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dc.contributor.authorDoyscher, Dominik-
dc.contributor.authorFieseler, Lars-
dc.contributor.authorDons, Lone-
dc.contributor.authorLoessner, Martin J.-
dc.contributor.authorSchuppler, Markus-
dc.date.accessioned2018-10-29T13:31:55Z-
dc.date.available2018-10-29T13:31:55Z-
dc.date.issued2013-
dc.identifier.issn1462-2912de_CH
dc.identifier.issn1462-2920de_CH
dc.identifier.urihttps://digitalcollection.zhaw.ch/handle/11475/12251-
dc.description.abstractDespite its prominent role as an intracellular human pathogen, Listeria monocytogenes normally features a saprophytic lifestyle, and shares many environmental habitats with predatory protozoa. Earlier studies claimed that Acanthamoeba may act as environmental reservoirs for L. monocytogenes, whereas others failed to confirm this hypothesis. Our findings support the latter and provide clear evidence that L. monocytogenes is unable to persist in Acanthamoeba castellanii and A. polyphaga. Instead, external Listeria cells are rapidly immobilized on the surface of Acanthamoeba trophozoites, forming large aggregates of densely packed bacteria that we termed backpacks. While the assembly of backpacks is dependent on bacterial motility, flagellation alone is not sufficient. Electron micrographs showed that the aggregates are held together by filaments of likely amoebal origin. Time-lapse microscopy revealed that shortly after the bacteria are collected, the amoeba can change direction of movement, phagocytose the backpack and continue to repeat the process. The phenomenon was also observed with avirulent L. monocytogenes mutants, non-pathogenic Listeria, and other motile bacteria, indicating that formation of backpacks is not specific for L. monocytogenes, and independent of bacterial pathogenicity or virulence. Hence, backpacking appears to represent a unique and highly effective strategy of Acanthamoeba to trap and feed on motile bacteria.de_CH
dc.language.isoende_CH
dc.publisherWileyde_CH
dc.relation.ispartofEnvironmental Microbiologyde_CH
dc.rightsLicence according to publishing contractde_CH
dc.subjectAcanthamoebade_CH
dc.subjectBacteriade_CH
dc.subjectListeria monocytogenesde_CH
dc.subjectMicrobial viabilityde_CH
dc.subjectPhagocytosisde_CH
dc.subject.ddc570: Biologiede_CH
dc.titleAcanthamoeba feature a unique backpacking strategy to trap and feed on Listeria monocytogenes and other motile bacteriade_CH
dc.typeBeitrag in wissenschaftlicher Zeitschriftde_CH
dcterms.typeTextde_CH
zhaw.departementLife Sciences und Facility Managementde_CH
zhaw.organisationalunitInstitut für Lebensmittel- und Getränkeinnovation (ILGI)de_CH
dc.identifier.doi10.1111/j.1462-2920.2012.02858.xde_CH
dc.identifier.pmid22925311de_CH
zhaw.funding.euNode_CH
zhaw.issue2de_CH
zhaw.originated.zhawYesde_CH
zhaw.pages.end446de_CH
zhaw.pages.start433de_CH
zhaw.publication.statuspublishedVersionde_CH
zhaw.volume15de_CH
zhaw.publication.reviewPeer review (Publikation)de_CH
zhaw.webfeedMikrobiologiede_CH
Appears in Collections:Publikationen Life Sciences und Facility Management

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