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dc.contributor.authorKumar, Pramod-
dc.contributor.authorSatyam, Abhigyan-
dc.contributor.authorFan, Xingliang-
dc.contributor.authorRochev, Yury-
dc.contributor.authorRodriguez, Brian J.-
dc.contributor.authorGorelov, Alexander-
dc.contributor.authorJoshi, Lokesh-
dc.contributor.authorRaghunath, Michael-
dc.contributor.authorPandit, Abhay-
dc.contributor.authorZeugolis, Dimitrios I.-
dc.date.accessioned2018-10-26T14:41:31Z-
dc.date.available2018-10-26T14:41:31Z-
dc.date.issued2015-
dc.identifier.issn1937-3392de_CH
dc.identifier.issn1937-3384de_CH
dc.identifier.urihttps://digitalcollection.zhaw.ch/handle/11475/12204-
dc.description.abstractTissue engineering by self-assembly uses the cells' secretome as a regeneration template and biological factory of trophic factors. Despite the several advantages that have been witnessed in preclinical and clinical settings, the major obstacle for wide acceptance of this technology remains the tardy extracellular matrix formation. In this study, we assessed the influence of macromolecular crowding (MMC)/excluding volume effect, a biophysical phenomenon that accelerates thermodynamic activities and biological processes by several orders of magnitude, in human corneal fibroblast (HCF) culture. Our data indicate that the addition of negatively charged galactose derivative (carrageenan) in HCF culture, even at 0.5% serum, increases by 12-fold tissue-specific matrix deposition, while maintaining physiological cell morphology and protein/gene expression. Gene analysis indicates that a glucose derivative (dextran sulfate) may drive corneal fibroblasts toward a myofibroblast lineage. Collectively, these results indicate that MMC may be suitable not only for clinical translation and commercialization of tissue engineering by self-assembly therapies, but also for the development of in vitro pathophysiology models.de_CH
dc.language.isoende_CH
dc.publisherMary Ann Liebertde_CH
dc.relation.ispartofTissue Engineering - Part C: Methodsde_CH
dc.rightsLicence according to publishing contractde_CH
dc.subject.ddc571: Physiologie und verwandte Themende_CH
dc.subject.ddc610: Medizin und Gesundheitde_CH
dc.titleAccelerated development of supramolecular corneal stromal-like assemblies from corneal fibroblasts in the presence of macromolecular crowdersde_CH
dc.typeBeitrag in wissenschaftlicher Zeitschriftde_CH
dcterms.typeTextde_CH
zhaw.departementLife Sciences und Facility Managementde_CH
zhaw.organisationalunitInstitut für Chemie und Biotechnologie (ICBT)de_CH
dc.identifier.doi10.1089/ten.TEC.2014.0387de_CH
zhaw.funding.euNode_CH
zhaw.issue7de_CH
zhaw.originated.zhawNode_CH
zhaw.publication.statuspublishedVersionde_CH
zhaw.volume21de_CH
zhaw.publication.reviewPeer review (Publikation)de_CH
zhaw.webfeedMetabolic Tissue Engineeringde_CH
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Kumar, P., Satyam, A., Fan, X., Rochev, Y., Rodriguez, B. J., Gorelov, A., Joshi, L., Raghunath, M., Pandit, A., & Zeugolis, D. I. (2015). Accelerated development of supramolecular corneal stromal-like assemblies from corneal fibroblasts in the presence of macromolecular crowders. Tissue Engineering - Part C: Methods, 21(7). https://doi.org/10.1089/ten.TEC.2014.0387
Kumar, P. et al. (2015) ‘Accelerated development of supramolecular corneal stromal-like assemblies from corneal fibroblasts in the presence of macromolecular crowders’, Tissue Engineering - Part C: Methods, 21(7). Available at: https://doi.org/10.1089/ten.TEC.2014.0387.
P. Kumar et al., “Accelerated development of supramolecular corneal stromal-like assemblies from corneal fibroblasts in the presence of macromolecular crowders,” Tissue Engineering - Part C: Methods, vol. 21, no. 7, 2015, doi: 10.1089/ten.TEC.2014.0387.
KUMAR, Pramod, Abhigyan SATYAM, Xingliang FAN, Yury ROCHEV, Brian J. RODRIGUEZ, Alexander GORELOV, Lokesh JOSHI, Michael RAGHUNATH, Abhay PANDIT und Dimitrios I. ZEUGOLIS, 2015. Accelerated development of supramolecular corneal stromal-like assemblies from corneal fibroblasts in the presence of macromolecular crowders. Tissue Engineering - Part C: Methods. 2015. Bd. 21, Nr. 7. DOI 10.1089/ten.TEC.2014.0387
Kumar, Pramod, Abhigyan Satyam, Xingliang Fan, Yury Rochev, Brian J. Rodriguez, Alexander Gorelov, Lokesh Joshi, Michael Raghunath, Abhay Pandit, and Dimitrios I. Zeugolis. 2015. “Accelerated Development of Supramolecular Corneal Stromal-like Assemblies from Corneal Fibroblasts in the Presence of Macromolecular Crowders.” Tissue Engineering - Part C: Methods 21 (7). https://doi.org/10.1089/ten.TEC.2014.0387.
Kumar, Pramod, et al. “Accelerated Development of Supramolecular Corneal Stromal-like Assemblies from Corneal Fibroblasts in the Presence of Macromolecular Crowders.” Tissue Engineering - Part C: Methods, vol. 21, no. 7, 2015, https://doi.org/10.1089/ten.TEC.2014.0387.


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