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https://doi.org/10.21256/zhaw-4754| Publikationstyp: | Beitrag in wissenschaftlicher Zeitschrift |
| Art der Begutachtung: | Peer review (Publikation) |
| Titel: | Macromolecularly crowded in vitro microenvironments accelerate the production of extracellular matrix-rich supramolecular assemblies |
| Autor/-in: | Kumar, Pramod Satyam, Abhigyan Fan, Xingliang Collin, Estelle Rochev, Yury Rodriguez, Brian J. Gorelov, Alexander Dillon, Simon Joshi, Lokesh Raghunath, Michael Pandit, Abhay Zeugolis, Dimitrios I. |
| DOI: | 10.21256/zhaw-4754 10.1038/srep08729 |
| Erschienen in: | Scientific Reports |
| Band(Heft): | 5 |
| Heft: | 8729 |
| Erscheinungsdatum: | 2015 |
| Verlag / Hrsg. Institution: | Nature Publishing Group |
| ISSN: | 2045-2322 |
| Sprache: | Englisch |
| Fachgebiet (DDC): | 571: Physiologie und verwandte Themen 572: Biochemie |
| Zusammenfassung: | Therapeutic strategies based on the principles of tissue engineering by self-assembly put forward the notion that functional regeneration can be achieved by utilising the inherent capacity of cells to create highly sophisticated supramolecular assemblies. However, in dilute ex-vivo microenvironments, prolonged culture time is required to develop an extracellular matrix-rich implantable device. Herein, we assessed the influence of macromolecular crowding, a biophysical phenomenon that regulates intra- and extra-cellular activities in multicellular organisms, in human corneal fibroblast culture. In the presence of macromolecules, abundant extracellular matrix deposition was evidenced as fast as 48 h in culture, even at low serum concentration. Temperature responsive copolymers allowed the detachment of dense and cohesive supramolecularly assembled living substitutes within 6 days in culture. Morphological, histological, gene and protein analysis assays demonstrated maintenance of tissue-specific function. Macromolecular crowding opens new avenues for a more rational design in engineering of clinically relevant tissue modules in vitro. |
| URI: | https://digitalcollection.zhaw.ch/handle/11475/12199 |
| Volltext Version: | Publizierte Version |
| Lizenz (gemäss Verlagsvertrag): | CC BY 4.0: Namensnennung 4.0 International |
| Departement: | Life Sciences und Facility Management |
| Organisationseinheit: | Institut für Chemie und Biotechnologie (ICBT) |
| Enthalten in den Sammlungen: | Publikationen Life Sciences und Facility Management |
Dateien zu dieser Ressource:
| Datei | Beschreibung | Größe | Format | |
|---|---|---|---|---|
| 2015_Kumar_et_al_Macromolecularity_crowded_in_vitro_microenvironments_srep08729.pdf | 4.53 MB | Adobe PDF |  Öffnen/Anzeigen |
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Kumar, P., Satyam, A., Fan, X., Collin, E., Rochev, Y., Rodriguez, B. J., Gorelov, A., Dillon, S., Joshi, L., Raghunath, M., Pandit, A., & Zeugolis, D. I. (2015). Macromolecularly crowded in vitro microenvironments accelerate the production of extracellular matrix-rich supramolecular assemblies. Scientific Reports, 5(8729). https://doi.org/10.21256/zhaw-4754
Kumar, P. et al. (2015) ‘Macromolecularly crowded in vitro microenvironments accelerate the production of extracellular matrix-rich supramolecular assemblies’, Scientific Reports, 5(8729). Available at: https://doi.org/10.21256/zhaw-4754.
P. Kumar et al., “Macromolecularly crowded in vitro microenvironments accelerate the production of extracellular matrix-rich supramolecular assemblies,” Scientific Reports, vol. 5, no. 8729, 2015, doi: 10.21256/zhaw-4754.
KUMAR, Pramod, Abhigyan SATYAM, Xingliang FAN, Estelle COLLIN, Yury ROCHEV, Brian J. RODRIGUEZ, Alexander GORELOV, Simon DILLON, Lokesh JOSHI, Michael RAGHUNATH, Abhay PANDIT und Dimitrios I. ZEUGOLIS, 2015. Macromolecularly crowded in vitro microenvironments accelerate the production of extracellular matrix-rich supramolecular assemblies. Scientific Reports. 2015. Bd. 5, Nr. 8729. DOI 10.21256/zhaw-4754
Kumar, Pramod, Abhigyan Satyam, Xingliang Fan, Estelle Collin, Yury Rochev, Brian J. Rodriguez, Alexander Gorelov, et al. 2015. “Macromolecularly Crowded in Vitro Microenvironments Accelerate the Production of Extracellular Matrix-Rich Supramolecular Assemblies.” Scientific Reports 5 (8729). https://doi.org/10.21256/zhaw-4754.
Kumar, Pramod, et al. “Macromolecularly Crowded in Vitro Microenvironments Accelerate the Production of Extracellular Matrix-Rich Supramolecular Assemblies.” Scientific Reports, vol. 5, no. 8729, 2015, https://doi.org/10.21256/zhaw-4754.
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