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dc.contributor.authorZeugolis, D. I.-
dc.contributor.authorPanengad, P. P.-
dc.contributor.authorYew, E. S. Y.-
dc.contributor.authorSheppard, C.-
dc.contributor.authorPhan, T. T.-
dc.contributor.authorRaghunath, M.-
dc.date.accessioned2018-10-26T14:31:03Z-
dc.date.available2018-10-26T14:31:03Z-
dc.date.issued2009-
dc.identifier.issn1552-4965de_CH
dc.identifier.urihttps://digitalcollection.zhaw.ch/handle/11475/12202-
dc.description.abstractTransglutaminases (TGases) constitute a family of enzymes that stabilize protein assemblies by γ‐glutamyl‐ε‐lysine crosslinks. The role of tissue transglutaminase (TGase 2) in several pathophysiologies, wound healing applications, biomaterials functionalization, and drug delivery systems provides grounds for its use in tissue engineering. Herein, we initially studied the endogenous TGase activity and expression under normal (skin, duodenum, colon, and small bowel) and pathophysiological (keloid scar) conditions on cadaveric human tissues. Successful inhibition was achieved using low concentrations of BOC‐DON‐QIV‐OMe (0.1 mM and 1 mM for normal skin and keloid scar, respectively), iodoacetamide (0.1 mM and 1 mM for normal skin and keloid scar, respectively), and cystamine dihydrochloride (1 mM and 10 mM for normal skin and keloid scar, respectively), whilst di‐BOC‐cystamine was found ineffective even at 100 mM concentration. Secondly, the addition of exogenous guinea pig liver transglutaminase (gpTGase) onto the inhibited tissues and collagen scaffolds was studied, and results presented advocate its use as potential tissue adhesive and drug delivery tool. However, the investigation of its crosslinking extent using second harmonic generation microscopy and differentially scanning calorimetry revealed rather poor stabilization function. Overall, our study indicates that TGase 2 has a role as a biological glue to consolidate various micro‐structural components of tissues and biomaterials.de_CH
dc.language.isoende_CH
dc.publisherWileyde_CH
dc.relation.ispartofJournal of Biomedical Materials Researchde_CH
dc.rightsLicence according to publishing contractde_CH
dc.subject.ddc571: Physiologie und verwandte Themende_CH
dc.subject.ddc610: Medizin und Gesundheitde_CH
dc.titleAn in situ and in vitro investigation for the transglutaminase potential in tissue engineeringde_CH
dc.typeBeitrag in wissenschaftlicher Zeitschriftde_CH
dcterms.typeTextde_CH
zhaw.departementLife Sciences und Facility Managementde_CH
zhaw.organisationalunitInstitut für Chemie und Biotechnologie (ICBT)de_CH
dc.identifier.doi10.1002/jbm.a.32383de_CH
zhaw.funding.euNode_CH
zhaw.issue4de_CH
zhaw.originated.zhawNode_CH
zhaw.pages.end1320de_CH
zhaw.pages.start1310de_CH
zhaw.publication.statuspublishedVersionde_CH
zhaw.volume92Ade_CH
zhaw.publication.reviewPeer review (Publikation)de_CH
zhaw.webfeedMetabolic Tissue Engineeringde_CH
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Zeugolis, D. I., Panengad, P. P., Yew, E. S. Y., Sheppard, C., Phan, T. T., & Raghunath, M. (2009). An in situ and in vitro investigation for the transglutaminase potential in tissue engineering. Journal of Biomedical Materials Research, 92A(4), 1310–1320. https://doi.org/10.1002/jbm.a.32383
Zeugolis, D.I. et al. (2009) ‘An in situ and in vitro investigation for the transglutaminase potential in tissue engineering’, Journal of Biomedical Materials Research, 92A(4), pp. 1310–1320. Available at: https://doi.org/10.1002/jbm.a.32383.
D. I. Zeugolis, P. P. Panengad, E. S. Y. Yew, C. Sheppard, T. T. Phan, and M. Raghunath, “An in situ and in vitro investigation for the transglutaminase potential in tissue engineering,” Journal of Biomedical Materials Research, vol. 92A, no. 4, pp. 1310–1320, 2009, doi: 10.1002/jbm.a.32383.
ZEUGOLIS, D. I., P. P. PANENGAD, E. S. Y. YEW, C. SHEPPARD, T. T. PHAN und M. RAGHUNATH, 2009. An in situ and in vitro investigation for the transglutaminase potential in tissue engineering. Journal of Biomedical Materials Research. 2009. Bd. 92A, Nr. 4, S. 1310–1320. DOI 10.1002/jbm.a.32383
Zeugolis, D. I., P. P. Panengad, E. S. Y. Yew, C. Sheppard, T. T. Phan, and M. Raghunath. 2009. “An in Situ and in Vitro Investigation for the Transglutaminase Potential in Tissue Engineering.” Journal of Biomedical Materials Research 92A (4): 1310–20. https://doi.org/10.1002/jbm.a.32383.
Zeugolis, D. I., et al. “An in Situ and in Vitro Investigation for the Transglutaminase Potential in Tissue Engineering.” Journal of Biomedical Materials Research, vol. 92A, no. 4, 2009, pp. 1310–20, https://doi.org/10.1002/jbm.a.32383.


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