Establishing criteria for human mesenchymal stem cell potency

Samsonraj, Rebekah M.; Rai, Bina; Sathiyanathan, Padmapriya; Puan, Kia Joo; Rötzschke, Olaf; Hui, James H.; Raghunath, Michael; Stanton, Lawrence W.; Nurcombe, Victor; Cool, Simon M.

doi:10.21256/zhaw-4753

Please use this identifier to cite or link to this item: https://doi.org/10.21256/zhaw-4753

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DC Field	Value	Language
dc.contributor.author	Samsonraj, Rebekah M.	-
dc.contributor.author	Rai, Bina	-
dc.contributor.author	Sathiyanathan, Padmapriya	-
dc.contributor.author	Puan, Kia Joo	-
dc.contributor.author	Rötzschke, Olaf	-
dc.contributor.author	Hui, James H.	-
dc.contributor.author	Raghunath, Michael	-
dc.contributor.author	Stanton, Lawrence W.	-
dc.contributor.author	Nurcombe, Victor	-
dc.contributor.author	Cool, Simon M.	-
dc.date.accessioned	2018-10-26T14:16:10Z	-
dc.date.available	2018-10-26T14:16:10Z	-
dc.date.issued	2015	-
dc.identifier.issn	1549-4918	de_CH
dc.identifier.uri	https://digitalcollection.zhaw.ch/handle/11475/12197	-
dc.description.abstract	This study sought to identify critical determinants of mesenchymal stem cell (MSC) potency using in vitro and in vivo attributes of cells isolated from the bone marrow of age‐ and sex‐matched donors. Adherence to plastic was not indicative of potency, yet capacity for long‐term expansion in vitro varied considerably between donors, allowing the grouping of MSCs from the donors into either those with high‐growth capacity or low‐growth capacity. Using this grouping strategy, high‐growth capacity MSCs were smaller in size, had greater colony‐forming efficiency, and had longer telomeres. Cell‐surface biomarker analysis revealed that the International Society for Cellular Therapy (ISCT) criteria did not distinguish between high‐growth capacity and low‐growth capacity MSCs, whereas STRO‐1 and platelet‐derived growth factor receptor alpha were preferentially expressed on high‐growth capacity MSCs. These cells also had the highest mean expression of the mRNA transcripts TWIST‐1 and DERMO‐1. Irrespective of these differences, both groups of donor MSCs produced similar levels of key growth factors and cytokines involved in tissue regeneration and were capable of multilineage differentiation. However, high‐growth capacity MSCs produced approximately double the volume of mineralized tissue compared to low‐growth capacity MSCs when assessed for ectopic bone‐forming ability. The additional phenotypic criteria presented in this study when combined with the existing ISCT minimum criteria and working proposal will permit an improved assessment of MSC potency and provide a basis for establishing the quality of MSCs prior to their therapeutic application.	de_CH
dc.language.iso	en	de_CH
dc.publisher	Wiley	de_CH
dc.relation.ispartof	Stem Cells	de_CH
dc.rights	http://creativecommons.org/licenses/by/4.0/	de_CH
dc.subject.ddc	616: Innere Medizin und Krankheiten	de_CH
dc.title	Establishing criteria for human mesenchymal stem cell potency	de_CH
dc.type	Beitrag in wissenschaftlicher Zeitschrift	de_CH
dcterms.type	Text	de_CH
zhaw.departement	Life Sciences und Facility Management	de_CH
zhaw.organisationalunit	Institut für Chemie und Biotechnologie (ICBT)	de_CH
dc.identifier.doi	10.21256/zhaw-4753	-
dc.identifier.doi	10.1002/stem.1982	de_CH
zhaw.funding.eu	No	de_CH
zhaw.issue	6	de_CH
zhaw.originated.zhaw	No	de_CH
zhaw.pages.end	1891	de_CH
zhaw.pages.start	1878	de_CH
zhaw.publication.status	publishedVersion	de_CH
zhaw.volume	33	de_CH
zhaw.publication.review	Peer review (Publikation)	de_CH
zhaw.webfeed	Metabolic Tissue Engineering	de_CH
Appears in collections:	Publikationen Life Sciences und Facility Management

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Samsonraj, R. M., Rai, B., Sathiyanathan, P., Puan, K. J., Rötzschke, O., Hui, J. H., Raghunath, M., Stanton, L. W., Nurcombe, V., & Cool, S. M. (2015). Establishing criteria for human mesenchymal stem cell potency. Stem Cells, 33(6), 1878–1891. https://doi.org/10.21256/zhaw-4753

Samsonraj, R.M. et al. (2015) ‘Establishing criteria for human mesenchymal stem cell potency’, Stem Cells, 33(6), pp. 1878–1891. Available at: https://doi.org/10.21256/zhaw-4753.

R. M. Samsonraj et al., “Establishing criteria for human mesenchymal stem cell potency,” Stem Cells, vol. 33, no. 6, pp. 1878–1891, 2015, doi: 10.21256/zhaw-4753.

SAMSONRAJ, Rebekah M., Bina RAI, Padmapriya SATHIYANATHAN, Kia Joo PUAN, Olaf RÖTZSCHKE, James H. HUI, Michael RAGHUNATH, Lawrence W. STANTON, Victor NURCOMBE und Simon M. COOL, 2015. Establishing criteria for human mesenchymal stem cell potency. Stem Cells. 2015. Bd. 33, Nr. 6, S. 1878–1891. DOI 10.21256/zhaw-4753

Samsonraj, Rebekah M., Bina Rai, Padmapriya Sathiyanathan, Kia Joo Puan, Olaf Rötzschke, James H. Hui, Michael Raghunath, Lawrence W. Stanton, Victor Nurcombe, and Simon M. Cool. 2015. “Establishing Criteria for Human Mesenchymal Stem Cell Potency.” Stem Cells 33 (6): 1878–91. https://doi.org/10.21256/zhaw-4753.

Samsonraj, Rebekah M., et al. “Establishing Criteria for Human Mesenchymal Stem Cell Potency.” Stem Cells, vol. 33, no. 6, 2015, pp. 1878–91, https://doi.org/10.21256/zhaw-4753.