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  4. Publikationen Life Sciences und Facility Management
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dc.contributor.authorSamsonraj, Rebekah M-
dc.contributor.authorRaghunath, Michael-
dc.contributor.authorHui, James H-
dc.contributor.authorLing, Ling-
dc.contributor.authorNurcombe, Victor-
dc.contributor.authorCool, Simon M-
dc.date.accessioned2018-10-23T14:33:01Z-
dc.date.available2018-10-23T14:33:01Z-
dc.date.issued2013-05-01-
dc.identifier.issn1879-0038de_CH
dc.identifier.issn0378-1119de_CH
dc.identifier.urihttps://digitalcollection.zhaw.ch/handle/11475/12071-
dc.description.abstractHuman mesenchymal stem cells (hMSCs) have attracted much attention for tissue repair and wound healing because of their self-renewal capacity and multipotentiality. In order to mediate an effective therapy, substantial numbers of cells are required, which necessitates extensive sub-culturing and expansion of hMSCs. Throughout ex vivo expansion, the cells undergo telomere shortening, and critically short telomeres can trigger loss of cell viability. Telomeres are nucleoprotein structures that cap the ends of chromosomes, and serve to protect the DNA from the degradation which occurs due to the end-replication problem in all eukaryotes. As hMSCs have only a finite ability for self-renewal like most somatic cells, assaying for telomere length in hMSCs provides critical information on the replicative capacity of the cells, an important criterion in the selection of hMSCs for therapy. Telomere length is generally quantified by Southern blotting and fluorescence in situ hybridization, and more recently by PCR-based methods. Here we describe the quantification of hMSC telomere length by real-time PCR; our results demonstrate the effect of telomere shortening on the proliferation and clonogenicity of hMSCs. Thus, this assay constitutes a useful tool for the determination of relative telomere length in hMSCs.de_CH
dc.language.isoende_CH
dc.publisherElsevierde_CH
dc.relation.ispartofGenede_CH
dc.rightsLicence according to publishing contractde_CH
dc.subjectSouthern blottingde_CH
dc.subjectCell proliferationde_CH
dc.subjectCell survivalde_CH
dc.subjectHuman chromosomesde_CH
dc.subjectDNAde_CH
dc.subjectGene dosagede_CH
dc.subjectGene expressionde_CH
dc.subjectFluorescence in situ hybridizationde_CH
dc.subjectMesenchymal stromal cellsde_CH
dc.subjectReal-time polymerase chain reactionde_CH
dc.subjectTelomerasede_CH
dc.subjectTelomere shorteningde_CH
dc.subject.ddc570: Biologiede_CH
dc.subject.ddc610: Medizin und Gesundheitde_CH
dc.titleTelomere length analysis of human mesenchymal stem cells by quantitative PCRde_CH
dc.typeBeitrag in wissenschaftlicher Zeitschriftde_CH
dcterms.typeTextde_CH
zhaw.departementLife Sciences und Facility Managementde_CH
zhaw.organisationalunitInstitut für Chemie und Biotechnologie (ICBT)de_CH
dc.identifier.doi10.1016/j.gene.2013.01.039de_CH
zhaw.funding.euNode_CH
zhaw.issue2de_CH
zhaw.originated.zhawNode_CH
zhaw.pages.end355de_CH
zhaw.pages.start348de_CH
zhaw.publication.statuspublishedVersionde_CH
zhaw.volume519de_CH
zhaw.publication.reviewPeer review (Publikation)de_CH
zhaw.webfeedMetabolic Tissue Engineeringde_CH
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Samsonraj, R. M., Raghunath, M., Hui, J. H., Ling, L., Nurcombe, V., & Cool, S. M. (2013). Telomere length analysis of human mesenchymal stem cells by quantitative PCR. Gene, 519(2), 348–355. https://doi.org/10.1016/j.gene.2013.01.039
Samsonraj, R.M. et al. (2013) ‘Telomere length analysis of human mesenchymal stem cells by quantitative PCR’, Gene, 519(2), pp. 348–355. Available at: https://doi.org/10.1016/j.gene.2013.01.039.
R. M. Samsonraj, M. Raghunath, J. H. Hui, L. Ling, V. Nurcombe, and S. M. Cool, “Telomere length analysis of human mesenchymal stem cells by quantitative PCR,” Gene, vol. 519, no. 2, pp. 348–355, May 2013, doi: 10.1016/j.gene.2013.01.039.
SAMSONRAJ, Rebekah M, Michael RAGHUNATH, James H HUI, Ling LING, Victor NURCOMBE und Simon M COOL, 2013. Telomere length analysis of human mesenchymal stem cells by quantitative PCR. Gene. 1 Mai 2013. Bd. 519, Nr. 2, S. 348–355. DOI 10.1016/j.gene.2013.01.039
Samsonraj, Rebekah M, Michael Raghunath, James H Hui, Ling Ling, Victor Nurcombe, and Simon M Cool. 2013. “Telomere Length Analysis of Human Mesenchymal Stem Cells by Quantitative PCR.” Gene 519 (2): 348–55. https://doi.org/10.1016/j.gene.2013.01.039.
Samsonraj, Rebekah M., et al. “Telomere Length Analysis of Human Mesenchymal Stem Cells by Quantitative PCR.” Gene, vol. 519, no. 2, May 2013, pp. 348–55, https://doi.org/10.1016/j.gene.2013.01.039.


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