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dc.contributor.authorAlbert, Ina-
dc.contributor.authorHefti, Martin-
dc.contributor.authorLuginbühl, Vera-
dc.date.accessioned2018-11-16T11:06:53Z-
dc.date.available2018-11-16T11:06:53Z-
dc.date.issued2014-
dc.identifier.issn0161-6412de_CH
dc.identifier.issn1743-1328de_CH
dc.identifier.urihttps://digitalcollection.zhaw.ch/handle/11475/12918-
dc.description.abstractObjectives: The partial pressure of oxygen (pO2) in brain tumors ranges from 5 to 15%. Nevertheless, the majority of in vitro experiments with glioblastoma multiforme (GBM) cell lines are carried out under an atmospheric pO2 of 19 to 21%. Recently, 5-aminolevulinic acid (5-ALA), a precursor of protoporphyrin IX (PpIX), has been introduced to neurosurgery to allow for photodynamic diagnosis and photodynamic therapy (PDT) in high-grade gliomas. Here, we investigate whether low pO2 affects GBM cell physiology, PpIX accumulation, or PDT efficacy. Methods: GBM cell lines (U-87 MG and U-251 MG) were cultured under atmospheric (pO2  =  19%) and physiological (pO2  =  9%) oxygen concentrations. PpIX accumulation and localization were investigated, and cell survival and cell death were observed following in vitro PDT. Results: A physiological pO2 of 9% stimulated GBM cell migration, increased hypoxia-inducible factor (HIF)-1 alpha levels, and elevated resistance to camptothecin in U-87 MG cells compared to cultivation at a pO2 of 19%. This oxygen reduction did not alter 5-ALA-induced intracellular PpIX accumulation. However, physiological pO2 changed the responsiveness of U-87 MG but not of U-251 MG cells to in vitro PDT. Around 20% more irradiation light was required to kill U-87 MG cells at physiological pO2, resulting in reduced lactate dehydrogenase (LDH) release (one-to two-fold) and inhibition of caspase 3 activation. Discussion: Reduction of oxygen concentration from atmospheric to a more physiological level can influence the malignant behavior and survival of GBM cell lines after in vitro PDT. Therefore, precise oxygen concentration control should be considered when designing and performing experiments with GBM cells.de_CH
dc.language.isoende_CH
dc.publisherTaylor & Francisde_CH
dc.relation.ispartofNeurological Researchde_CH
dc.rightsLicence according to publishing contractde_CH
dc.subjectGlioblastomade_CH
dc.subjectPhotodynamic therapyde_CH
dc.subject5-Aminolevulinic acidde_CH
dc.subjectHypoxiade_CH
dc.subject.ddc615: Pharmakologie und Therapeutikde_CH
dc.subject.ddc616: Innere Medizin und Krankheitende_CH
dc.titlePhysiological oxygen concentration alters glioma cell malignancy and responsiveness to photodynamic therapy in vitrode_CH
dc.typeBeitrag in wissenschaftlicher Zeitschriftde_CH
dcterms.typeTextde_CH
zhaw.departementLife Sciences und Facility Managementde_CH
zhaw.organisationalunitInstitut für Chemie und Biotechnologie (ICBT)de_CH
dc.identifier.doi10.1179/1743132814Y.0000000401de_CH
dc.identifier.pmid24923209de_CH
zhaw.funding.euNode_CH
zhaw.issue11de_CH
zhaw.originated.zhawYesde_CH
zhaw.pages.end1010de_CH
zhaw.pages.start1001de_CH
zhaw.publication.statuspublishedVersionde_CH
zhaw.volume36de_CH
zhaw.publication.reviewPeer review (Publikation)de_CH
zhaw.webfeedPharmazeutische Technologiede_CH
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Albert, I., Hefti, M., & Luginbühl, V. (2014). Physiological oxygen concentration alters glioma cell malignancy and responsiveness to photodynamic therapy in vitro. Neurological Research, 36(11), 1001–1010. https://doi.org/10.1179/1743132814Y.0000000401
Albert, I., Hefti, M. and Luginbühl, V. (2014) ‘Physiological oxygen concentration alters glioma cell malignancy and responsiveness to photodynamic therapy in vitro’, Neurological Research, 36(11), pp. 1001–1010. Available at: https://doi.org/10.1179/1743132814Y.0000000401.
I. Albert, M. Hefti, and V. Luginbühl, “Physiological oxygen concentration alters glioma cell malignancy and responsiveness to photodynamic therapy in vitro,” Neurological Research, vol. 36, no. 11, pp. 1001–1010, 2014, doi: 10.1179/1743132814Y.0000000401.
ALBERT, Ina, Martin HEFTI und Vera LUGINBÜHL, 2014. Physiological oxygen concentration alters glioma cell malignancy and responsiveness to photodynamic therapy in vitro. Neurological Research. 2014. Bd. 36, Nr. 11, S. 1001–1010. DOI 10.1179/1743132814Y.0000000401
Albert, Ina, Martin Hefti, and Vera Luginbühl. 2014. “Physiological Oxygen Concentration Alters Glioma Cell Malignancy and Responsiveness to Photodynamic Therapy in Vitro.” Neurological Research 36 (11): 1001–10. https://doi.org/10.1179/1743132814Y.0000000401.
Albert, Ina, et al. “Physiological Oxygen Concentration Alters Glioma Cell Malignancy and Responsiveness to Photodynamic Therapy in Vitro.” Neurological Research, vol. 36, no. 11, 2014, pp. 1001–10, https://doi.org/10.1179/1743132814Y.0000000401.


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