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  4. Publikationen Life Sciences und Facility Management
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dc.contributor.authorRuth, Claudia-
dc.contributor.authorZuellig, Thomas-
dc.contributor.authorMellitzer, Andrea-
dc.contributor.authorWeiss, Roland-
dc.contributor.authorLooser, Verena-
dc.contributor.authorKovar, Karin-
dc.contributor.authorGlieder, Anton-
dc.date.accessioned2018-10-08T11:42:29Z-
dc.date.available2018-10-08T11:42:29Z-
dc.date.issued2010-05-29-
dc.identifier.issn1872-5325de_CH
dc.identifier.urihttps://digitalcollection.zhaw.ch/handle/11475/11466-
dc.identifier.urihttp://europepmc.org/articles/PMC2955199?pdf=renderde_CH
dc.description.abstractNatural tools for recombinant protein production show technological limitations. Available natural promoters for gene expression in Pichia pastoris are either constitutive, weak or require the use of undesirable substances or procedures for induction. Here we show the application of deletion variants based on the well known methanol inducible AOX1 promoter and small synthetic promoters, where cis-acting elements were fused to core promoter fragments. They enable differently regulated target protein expression and at the same time to replace methanol induction by a glucose or glycerol feeding strategy. Trypsinogen, the precursor of the serine protease trypsin, was expressed using these different promoters. Depending on the applied promoter the production window (i.e. the time of increasing product concentration) changed significantly. In fedbatch processes trypsinogen yields before induction with methanol were up to 10 times higher if variants of the AOX1 promoter were applied. In addition, the starting point of autoproteolytic product degradation can be predetermined by the promoter choice.  de_CH
dc.language.isoende_CH
dc.publisherSpringerde_CH
dc.relation.ispartofSystems and Synthetic Biologyde_CH
dc.rightsLicence according to publishing contractde_CH
dc.subjectPorcine trypsinogende_CH
dc.subjectProduction windowde_CH
dc.subjectPichia pastorisde_CH
dc.subjectAOX1 promoter variantsde_CH
dc.subject.ddc660.6: Biotechnologiede_CH
dc.titleVariable production windows for porcine trypsinogen employing synthetic inducible promoter variants in Pichia pastorisde_CH
dc.typeBeitrag in wissenschaftlicher Zeitschriftde_CH
dcterms.typeTextde_CH
zhaw.departementLife Sciences und Facility Managementde_CH
zhaw.organisationalunitInstitut für Chemie und Biotechnologie (ICBT)de_CH
dc.identifier.doi10.1007/s11693-010-9057-0de_CH
zhaw.funding.euNode_CH
zhaw.issue3de_CH
zhaw.originated.zhawYesde_CH
zhaw.pages.end191de_CH
zhaw.pages.start181de_CH
zhaw.publication.statuspublishedVersionde_CH
zhaw.volume4de_CH
zhaw.publication.reviewPeer review (Publikation)de_CH
zhaw.webfeedBiokatalysede_CH
Appears in collections:Publikationen Life Sciences und Facility Management

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Ruth, C., Zuellig, T., Mellitzer, A., Weiss, R., Looser, V., Kovar, K., & Glieder, A. (2010). Variable production windows for porcine trypsinogen employing synthetic inducible promoter variants in Pichia pastoris. Systems and Synthetic Biology, 4(3), 181–191. https://doi.org/10.1007/s11693-010-9057-0
Ruth, C. et al. (2010) ‘Variable production windows for porcine trypsinogen employing synthetic inducible promoter variants in Pichia pastoris’, Systems and Synthetic Biology, 4(3), pp. 181–191. Available at: https://doi.org/10.1007/s11693-010-9057-0.
C. Ruth et al., “Variable production windows for porcine trypsinogen employing synthetic inducible promoter variants in Pichia pastoris,” Systems and Synthetic Biology, vol. 4, no. 3, pp. 181–191, May 2010, doi: 10.1007/s11693-010-9057-0.
RUTH, Claudia, Thomas ZUELLIG, Andrea MELLITZER, Roland WEISS, Verena LOOSER, Karin KOVAR und Anton GLIEDER, 2010. Variable production windows for porcine trypsinogen employing synthetic inducible promoter variants in Pichia pastoris. Systems and Synthetic Biology [online]. 29 Mai 2010. Bd. 4, Nr. 3, S. 181–191. DOI 10.1007/s11693-010-9057-0. Verfügbar unter: http://europepmc.org/articles/PMC2955199?pdf=render
Ruth, Claudia, Thomas Zuellig, Andrea Mellitzer, Roland Weiss, Verena Looser, Karin Kovar, and Anton Glieder. 2010. “Variable Production Windows for Porcine Trypsinogen Employing Synthetic Inducible Promoter Variants in Pichia Pastoris.” Systems and Synthetic Biology 4 (3): 181–91. https://doi.org/10.1007/s11693-010-9057-0.
Ruth, Claudia, et al. “Variable Production Windows for Porcine Trypsinogen Employing Synthetic Inducible Promoter Variants in Pichia Pastoris.” Systems and Synthetic Biology, vol. 4, no. 3, May 2010, pp. 181–91, https://doi.org/10.1007/s11693-010-9057-0.


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