography of single molecules. The Journal of Chemical Physics, 118(21), 9824. https://doi.org/10.1063/1.1569848
Prummer, M. et al. (2003) ‘Three-dimensional optical polarization tomography of single molecules’, The Journal of Chemical Physics, 118(21), p. 9824. Available at: https://doi.org/10.1063/1.1569848.
M. Prummer, B. Sick, B. Hecht, and U. P. Wild, “Three-dimensional optical polarization tomography of single molecules,” The Journal of Chemical Physics, vol. 118, no. 21, p. 9824, 2003, doi: 10.1063/1.1569848.
PRUMMER, Michael, Beate SICK, Bert HECHT und Urs P. WILD, 2003. Three-dimensional optical polarization tomography of single molecules. The Journal of Chemical Physics. 2003. Bd. 118, Nr. 21, S. 9824. DOI 10.1063/1.1569848
Prummer, Michael, Beate Sick, Bert Hecht, and Urs P. Wild. 2003. “Three-Dimensional Optical Polarization Tomography of Single Molecules.” The Journal of Chemical Physics 118 (21): 9824. https://doi.org/10.1063/1.1569848.
Prummer, Michael, et al. “Three-Dimensional Optical Polarization Tomography of Single Molecules.” The Journal of Chemical Physics, vol. 118, no. 21, 2003, p. 9824, https://doi.org/10.1063/1.1569848.


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