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Please use this identifier to cite or link to this item: https://doi.org/10.21256/zhaw-25803
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dc.contributor.authorJoshi, Akshay-
dc.contributor.authorYoung, Diana-
dc.contributor.authorHuang, Liren-
dc.contributor.authorMosberger, Lona-
dc.contributor.authorMunk, Bernhard-
dc.contributor.authorVinzelj, Julia-
dc.contributor.authorFlad, Veronika-
dc.contributor.authorSczyrba, Alexander-
dc.contributor.authorGriffith, Gareth W.-
dc.contributor.authorPodmirseg, Sabine Marie-
dc.contributor.authorWarthmann, Rolf-
dc.contributor.authorLebuhn, Michael-
dc.contributor.authorInsam, Heribert-
dc.date.accessioned2022-10-14T12:25:41Z-
dc.date.available2022-10-14T12:25:41Z-
dc.date.issued2022-10-
dc.identifier.issn2076-2607de_CH
dc.identifier.urihttps://digitalcollection.zhaw.ch/handle/11475/25803-
dc.description.abstractAnaerobic fungi (AF), belonging to the phylum Neocallimastigomycota, are a pivotal component of the digestive tract microbiome of various herbivorous animals. In the last decade, the diversity of AF has rapidly expanded due to the exploration of numerous (novel) habitats. Studies aiming at understanding the role of AF require robust and reliable isolation and cultivation techniques, many of which remained unchanged for decades. Using amplicon sequencing, we compared three different media: medium with rumen fluid (RF), depleted rumen fluid (DRF), and no rumen fluid (NRF) to enrich the AF from the feces of yak, as a rumen control; and Przewalski’s horse, llama, guanaco, and elephant, as a non-rumen habitats. The results revealed the selective enrichment of Piromyces and Neocallimastix from the feces of elephant and llama, respectively, in the RF medium. Similarly, the enrichment culture in DRF medium explicitly manifested Piromyces-related sequences from elephant feces. Five new clades (MM1-5) were defined from llama, guanaco, yak, and elephant feces that could as well be enriched from llama and elephant samples using non-conventional DRF and NRF media. This study presents evidence for the selective enrichment of certain genera in medium with RF and DRF from rumen as well as from non-rumen samples. NRF medium is suggested for the isolation of AF from non-rumen environments.de_CH
dc.language.isoende_CH
dc.publisherMDPIde_CH
dc.relation.ispartofMicroorganismsde_CH
dc.rightshttp://creativecommons.org/licenses/by/4.0/de_CH
dc.subjectMicrobiologyde_CH
dc.subjectAnaerobic fungusde_CH
dc.subjectNeocallimastigomycotade_CH
dc.subjectRuminantde_CH
dc.subjectHindgut animalde_CH
dc.subjectMinimal mediumde_CH
dc.subjectNovel genusde_CH
dc.subjectSelective isolationde_CH
dc.subjectEnrichmentde_CH
dc.subject.ddc579: Mikrobiologiede_CH
dc.titleEffect of growth media on the diversity of Neocallimastigomycetes from non-rumen habitatsde_CH
dc.typeBeitrag in wissenschaftlicher Zeitschriftde_CH
dcterms.typeTextde_CH
zhaw.departementLife Sciences und Facility Managementde_CH
zhaw.organisationalunitInstitut für Chemie und Biotechnologie (ICBT)de_CH
dc.identifier.doi10.3390/microorganisms10101972de_CH
dc.identifier.doi10.21256/zhaw-25803-
zhaw.funding.euNot specifiedde_CH
zhaw.issue10de_CH
zhaw.originated.zhawYesde_CH
zhaw.pages.start1972de_CH
zhaw.publication.statuspublishedVersionde_CH
zhaw.volume10de_CH
zhaw.publication.reviewPeer review (Publikation)de_CH
zhaw.funding.snf179552de_CH
zhaw.webfeedUmweltbiotechnologie und Bioenergiede_CH
zhaw.funding.zhawEntfesselung des versteckten Potentials anaerober Pilze (Neocallimastigomycota)de_CH
zhaw.author.additionalNode_CH
zhaw.display.portraitYesde_CH
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Joshi, A., Young, D., Huang, L., Mosberger, L., Munk, B., Vinzelj, J., Flad, V., Sczyrba, A., Griffith, G. W., Podmirseg, S. M., Warthmann, R., Lebuhn, M., & Insam, H. (2022). Effect of growth media on the diversity of Neocallimastigomycetes from non-rumen habitats. Microorganisms, 10(10), 1972. https://doi.org/10.3390/microorganisms10101972
Joshi, A. et al. (2022) ‘Effect of growth media on the diversity of Neocallimastigomycetes from non-rumen habitats’, Microorganisms, 10(10), p. 1972. Available at: https://doi.org/10.3390/microorganisms10101972.
A. Joshi et al., “Effect of growth media on the diversity of Neocallimastigomycetes from non-rumen habitats,” Microorganisms, vol. 10, no. 10, p. 1972, Oct. 2022, doi: 10.3390/microorganisms10101972.
JOSHI, Akshay, Diana YOUNG, Liren HUANG, Lona MOSBERGER, Bernhard MUNK, Julia VINZELJ, Veronika FLAD, Alexander SCZYRBA, Gareth W. GRIFFITH, Sabine Marie PODMIRSEG, Rolf WARTHMANN, Michael LEBUHN und Heribert INSAM, 2022. Effect of growth media on the diversity of Neocallimastigomycetes from non-rumen habitats. Microorganisms. Oktober 2022. Bd. 10, Nr. 10, S. 1972. DOI 10.3390/microorganisms10101972
Joshi, Akshay, Diana Young, Liren Huang, Lona Mosberger, Bernhard Munk, Julia Vinzelj, Veronika Flad, et al. 2022. “Effect of Growth Media on the Diversity of Neocallimastigomycetes from Non-Rumen Habitats.” Microorganisms 10 (10): 1972. https://doi.org/10.3390/microorganisms10101972.
Joshi, Akshay, et al. “Effect of Growth Media on the Diversity of Neocallimastigomycetes from Non-Rumen Habitats.” Microorganisms, vol. 10, no. 10, Oct. 2022, p. 1972, https://doi.org/10.3390/microorganisms10101972.


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